##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546194_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4041879
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.869452549173293	31.0	31.0	34.0	30.0	34.0
2	32.260255193191085	33.0	31.0	34.0	30.0	34.0
3	32.303278005106044	34.0	31.0	34.0	30.0	34.0
4	35.98383845731156	37.0	35.0	37.0	35.0	37.0
5	35.87481639108939	37.0	35.0	37.0	35.0	37.0
6	35.93914563003989	37.0	35.0	37.0	35.0	37.0
7	36.14460279488822	37.0	35.0	37.0	35.0	37.0
8	36.007803053975636	37.0	35.0	37.0	35.0	37.0
9	37.66629283063644	39.0	38.0	39.0	35.0	39.0
10	37.51816197367611	39.0	37.0	39.0	35.0	39.0
11	37.42186097109785	39.0	37.0	39.0	35.0	39.0
12	36.91955375210391	39.0	35.0	39.0	33.0	39.0
13	36.696974105360404	39.0	35.0	39.0	33.0	39.0
14	37.88757654546314	40.0	36.0	41.0	33.0	41.0
15	38.06174306553957	40.0	36.0	41.0	33.0	41.0
16	38.126337775079364	40.0	36.0	41.0	34.0	41.0
17	38.04164820371911	40.0	36.0	41.0	34.0	41.0
18	37.90598902144275	39.0	36.0	41.0	34.0	41.0
19	37.739081501450194	39.0	36.0	41.0	34.0	41.0
20	37.50056421778089	39.0	35.0	41.0	34.0	41.0
21	37.398936484738904	39.0	35.0	41.0	33.0	41.0
22	37.31555496836991	39.0	35.0	41.0	33.0	41.0
23	37.25672539925119	39.0	35.0	41.0	33.0	41.0
24	37.1989035297692	39.0	35.0	41.0	33.0	41.0
25	37.19703162811158	39.0	35.0	41.0	33.0	41.0
26	37.162455382756384	39.0	35.0	41.0	33.0	41.0
27	37.10827513639077	39.0	35.0	41.0	33.0	41.0
28	37.05195306440395	39.0	35.0	41.0	33.0	41.0
29	36.99235924677607	39.0	35.0	41.0	33.0	41.0
30	36.83305611078412	38.0	35.0	41.0	32.0	41.0
31	36.60686453008613	38.0	35.0	41.0	32.0	41.0
32	36.30854906839121	38.0	35.0	41.0	31.0	41.0
33	35.98445945561458	38.0	35.0	41.0	30.0	41.0
34	35.67529928530765	38.0	35.0	41.0	27.0	41.0
35	35.46915704304854	38.0	35.0	41.0	25.0	41.0
36	35.327486052897676	38.0	35.0	41.0	24.0	41.0
37	35.22190990873304	38.0	35.0	41.0	24.0	41.0
38	35.180896558259164	38.0	35.0	41.0	23.0	41.0
39	35.107753844189794	38.0	35.0	41.0	23.0	41.0
40	35.01102952364482	38.0	35.0	41.0	23.0	41.0
41	34.9495370841136	38.0	35.0	41.0	23.0	41.0
42	34.86192807850012	38.0	35.0	41.0	22.0	41.0
43	34.7815360133245	38.0	35.0	41.0	22.0	41.0
44	34.63155453193923	38.0	34.0	40.0	21.0	41.0
45	34.57385438802102	38.0	34.0	40.0	21.0	41.0
46	34.53121778262041	38.0	34.0	40.0	20.0	41.0
47	34.497836031212216	38.0	34.0	40.0	20.0	41.0
48	34.42451988295543	38.0	34.0	40.0	20.0	41.0
49	34.36216843700665	38.0	34.0	40.0	20.0	41.0
50	34.184614383557744	37.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	0.0
9	6.0
10	3.0
11	7.0
12	12.0
13	4.0
14	23.0
15	70.0
16	132.0
17	439.0
18	1100.0
19	2300.0
20	4152.0
21	7074.0
22	11581.0
23	18086.0
24	28120.0
25	46166.0
26	73914.0
27	94376.0
28	94183.0
29	86113.0
30	85963.0
31	94248.0
32	112602.0
33	145192.0
34	252658.0
35	497193.0
36	245540.0
37	290155.0
38	491117.0
39	1359348.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.70745982252314	25.801019773229232	28.70058702895361	17.790933375294017
2	32.90056431674476	27.552259728705387	29.087288362664985	10.459887591884863
3	26.386019967445833	25.282399596821183	37.201781646605454	11.129798789127532
4	27.419672879866024	25.48923409137186	36.168574071613726	10.922518957148396
5	21.413505946120605	32.76218808133544	34.26443493236685	11.559871040177105
6	19.188971268066165	40.04399933793169	32.35349202685187	8.413537367150278
7	74.65389240994102	7.39133952302877	15.697031009587374	2.2577370574428377
8	74.07945660916619	6.989150343194341	15.607592409371978	3.3238006382674987
9	65.36625663459989	11.820220249047535	18.31606042635121	4.497462690001358
10	35.8240808297329	31.757927439193505	22.9384155240669	9.479576207006692
11	36.1479648450634	20.916732044675264	30.892636815698836	12.042666294562505
12	32.37862885059152	22.489738064895064	32.39456203414303	12.737071050370385
13	17.021786154409867	39.16920818262991	32.12594934187788	11.683056321082349
14	12.108724679783833	38.675873275770996	34.74485505380047	14.470546990644698
15	14.60773565957813	26.02037319771324	47.002173988879925	12.369717153828702
16	13.518613496346624	25.95433955346016	42.757563994369946	17.769482955823268
17	13.144059978044865	27.786779366725227	34.35840112977158	24.710759525458332
18	15.14352606795008	30.514471116032915	39.11762326383348	15.224379552183526
19	22.528383457297956	27.191783821336564	32.46480164299822	17.815031078367262
20	21.14345827769708	28.35522785318412	35.56627994059199	14.935033928526806
21	15.547323410720606	32.80447039607074	35.90421682588717	15.743989367321486
22	18.20932788933068	28.969348167028258	31.06711012378154	21.754213819859526
23	17.514230386411864	29.380196685749375	33.128651303020206	19.976921624818555
24	14.620205107574966	27.587367162648857	43.5935860524276	14.19884167734858
25	13.142872411568977	33.234344719374334	38.059551015752824	15.563231853303872
26	13.085399142329596	35.06628971327444	34.53651136018669	17.311799784209274
27	16.42006106565783	34.684808723863334	33.19715904409806	15.697971166380784
28	12.593870326152762	33.618769884006916	38.71103019165096	15.076329598189355
29	12.518360891060818	31.177900179594687	36.729847677280794	19.573891252063707
30	14.250822451636974	36.861420146422	33.46854272480695	15.419214677134075
31	22.205860195221085	32.172115988628065	30.92371147181793	14.698312344332919
32	20.299568591736666	30.888999893366424	33.79059590848711	15.020835606409792
33	19.131572221731528	33.365397628182336	30.95429130857208	16.54873884151406
34	14.868653910718258	31.996405632132973	33.73478028412033	19.400160173028436
35	19.665333870707162	27.396614297459177	34.615459789865064	18.322592041968598
36	22.0692158275891	31.66289738015413	32.295078600819075	13.972808191437695
37	15.105152826197916	34.11158523053263	35.440224707370014	15.343037235899443
38	15.408501837882827	34.330864432111895	33.19268092884523	17.067952801160054
39	19.06474686649452	31.211127299951336	33.06590820754406	16.65821762601008
40	17.926266471608873	30.752701899289907	32.04811425577064	19.27291737333057
41	13.4910520577187	31.262217399382813	33.27009541849224	21.976635124406247
42	15.439576494001923	31.92755151749966	32.269719108365194	20.363152880133224
43	20.10552517777004	27.458961537443354	33.7548946913057	18.6806185934809
44	16.4117975822631	33.04883693945316	30.396234028777208	20.14313144950653
45	15.730827172213715	37.656891757521684	28.404833494520744	18.207447575743856
46	18.649321268647576	35.11045234159657	30.1329901266218	16.10723626313405
47	19.948741661984435	28.736956252277718	33.06593294851231	18.248369137225534
48	16.98687664821238	30.533719589329618	34.504818179861395	17.974585582596607
49	17.7487995063682	30.571028969447127	33.801902530976314	17.87826899320836
50	15.90421187769352	33.135554033161306	32.69937076295456	18.260863326190616
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16038.0
1	20542.5
2	25047.0
3	61989.5
4	98932.0
5	70663.0
6	42394.0
7	43539.0
8	44684.0
9	47131.5
10	49579.0
11	48333.0
12	47087.0
13	46452.5
14	45818.0
15	42644.0
16	39470.0
17	36418.0
18	33366.0
19	33782.5
20	34199.0
21	35123.5
22	36048.0
23	41413.5
24	46779.0
25	66589.0
26	86399.0
27	108230.5
28	130062.0
29	178615.0
30	227168.0
31	226260.5
32	225353.0
33	227430.5
34	229508.0
35	240406.5
36	251305.0
37	230458.5
38	209612.0
39	231383.0
40	253154.0
41	284874.5
42	316595.0
43	344576.5
44	372558.0
45	349022.0
46	325486.0
47	293466.0
48	261446.0
49	212823.5
50	164201.0
51	138973.0
52	113745.0
53	94439.5
54	75134.0
55	65029.0
56	54924.0
57	50475.0
58	46026.0
59	42135.5
60	38245.0
61	34481.0
62	30717.0
63	27242.5
64	23768.0
65	20381.5
66	16995.0
67	13862.5
68	10730.0
69	9254.0
70	7778.0
71	6669.5
72	5561.0
73	4299.5
74	3038.0
75	2448.5
76	1859.0
77	1197.0
78	535.0
79	417.0
80	299.0
81	229.5
82	160.0
83	100.0
84	40.0
85	27.0
86	14.0
87	13.0
88	12.0
89	8.5
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4041879.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.351031265563588
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.4948247608839	12.180672087878932
2	9.958903544124844	3.256766864414347
3	4.005185100134508	1.964667203900063
4	2.343376889222636	1.5326651513071388
5	1.5053298536746704	1.2306847751210397
6	1.0288316722960509	1.0093475304428878
7	0.7216813089158821	0.826016355209951
8	0.5536053325116912	0.724161448054511
9	0.44328525905622124	0.6523354017352555
>10	3.373979615597565	11.69191167956328
>50	0.7478964685462852	8.807385385979149
>100	0.6520000490909447	20.80681791894377
>500	0.10275183027245155	11.812541762251934
>1k	0.06605474803229026	17.524351255392965
>5k	0.0015290450933400528	1.9973169662304855
>10k+	7.645225466700264E-4	3.9823582135742908
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59226	1.4653085854376147	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	42174	1.0434255948780258	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	29044	0.7185766817858724	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	16187	0.40048205302533796	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	12654	0.3130722122062536	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9878	0.2443912843506696	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	9715	0.2403585065262963	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	9563	0.23659787935264762	No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	9552	0.23632572870192306	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	9427	0.23323310767096195	No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	8961	0.22170381646753898	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC	6737	0.16667990308467917	No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	5751	0.14228530839245807	No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	5274	0.1304838665383105	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT	5030	0.12444707028587447	No Hit
TATGCAGTTTTCACCATTCTATATTGTAAAGAATATATATCCAGTATGTA	4982	0.12325950380998539	No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	4861	0.12026584665201508	No Hit
AGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4477	0.11076531484490257	No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4456	0.11024575451170113	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGC	4446	0.10999834482922421	No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	4191	0.1036893979260636	TruSeq Adapter, Index 13 (95% over 21bp)
AGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4134	0.10227916273594534	No Hit
GAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	4044	0.10005247559365335	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11484757460577123	0.0
2	0.0	0.0	0.0	0.523617851994085	0.0
3	0.0	0.0	0.0	0.7702110825187988	0.0
4	0.0	0.0	0.0	1.2671581707418753	0.0
5	0.0	0.0	0.0	2.26488719726642	0.0
6	0.0	0.0	0.0	3.05756307895412	0.0
7	0.0	0.0	0.0	3.5815520454719203	0.0
8	0.0	0.0	0.0	4.63573996153769	0.0
9	0.0	0.0	0.0	5.0197692706783155	0.0
10	0.0	0.0	0.0	5.844905302706984	0.0
11	0.0	0.0	0.0	6.612963920988234	0.0
12	0.0	0.0	0.0	7.338641260661192	0.0
13	0.0	0.0	0.0	7.595922589468908	0.0
14	0.0	0.0	0.0	7.686177641636476	0.0
15	0.0	0.0	0.0	7.853253400213119	0.0
16	0.0	0.0	0.0	8.208533704249929	0.0
17	0.0	0.0	0.0	8.621608910113341	0.0
18	0.0	0.0	0.0	9.186098841652608	0.0
19	0.0	0.0	0.0	9.4570124439648	0.0
20	0.0	0.0	0.0	9.732181492815593	0.0
21	0.0	0.0	0.0	10.119179718145942	0.0
22	2.474096824768876E-5	0.0	0.0	10.50424814795297	0.0
23	2.474096824768876E-5	0.0	0.0	10.948744383490945	0.0
24	2.474096824768876E-5	0.0	0.0	11.285097846818275	0.0
25	2.474096824768876E-5	0.0	0.0	11.571796186872492	0.0
26	2.474096824768876E-5	0.0	0.0	11.851072236452403	0.0
27	2.474096824768876E-5	0.0	0.0	12.112237897275005	0.0
28	2.474096824768876E-5	0.0	0.0	12.382607198285747	0.0
29	2.474096824768876E-5	0.0	0.0	12.670888960307817	0.0
30	2.474096824768876E-5	0.0	0.0	13.088541245297051	0.0
31	2.474096824768876E-5	0.0	0.0	13.468661481454541	0.0
32	2.474096824768876E-5	0.0	0.0	13.78586543535816	0.0
33	2.474096824768876E-5	0.0	0.0	14.080456144283389	0.0
34	2.474096824768876E-5	0.0	0.0	14.39624986299689	0.0
35	2.474096824768876E-5	0.0	0.0	14.837183399107197	0.0
36	2.474096824768876E-5	0.0	0.0	15.18528882235218	0.0
37	2.474096824768876E-5	0.0	0.0	15.501181504938668	0.0
38	2.474096824768876E-5	0.0	0.0	15.809651897050852	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATA	150	0.0	44.000004	9
GGTACGA	40	8.3218765E-9	44.0	12
ACACGAA	30	2.5293048E-6	44.0	27
CAACCGT	40	8.3218765E-9	44.0	31
CTAACCG	20	7.858838E-4	44.0	1
TACGCGT	40	8.3218765E-9	44.0	22
TGCGCAA	30	2.5293048E-6	44.0	1
CCATACG	25	4.4448363E-5	44.0	42
CGTACTC	30	2.5293048E-6	44.0	7
AATTACG	120	0.0	44.0	1
TCGAGTA	20	7.858838E-4	44.0	30
ATACGTA	35	1.4472971E-7	44.0	26
AATACGT	35	1.4472971E-7	44.0	25
TACCGGA	40	8.3218765E-9	44.0	3
CCTTACG	130	0.0	43.999996	37
CGCATAC	45	4.802132E-10	43.999996	44
CTACGCG	45	4.802132E-10	43.999996	1
TTGCGTA	45	4.802132E-10	43.999996	11
TACGTTG	240	0.0	43.083332	1
GCGATAA	340	0.0	42.70588	9
>>END_MODULE