##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546195_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3950070
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93719199912913	31.0	31.0	34.0	30.0	34.0
2	32.28815970349893	33.0	31.0	34.0	30.0	34.0
3	32.21942699749625	34.0	31.0	34.0	30.0	34.0
4	35.95108668960297	37.0	35.0	37.0	35.0	37.0
5	35.91280407688978	37.0	35.0	37.0	35.0	37.0
6	35.95071505061936	37.0	35.0	37.0	35.0	37.0
7	36.16242699496465	37.0	35.0	37.0	35.0	37.0
8	36.026162321174056	37.0	35.0	37.0	35.0	37.0
9	37.69506008754275	39.0	38.0	39.0	35.0	39.0
10	37.52487348325473	39.0	37.0	39.0	35.0	39.0
11	37.42348819134851	39.0	37.0	39.0	35.0	39.0
12	36.93222373274398	39.0	35.0	39.0	33.0	39.0
13	36.74246228547849	39.0	35.0	39.0	33.0	39.0
14	37.94592374312354	40.0	36.0	41.0	33.0	41.0
15	38.08940930160731	40.0	36.0	41.0	33.0	41.0
16	38.16355912680029	40.0	36.0	41.0	34.0	41.0
17	38.077230530091875	40.0	36.0	41.0	34.0	41.0
18	37.923760591584454	39.0	36.0	41.0	34.0	41.0
19	37.75251932244239	39.0	36.0	41.0	34.0	41.0
20	37.5155369398517	39.0	35.0	41.0	34.0	41.0
21	37.41843486317964	39.0	35.0	41.0	33.0	41.0
22	37.32538638555772	39.0	35.0	41.0	33.0	41.0
23	37.278969486616695	39.0	35.0	41.0	33.0	41.0
24	37.2165480611736	39.0	35.0	41.0	33.0	41.0
25	37.21993129235685	39.0	35.0	41.0	33.0	41.0
26	37.170009645398686	39.0	35.0	41.0	33.0	41.0
27	37.124332480183895	39.0	35.0	41.0	33.0	41.0
28	37.07508145425271	39.0	35.0	41.0	33.0	41.0
29	37.01599237481867	39.0	35.0	41.0	33.0	41.0
30	36.85177072811368	39.0	35.0	41.0	32.0	41.0
31	36.61562909011739	39.0	35.0	41.0	32.0	41.0
32	36.30723556797728	38.0	35.0	41.0	31.0	41.0
33	36.001696931953106	39.0	35.0	41.0	30.0	41.0
34	35.6284784826598	38.0	35.0	41.0	27.0	41.0
35	35.44933507507462	38.0	35.0	41.0	25.0	41.0
36	35.30650899857471	38.0	35.0	41.0	24.0	41.0
37	35.26620262425729	38.0	35.0	41.0	24.0	41.0
38	35.215474662474335	38.0	35.0	41.0	24.0	41.0
39	35.13172829848585	38.0	35.0	41.0	23.0	41.0
40	35.01742095709696	38.0	35.0	41.0	23.0	41.0
41	34.94076889776637	38.0	35.0	41.0	23.0	41.0
42	34.869932178417095	38.0	35.0	41.0	23.0	41.0
43	34.76429329100497	38.0	35.0	41.0	22.0	41.0
44	34.62143810109694	38.0	34.0	40.0	21.0	41.0
45	34.568063857096206	38.0	34.0	40.0	21.0	41.0
46	34.509044903001715	38.0	34.0	40.0	20.0	41.0
47	34.49254544856167	38.0	34.0	40.0	20.0	41.0
48	34.39725802327553	38.0	34.0	40.0	20.0	41.0
49	34.343602518436384	38.0	34.0	40.0	20.0	41.0
50	34.192382666636284	37.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	9.0
11	5.0
12	8.0
13	8.0
14	33.0
15	57.0
16	149.0
17	420.0
18	1066.0
19	2245.0
20	4045.0
21	6981.0
22	11229.0
23	17593.0
24	27263.0
25	45645.0
26	72867.0
27	93356.0
28	91743.0
29	82285.0
30	82982.0
31	90870.0
32	108904.0
33	139945.0
34	242836.0
35	493179.0
36	238118.0
37	281991.0
38	477571.0
39	1336663.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.636902637168454	25.792302414893914	28.602429830357433	17.968365117580195
2	32.843924284886086	27.597409666157812	29.11649666967927	10.442169379276823
3	26.405000417714113	25.286437961858905	37.22842886328597	11.080132757141014
4	27.363211284863304	25.497801304787004	36.280597559030596	10.85838985131909
5	21.38321599364062	32.77744445035151	34.319391808246436	11.519947747761432
6	19.16485024316025	40.07523917297668	32.41745082998529	8.34245975387778
7	74.63951271749615	7.401235927464576	15.728936449227483	2.2303149058117957
8	74.07091519897115	6.989217912593953	15.64410250957578	3.295764378859109
9	65.3309688182741	11.84781535517092	18.358155678253805	4.463060148301169
10	35.76526491935586	31.85943540241059	22.930479713017743	9.444819965215807
11	36.167713483558515	20.91307242656459	30.948793312523577	11.970420777353313
12	32.42691901662502	22.49486717956897	32.43572898708124	12.642484816724767
13	16.97288908804148	39.25302589574362	32.11467138557038	11.65941363064452
14	12.092773039465124	38.73723756794183	34.716346798917485	14.453642593675555
15	14.595893237335034	26.101841233193333	46.97514221266965	12.32712331680198
16	13.442445323753757	26.05893060122985	42.77526727374452	17.723356801271876
17	13.133665985666077	27.844190102960205	34.3600746315888	24.662069279784916
18	15.09917545765012	30.60588799692157	39.12880024910951	15.1661362963188
19	22.535170262805472	27.2213403813097	32.479753523355285	17.76373583252955
20	21.123043389104495	28.373826286622766	35.613900513155464	14.889229811117271
21	15.510889680436044	32.89465756303053	35.89703473609329	15.697418020440143
22	18.249474059953368	29.00548597872949	31.064284936722643	21.6807550245945
23	17.509208697567384	29.450237590726243	33.16371608604404	19.87683762566233
24	14.597690673836162	27.654927634193825	43.64783408901614	14.099547602953871
25	13.102653877019902	33.288017680699326	38.06332546005514	15.546002982225632
26	13.051794018840173	35.11535238616025	34.55852681091727	17.27432678408231
27	16.43029110876516	34.733586999724054	33.20422169733701	15.631900194173772
28	12.605042442285832	33.700921755816985	38.726275736885675	14.967760065011507
29	12.498006364444175	31.249572792380892	36.72010875756632	19.53231208560861
30	14.253570189895367	36.92278871007349	33.463812033710795	15.359829066320344
31	22.280491231800955	32.18527772925543	30.900414423035542	14.63381661590807
32	20.28343801502252	30.93550240881807	33.84036232269301	14.940697253466395
33	19.13930639203862	33.40907882645118	30.938768173728565	16.512846607781633
34	14.827635965944907	32.002597422324165	33.8304637639333	19.339302847797633
35	19.686891624705385	27.451386937446674	34.683486621756074	18.17823481609187
36	22.063634315341247	31.6919953317283	32.362996098803315	13.881374254127143
37	15.053657276959648	34.17822975289045	35.46327027115975	15.304842698990145
38	15.43258727060533	34.42599751396811	33.12538765135808	17.016027564068484
39	19.079763143437965	31.304128787591107	33.039338543367585	16.576769525603343
40	17.90492320389259	30.816188067553234	32.07457083039035	19.20431789816383
41	13.468090438903616	31.336786436696055	33.254195495269705	21.940927629130623
42	15.41524580577053	31.97738267929429	32.228694681360075	20.378676833575103
43	20.12582055507877	27.542119506742917	33.702719192318106	18.6293407458602
44	16.377785710126656	33.16070348120413	30.412448386990608	20.049062421678602
45	15.69450667962846	37.82753217031597	28.378332535879107	18.099628614176456
46	18.592303427534198	35.17889556387608	30.191844701486303	16.036956307103416
47	19.9664056586339	28.707820367740318	33.13569126623073	18.190082707395057
48	16.98501545542231	30.581128942018747	34.48857868341574	17.945276919143204
49	17.668218537899328	30.700898971410634	33.798236486948326	17.832646003741708
50	15.8356940509915	33.18282967137291	32.77336857321516	18.208107704420428
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15489.0
1	20003.0
2	24517.0
3	60851.5
4	97186.0
5	69730.0
6	42274.0
7	43109.5
8	43945.0
9	46132.5
10	48320.0
11	47448.5
12	46577.0
13	46088.5
14	45600.0
15	42256.5
16	38913.0
17	35983.5
18	33054.0
19	33252.5
20	33451.0
21	34439.0
22	35427.0
23	40644.0
24	45861.0
25	65604.0
26	85347.0
27	106687.0
28	128027.0
29	175809.0
30	223591.0
31	222458.0
32	221325.0
33	223734.5
34	226144.0
35	236710.5
36	247277.0
37	226260.0
38	205243.0
39	225944.5
40	246646.0
41	278132.0
42	309618.0
43	336903.5
44	364189.0
45	339995.0
46	315801.0
47	283623.5
48	251446.0
49	204243.5
50	157041.0
51	133477.5
52	109914.0
53	91098.5
54	72283.0
55	62670.5
56	53058.0
57	49032.5
58	45007.0
59	41304.0
60	37601.0
61	33989.5
62	30378.0
63	26818.0
64	23258.0
65	20039.5
66	16821.0
67	13640.0
68	10459.0
69	9023.5
70	7588.0
71	6517.0
72	5446.0
73	4287.0
74	3128.0
75	2458.5
76	1789.0
77	1149.5
78	510.0
79	402.0
80	294.0
81	219.0
82	144.0
83	93.5
84	43.0
85	31.5
86	20.0
87	14.0
88	8.0
89	5.0
90	2.0
91	3.5
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3950070.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.46134143473069
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.75706483187952	12.30601568855867
2	9.887097164048463	3.255097644315186
3	4.102629815432504	2.0260437051642186
4	2.322689514175336	1.5293834055883586
5	1.4343994886857796	1.1806069868529872
6	0.9597702442232767	0.9479463413432532
7	0.7091061778418989	0.8170987234852654
8	0.5456267001149101	0.7185397925197559
9	0.40744050083743694	0.6036315478760453
>10	3.2993568900752868	11.513180603591602
>50	0.750178997521724	8.867710204445743
>100	0.6538449143583867	20.941670454597023
>500	0.1034084642696279	11.97874878420636
>1k	0.06490200910616287	17.19839539728783
>5k	0.0017079476080569173	2.128083698066656
>10k+	7.763398218440534E-4	3.9878470221010076
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	57905	1.4659233886994407	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	41444	1.049196596516011	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	28273	0.7157594675537395	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	15898	0.40247388020971775	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	12504	0.31655135225451697	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9774	0.24743865298589648	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	9453	0.23931221472024547	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	9398	0.23791983433204983	No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	9073	0.22969213203816644	No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	8904	0.22541372684534705	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	8860	0.22429982253479053	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC	6831	0.1729336442139	No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	5458	0.1381747665231249	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT	5229	0.13237740090681938	No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	5209	0.13187108076565732	No Hit
TATGCAGTTTTCACCATTCTATATTGTAAAGAATATATATCCAGTATGTA	5072	0.12840278779869724	No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	4884	0.12364337847177392	No Hit
AGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4308	0.10906135840630672	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGC	4260	0.10784619006751778	No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4151	0.10508674529818458	No Hit
AGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4149	0.10503611328406838	No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	4109	0.10402347300174426	TruSeq Adapter, Index 13 (95% over 21bp)
AAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4034	0.10212477247238656	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11513720010025139	0.0
2	0.0	0.0	0.0	0.5138136792512538	0.0
3	0.0	0.0	0.0	0.7574549311784348	0.0
4	0.0	0.0	0.0	1.260281463366472	0.0
5	0.0	0.0	0.0	2.256466341102816	0.0
6	0.0	0.0	0.0	3.0336171257724547	0.0
7	0.0	0.0	0.0	3.5472535929743017	0.0
8	0.0	0.0	0.0	4.589665499598741	0.0
9	0.0	0.0	0.0	4.971506834056106	0.0
10	0.0	0.0	0.0	5.781543111894219	0.0
11	0.0	0.0	0.0	6.5246438670707105	0.0
12	0.0	0.0	0.0	7.23359332872582	0.0
13	0.0	0.0	0.0	7.484297746622212	0.0
14	0.0	0.0	0.0	7.571612654965608	0.0
15	0.0	0.0	0.0	7.729103534874065	0.0
16	0.0	0.0	0.0	8.068743085565572	0.0
17	0.0	0.0	0.0	8.472710610191719	0.0
18	0.0	0.0	0.0	9.019713574696144	0.0
19	0.0	0.0	0.0	9.27864569488642	0.0
20	0.0	0.0	0.0	9.54537514525059	0.0
21	0.0	0.0	0.0	9.910077542929619	0.0
22	0.0	0.0	0.0	10.27634953304625	0.0
23	0.0	0.0	0.0	10.69760789049308	0.0
24	0.0	0.0	0.0	11.024665385676709	0.0
25	0.0	0.0	0.0	11.304508527696978	0.0
26	0.0	0.0	0.0	11.578731516150347	0.0
27	0.0	0.0	0.0	11.831309318569039	0.0
28	0.0	0.0	0.0	12.085380765404157	0.0
29	0.0	0.0	0.0	12.369527628624303	0.0
30	0.0	0.0	0.0	12.781495011480809	0.0
31	0.0	0.0	0.0	13.147387261491568	0.0
32	0.0	0.0	0.0	13.449305961666502	0.0
33	0.0	0.0	0.0	13.733908513013693	0.0
34	2.531600705810277E-5	0.0	0.0	14.04111825866377	0.0
35	2.531600705810277E-5	0.0	0.0	14.46306014830117	0.0
36	7.59480211743083E-5	0.0	0.0	14.797130177439893	0.0
37	7.59480211743083E-5	0.0	0.0	15.09687170100783	0.0
38	7.59480211743083E-5	0.0	0.0	15.402739698283828	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGAAC	20	7.8588136E-4	44.000004	29
CAACCGT	40	8.3218765E-9	44.000004	31
CTACGCG	40	8.3218765E-9	44.000004	1
TACGAAC	20	7.8588136E-4	44.000004	12
CCGAGTA	20	7.8588136E-4	44.000004	19
ATACGCG	65	0.0	44.000004	1
TGTACGA	20	7.8588136E-4	44.000004	10
CAATACG	20	7.8588136E-4	44.000004	1
TAATGCG	130	0.0	44.000004	1
GCCGTAA	20	7.8588136E-4	44.000004	18
TACGTTA	20	7.8588136E-4	44.000004	43
TCGTTAG	290	0.0	44.0	1
GTCGATA	135	0.0	44.0	9
CGTGCAT	25	4.4448178E-5	44.0	26
ATTACGC	35	1.4472789E-7	44.0	42
TGCGAAC	25	4.4448178E-5	44.0	31
GTGTCGA	25	4.4448178E-5	44.0	38
CGTACTC	30	2.5292902E-6	44.0	7
TTAACGA	55	1.8189894E-12	44.0	38
CGGTATG	25	4.4448178E-5	44.0	1
>>END_MODULE