FastQCFastQC Report
Thu 26 May 2016
SRR1546193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546193_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3019110
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480371.5910980388260116No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA210840.6983515009390184No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA102240.3386428450768604No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA94530.3131055178512873No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA85170.28210300386537757No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA71360.23636104679855985No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA51190.16955327894644448No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA46820.15507881461755285No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG44510.14742755315308154No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38190.12649423174379204No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35440.11738558714323094No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA32490.10761449566262905No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA207.8585185E-444.00000432
GTCCGAT207.8585185E-444.00000416
TAAGCGT408.3200575E-944.00000438
CAATACG302.5290901E-644.0000041
CCGTACA254.4445667E-544.041
CGTTTTT266900.041.700261
ATATCGC850.041.41176639
GTATGCG1700.041.4117661
ACGTAGG3950.041.2151872
GCGTTAG1500.041.0666661
TCGATAT1500.041.06666615
GCGCTAA700.040.8571439
TACGGGA8050.040.7204974
TACGTGC1200.040.33333632
GGACTAA27200.039.9558838
GGGACTA29150.039.8490567
GGATGCC91300.039.734948
CCCTAGC21600.039.6203721
TTTACGA501.3496901E-939.60000232
ACGGGAT10200.039.4705855