SRR1546194_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1546194_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4041879
Sequences flagged as poor quality	0
Sequence length	50
%GC	37

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59226	1.4653085854376147	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	42174	1.0434255948780258	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	29044	0.7185766817858724	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	16187	0.40048205302533796	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	12654	0.3130722122062536	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9878	0.2443912843506696	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	9715	0.2403585065262963	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	9563	0.23659787935264762	No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	9552	0.23632572870192306	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	9427	0.23323310767096195	No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	8961	0.22170381646753898	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC	6737	0.16667990308467917	No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	5751	0.14228530839245807	No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	5274	0.1304838665383105	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT	5030	0.12444707028587447	No Hit
TATGCAGTTTTCACCATTCTATATTGTAAAGAATATATATCCAGTATGTA	4982	0.12325950380998539	No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	4861	0.12026584665201508	No Hit
AGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4477	0.11076531484490257	No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4456	0.11024575451170113	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGC	4446	0.10999834482922421	No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	4191	0.1036893979260636	TruSeq Adapter, Index 13 (95% over 21bp)
AGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4134	0.10227916273594534	No Hit
GAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	4044	0.10005247559365335	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATA	150	0.0	44.000004	9
GGTACGA	40	8.3218765E-9	44.0	12
ACACGAA	30	2.5293048E-6	44.0	27
CAACCGT	40	8.3218765E-9	44.0	31
CTAACCG	20	7.858838E-4	44.0	1
TACGCGT	40	8.3218765E-9	44.0	22
TGCGCAA	30	2.5293048E-6	44.0	1
CCATACG	25	4.4448363E-5	44.0	42
CGTACTC	30	2.5293048E-6	44.0	7
AATTACG	120	0.0	44.0	1
TCGAGTA	20	7.858838E-4	44.0	30
ATACGTA	35	1.4472971E-7	44.0	26
AATACGT	35	1.4472971E-7	44.0	25
TACCGGA	40	8.3218765E-9	44.0	3
CCTTACG	130	0.0	43.999996	37
CGCATAC	45	4.802132E-10	43.999996	44
CTACGCG	45	4.802132E-10	43.999996	1
TTGCGTA	45	4.802132E-10	43.999996	11
TACGTTG	240	0.0	43.083332	1
GCGATAA	340	0.0	42.70588	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content