FastQCFastQC Report
Thu 26 May 2016
SRR1546195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546195_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3950070
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT579051.4659233886994407No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA414441.049196596516011No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA282730.7157594675537395No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA158980.40247388020971775No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG125040.31655135225451697No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA97740.24743865298589648No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA94530.23931221472024547No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA93980.23791983433204983No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA90730.22969213203816644No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA89040.22541372684534705No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA88600.22429982253479053No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC68310.1729336442139No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG54580.1381747665231249No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT52290.13237740090681938No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA52090.13187108076565732No Hit
TATGCAGTTTTCACCATTCTATATTGTAAAGAATATATATCCAGTATGTA50720.12840278779869724No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA48840.12364337847177392No Hit
AGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG43080.10906135840630672No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGC42600.10784619006751778No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA41510.10508674529818458No Hit
AGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG41490.10503611328406838No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC41090.10402347300174426TruSeq Adapter, Index 13 (95% over 21bp)
AAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA40340.10212477247238656No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAC207.8588136E-444.00000429
CAACCGT408.3218765E-944.00000431
CTACGCG408.3218765E-944.0000041
TACGAAC207.8588136E-444.00000412
CCGAGTA207.8588136E-444.00000419
ATACGCG650.044.0000041
TGTACGA207.8588136E-444.00000410
CAATACG207.8588136E-444.0000041
TAATGCG1300.044.0000041
GCCGTAA207.8588136E-444.00000418
TACGTTA207.8588136E-444.00000443
TCGTTAG2900.044.01
GTCGATA1350.044.09
CGTGCAT254.4448178E-544.026
ATTACGC351.4472789E-744.042
TGCGAAC254.4448178E-544.031
GTGTCGA254.4448178E-544.038
CGTACTC302.5292902E-644.07
TTAACGA551.8189894E-1244.038
CGGTATG254.4448178E-544.01
Produced by FastQC (version 0.11.5)