FastQCFastQC Report
Thu 26 May 2016
SRR1546196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546196_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1726938
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289551.6766670256836087No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53610.3104338430215792No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGC26250.152003140819184No Hit
GAGAGAGGACGAGAGAAAGAGAGAAAGAGAAAGAGAGAAAGAGAGAAAGA20080.11627516448187486No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17770.10289888808978666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCC302.5284571E-644.00000432
GCCGTAT302.5284571E-644.00000436
CGGAATA551.8189894E-1244.034
AATCCGG800.044.02
CGAACGC207.857583E-444.042
CGAACAC207.857583E-444.020
CGAGTGT254.443773E-544.021
CATGCGG551.8189894E-1244.02
GCGCGTA502.7284841E-1144.07
CGTGACA207.857583E-444.022
ACCGAGA207.857583E-444.018
CAATTCG207.857583E-444.021
CACGACC207.857583E-444.027
TAACGAA408.3164196E-944.011
CGTTCAC254.443773E-544.021
TAGTTCG207.857583E-444.01
CTAACCG502.7284841E-1144.040
TCTACGA207.857583E-444.032
GTATGCG408.3164196E-944.01
CTACGCG408.3164196E-944.01
Produced by FastQC (version 0.11.5)