##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546196_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1726938
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.869534980410414	31.0	31.0	34.0	30.0	34.0
2	32.26657355388555	33.0	31.0	34.0	30.0	34.0
3	32.274622482104164	34.0	31.0	34.0	30.0	34.0
4	35.9664857684526	37.0	35.0	37.0	35.0	37.0
5	35.90171795397403	37.0	35.0	37.0	35.0	37.0
6	35.99024632036587	37.0	35.0	37.0	35.0	37.0
7	36.18922219558548	37.0	35.0	37.0	35.0	37.0
8	36.08710156357669	37.0	35.0	37.0	35.0	37.0
9	37.85184586823615	39.0	38.0	39.0	35.0	39.0
10	37.6088828898316	39.0	37.0	39.0	35.0	39.0
11	37.401245441353424	39.0	37.0	39.0	35.0	39.0
12	36.863278820664085	39.0	35.0	39.0	33.0	39.0
13	36.672225638673766	39.0	35.0	39.0	33.0	39.0
14	37.83973425797568	40.0	35.0	41.0	33.0	41.0
15	38.0366046725476	40.0	35.0	41.0	33.0	41.0
16	38.12033205592789	40.0	35.0	41.0	34.0	41.0
17	38.04142650170417	40.0	35.0	41.0	34.0	41.0
18	37.836072864225585	39.0	36.0	41.0	34.0	41.0
19	37.56428835314296	38.0	36.0	41.0	34.0	41.0
20	37.22399298643032	38.0	35.0	41.0	34.0	41.0
21	37.145579632853064	38.0	35.0	41.0	33.0	41.0
22	37.08257621292716	38.0	35.0	41.0	33.0	41.0
23	37.01037211526992	38.0	35.0	41.0	33.0	41.0
24	36.94988470923681	38.0	35.0	41.0	33.0	41.0
25	36.914754322390266	38.0	35.0	41.0	33.0	41.0
26	36.862864793061476	38.0	35.0	41.0	33.0	41.0
27	36.78012875968911	38.0	35.0	41.0	33.0	41.0
28	36.71221086107318	38.0	35.0	41.0	33.0	41.0
29	36.63227400173023	38.0	35.0	41.0	33.0	41.0
30	36.404551871578484	38.0	35.0	41.0	32.0	41.0
31	36.059568438473185	38.0	35.0	41.0	31.0	41.0
32	35.63957536402581	38.0	35.0	41.0	29.0	41.0
33	35.136063946707985	38.0	35.0	41.0	24.0	41.0
34	34.684049456320956	38.0	35.0	41.0	21.0	41.0
35	34.35528664028471	38.0	34.0	41.0	18.0	41.0
36	34.14642737608414	38.0	34.0	41.0	18.0	41.0
37	34.02817877653975	38.0	34.0	40.0	17.0	41.0
38	33.966946699881525	38.0	34.0	40.0	17.0	41.0
39	33.91040963833097	37.0	34.0	40.0	16.0	41.0
40	33.796421759206176	37.0	34.0	40.0	15.0	41.0
41	33.67830518524695	37.0	34.0	40.0	15.0	41.0
42	33.5732018173206	37.0	34.0	40.0	14.0	41.0
43	33.449185784318836	37.0	33.0	40.0	12.0	41.0
44	33.29025766993372	37.0	33.0	40.0	10.0	41.0
45	33.24898635619808	37.0	33.0	40.0	10.0	41.0
46	33.17200154261473	36.0	33.0	40.0	10.0	41.0
47	33.14020422273411	36.0	33.0	40.0	10.0	41.0
48	33.09213532854103	36.0	33.0	40.0	10.0	41.0
49	33.00702572993356	36.0	33.0	40.0	10.0	41.0
50	32.855947926329726	36.0	33.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	1.0
12	6.0
13	6.0
14	18.0
15	18.0
16	81.0
17	233.0
18	625.0
19	1247.0
20	2294.0
21	3982.0
22	6629.0
23	10636.0
24	17009.0
25	28367.0
26	45290.0
27	54085.0
28	49561.0
29	41764.0
30	39184.0
31	40594.0
32	48176.0
33	62104.0
34	112801.0
35	232445.0
36	100803.0
37	118939.0
38	203696.0
39	506339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.192023106793645	23.939191794957317	28.469927698620335	21.398857399628707
2	34.82487500998877	25.587079559312492	29.423233491879845	10.16481193881888
3	23.51132466828572	23.455040076713814	42.51808692610852	10.515548328891947
4	21.7878117222506	26.400831992810396	41.28156309027886	10.529793194660146
5	19.463871893490094	28.66402847120163	41.064763182001904	10.807336453306373
6	17.546431892748902	37.58646807239172	35.77638571853767	9.090714316321721
7	75.97418089126535	4.533747013500196	18.013732977095877	1.4783391181385783
8	76.51444348320553	4.218217446138773	17.186488455289073	2.0808506153666197
9	72.35662195168558	6.139073898426	18.816425372537985	2.6878787773504316
10	38.51545336312016	29.989495859144917	23.401824500937497	8.09322627679743
11	33.28579254148094	20.680418173669235	35.35025577061828	10.683533514231547
12	31.7008485539145	20.87052343512043	35.78431883483947	11.644309176125605
13	16.0552376518439	36.455159362988134	34.784514556978884	12.705088428189084
14	11.705399962245314	35.1190372786979	38.117928958653984	15.057633800402796
15	9.837990709568032	24.634873979262718	52.66876981107602	12.858365500093228
16	12.72112837866791	22.70069915654181	47.41038763406677	17.167784830723512
17	12.578563909069116	23.353415119708988	38.810831656955834	25.25718931426606
18	15.723783945920466	25.823683305364753	42.782369720279476	15.670163028435299
19	20.209005766275336	26.706285923408945	35.8542692325955	17.23043907772022
20	21.02849088965556	24.17017866304407	39.88533462116185	14.915995826138516
21	16.682590805228674	28.82998694799698	39.079978551633005	15.407443695141342
22	18.154444456025637	25.204552798073816	35.114462707983726	21.52654003791682
23	13.843114228767911	29.34795574595035	36.406228828133955	20.402701197147785
24	13.586011773439463	25.21045920583136	48.28135115447109	12.922177866258084
25	12.323256538451293	29.073481503099707	42.61838004606998	15.98488191237902
26	12.533049825760973	32.693704116766206	37.983818758982665	16.78942729849016
27	13.033299400441706	34.36747584452945	36.866060043846396	15.733164711182452
28	11.134563024266072	30.656514594038697	43.21249517932896	14.99642720236627
29	12.099739538999083	24.971133879733955	41.70439239856903	21.224734182697933
30	14.863417215904681	32.42751042596781	37.260689150392196	15.448383207735308
31	18.764367915929814	31.009335598614424	35.1673308480096	15.058965637446162
32	19.59057013048529	28.46309479552827	35.03953239780467	16.906802676181773
33	20.16169659825657	31.22497738772324	31.467950789200305	17.145375224819883
34	14.91402702355267	28.31033887724979	35.04636530089673	21.729268798300808
35	17.421702458339556	28.198001317939614	34.87450041634384	19.505795807376987
36	22.234440379446163	30.009241790961806	30.85715873992002	16.899159089672008
37	15.957666111927585	32.50852086177963	36.00494053637131	15.528872489921469
38	15.780415973242814	31.50228902253584	34.973114263511484	17.744180740709858
39	16.15912094122661	33.32238910719435	32.74784618787704	17.770643763701997
40	19.03160391397954	28.856623688864335	32.189053689246514	19.922718707909606
41	14.818366380263798	29.606563756197385	33.952869182333124	21.622200681205694
42	17.03315347742652	28.919741183528302	31.537669563122705	22.50943577592247
43	17.165584404304035	29.66632270527373	31.884584159940886	21.283508730481348
44	14.317306122165357	31.327297216228956	33.226091498363	21.129305163242687
45	15.532173129550683	36.3273609127832	29.174701118395678	18.96576483927043
46	18.0179022061012	33.44051726234526	31.455095666433884	17.08648486511965
47	16.983354353196233	31.221039782551546	32.4178980368722	19.37770782738002
48	17.282786064120426	27.521949253534288	35.50469096169058	19.690573720654708
49	18.39278538083012	28.840525832427105	33.422045261613334	19.34464352512945
50	16.670256836087923	32.155352421453465	32.08493877603018	19.08945196642844
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6871.0
1	8171.0
2	9471.0
3	27849.0
4	46227.0
5	34009.5
6	21792.0
7	23997.5
8	26203.0
9	28430.5
10	30658.0
11	31295.5
12	31933.0
13	31599.5
14	31266.0
15	27581.0
16	23896.0
17	22224.5
18	20553.0
19	19417.0
20	18281.0
21	19944.0
22	21607.0
23	21912.5
24	22218.0
25	24770.5
26	27323.0
27	34778.0
28	42233.0
29	43745.0
30	45257.0
31	60004.0
32	74751.0
33	82560.0
34	90369.0
35	95257.5
36	100146.0
37	103802.0
38	107458.0
39	109202.0
40	110946.0
41	127972.5
42	144999.0
43	142100.5
44	139202.0
45	127156.5
46	115111.0
47	107596.0
48	100081.0
49	93014.5
50	85948.0
51	79803.0
52	73658.0
53	59905.0
54	46152.0
55	37984.5
56	29817.0
57	25006.0
58	20195.0
59	18987.0
60	17779.0
61	16058.0
62	14337.0
63	12029.0
64	9721.0
65	8546.0
66	7371.0
67	6244.0
68	5117.0
69	4071.5
70	3026.0
71	2594.5
72	2163.0
73	1740.5
74	1318.0
75	1018.5
76	719.0
77	546.5
78	374.0
79	278.0
80	182.0
81	144.5
82	107.0
83	80.5
84	54.0
85	36.5
86	19.0
87	18.5
88	18.0
89	11.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	2.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1726938.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.49023477771
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.44502119005237	17.867371784844238
2	8.462261737873881	3.8063650647043166
3	3.095399464294185	2.0884878204832193
4	1.59618720115665	1.435944996127555
5	0.977177744291225	1.0988478444331355
6	0.6621132059295867	0.8934648870467191
7	0.503348153148942	0.7924292697472503
8	0.386491713963224	0.6953831509337957
9	0.30992229026087903	0.6273202563731479
>10	3.1957511094100512	16.05584551020605
>50	0.7579490198073096	11.95226251363427
>100	0.5168743402747682	23.829199232131867
>500	0.07180250363097043	11.327946636387946
>1k	0.01918189627686575	5.528576165881386
>5k	2.5921481455223986E-4	0.31253568721109853
>10k+	2.5921481455223986E-4	1.6880191798540123
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28955	1.6766670256836087	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5361	0.3104338430215792	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGC	2625	0.152003140819184	No Hit
GAGAGAGGACGAGAGAAAGAGAGAAAGAGAAAGAGAGAAAGAGAGAAAGA	2008	0.11627516448187486	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1777	0.10289888808978666	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06948715008876984	0.0
2	0.0	0.0	0.0	0.2945097044595695	0.0
3	0.0	0.0	0.0	0.4367267383079184	0.0
4	0.0	0.0	0.0	0.666613393184932	0.0
5	0.0	0.0	0.0	1.1619409614010463	0.0
6	0.0	0.0	0.0	1.590445053615127	0.0
7	0.0	0.0	0.0	1.855943872912635	0.0
8	0.0	0.0	0.0	2.501132061486863	0.0
9	0.0	0.0	0.0	2.742947343795782	0.0
10	0.0	0.0	0.0	3.2203819708640378	0.0
11	0.0	0.0	0.0	3.83945457219657	0.0
12	0.0	0.0	0.0	4.288457373686837	0.0
13	0.0	0.0	0.0	4.450014997643227	0.0
14	0.0	0.0	0.0	4.508268391800979	0.0
15	0.0	0.0	0.0	4.604392282757111	0.0
16	0.0	0.0	0.0	4.8209026612420365	0.0
17	0.0	0.0	0.0	5.079510671489075	0.0
18	0.0	0.0	0.0	5.420750484383342	0.0
19	0.0	0.0	0.0	5.609176473040723	0.0
20	0.0	0.0	0.0	5.848791328930164	0.0
21	0.0	0.0	0.0	6.116201044855114	0.0
22	0.0	0.0	0.0	6.40474643559873	0.0
23	0.0	0.0	0.0	6.7339997151026845	0.0
24	0.0	0.0	0.0	6.966550044066435	0.0
25	0.0	0.0	0.0	7.179122817379663	0.0
26	0.0	0.0	0.0	7.394417170738034	0.0
27	0.0	0.0	0.0	7.611332890931811	0.0
28	0.0	0.0	0.0	7.87295201101603	0.0
29	0.0	0.0	0.0	8.107818578316072	0.0
30	0.0	0.0	0.0	8.401633411274753	0.0
31	5.7905958407308196E-5	0.0	0.0	8.690468331810406	0.0
32	5.7905958407308196E-5	0.0	0.0	8.941722285339717	0.0
33	5.7905958407308196E-5	0.0	0.0	9.19784033937524	0.0
34	5.7905958407308196E-5	0.0	0.0	9.466234456593115	0.0
35	5.7905958407308196E-5	0.0	0.0	9.812801617660854	0.0
36	5.7905958407308196E-5	0.0	0.0	10.082180136171651	0.0
37	5.7905958407308196E-5	0.0	0.0	10.356017413479812	0.0
38	5.7905958407308196E-5	0.0	0.0	10.614972859477295	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCGCC	30	2.5284571E-6	44.000004	32
GCCGTAT	30	2.5284571E-6	44.000004	36
CGGAATA	55	1.8189894E-12	44.0	34
AATCCGG	80	0.0	44.0	2
CGAACGC	20	7.857583E-4	44.0	42
CGAACAC	20	7.857583E-4	44.0	20
CGAGTGT	25	4.443773E-5	44.0	21
CATGCGG	55	1.8189894E-12	44.0	2
GCGCGTA	50	2.7284841E-11	44.0	7
CGTGACA	20	7.857583E-4	44.0	22
ACCGAGA	20	7.857583E-4	44.0	18
CAATTCG	20	7.857583E-4	44.0	21
CACGACC	20	7.857583E-4	44.0	27
TAACGAA	40	8.3164196E-9	44.0	11
CGTTCAC	25	4.443773E-5	44.0	21
TAGTTCG	20	7.857583E-4	44.0	1
CTAACCG	50	2.7284841E-11	44.0	40
TCTACGA	20	7.857583E-4	44.0	32
GTATGCG	40	8.3164196E-9	44.0	1
CTACGCG	40	8.3164196E-9	44.0	1
>>END_MODULE