##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546193_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3019110
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91522203563302	31.0	31.0	34.0	30.0	34.0
2	32.271165012205586	33.0	31.0	34.0	30.0	34.0
3	32.22789232588412	34.0	31.0	34.0	30.0	34.0
4	35.95974939634528	37.0	35.0	37.0	35.0	37.0
5	35.944441242617856	37.0	35.0	37.0	35.0	37.0
6	35.99417278601972	37.0	35.0	37.0	35.0	37.0
7	36.18556031413231	37.0	35.0	37.0	35.0	37.0
8	36.06354024861631	37.0	35.0	37.0	35.0	37.0
9	37.74388114378078	39.0	38.0	39.0	35.0	39.0
10	37.51331485106538	39.0	37.0	39.0	35.0	39.0
11	37.413587116733076	39.0	37.0	39.0	35.0	39.0
12	37.10855086432757	39.0	37.0	39.0	34.0	39.0
13	37.02466488468456	39.0	37.0	39.0	33.0	39.0
14	38.28296650337351	40.0	37.0	41.0	33.0	41.0
15	38.3503128405391	40.0	37.0	41.0	33.0	41.0
16	38.384667666961455	40.0	37.0	41.0	34.0	41.0
17	38.26866030055215	40.0	37.0	41.0	34.0	41.0
18	38.09586500657478	40.0	36.0	41.0	34.0	41.0
19	37.93483311306974	40.0	36.0	41.0	34.0	41.0
20	37.71329795866994	40.0	35.0	41.0	34.0	41.0
21	37.65270758601045	40.0	35.0	41.0	33.0	41.0
22	37.54642593347046	39.0	35.0	41.0	33.0	41.0
23	37.48313840833888	39.0	35.0	41.0	33.0	41.0
24	37.38944854609471	39.0	35.0	41.0	33.0	41.0
25	37.35768488064363	39.0	35.0	41.0	33.0	41.0
26	37.304351613554985	39.0	35.0	41.0	33.0	41.0
27	37.24928505420472	39.0	35.0	41.0	33.0	41.0
28	37.180236559780866	39.0	35.0	41.0	33.0	41.0
29	37.1048954824435	39.0	35.0	41.0	33.0	41.0
30	36.9171875155261	39.0	35.0	41.0	32.0	41.0
31	36.70012189022593	39.0	35.0	41.0	31.0	41.0
32	36.442573473639584	39.0	35.0	41.0	30.0	41.0
33	36.132751704972655	39.0	35.0	41.0	30.0	41.0
34	35.74211042327043	39.0	35.0	41.0	26.0	41.0
35	35.54520040674238	39.0	35.0	41.0	24.0	41.0
36	35.38779739724621	39.0	35.0	41.0	23.0	41.0
37	35.33393218531289	39.0	35.0	41.0	23.0	41.0
38	35.269718890666454	39.0	35.0	41.0	23.0	41.0
39	35.177705019028785	39.0	35.0	41.0	23.0	41.0
40	35.06440308567757	39.0	35.0	41.0	23.0	41.0
41	34.9649048891892	39.0	35.0	41.0	22.0	41.0
42	34.91799338215567	38.0	35.0	41.0	22.0	41.0
43	34.785749111493125	38.0	35.0	41.0	21.0	41.0
44	34.632189618794946	38.0	34.0	41.0	20.0	41.0
45	34.613046228855524	38.0	34.0	40.0	20.0	41.0
46	34.542112410611075	38.0	34.0	40.0	20.0	41.0
47	34.52020198005373	38.0	34.0	40.0	20.0	41.0
48	34.41615972919172	38.0	34.0	40.0	20.0	41.0
49	34.314498974863454	38.0	34.0	40.0	20.0	41.0
50	34.23797079271706	38.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	8.0
11	11.0
12	17.0
13	16.0
14	38.0
15	71.0
16	157.0
17	368.0
18	948.0
19	1938.0
20	3581.0
21	5753.0
22	9119.0
23	13934.0
24	21600.0
25	35601.0
26	55742.0
27	70253.0
28	70659.0
29	65219.0
30	64403.0
31	68829.0
32	79881.0
33	100562.0
34	169753.0
35	293051.0
36	187897.0
37	237035.0
38	408249.0
39	1054416.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.17011635879448	24.490230564636597	27.11385143303821	18.22580164353071
2	34.609835348827964	25.985969375080735	28.332422468873276	11.071772807218021
3	28.281248447390123	23.697314771571754	37.27124881173591	10.750187969302212
4	25.445412720967436	26.209677686470517	36.25767196292947	12.087237629632575
5	21.014139928654473	31.912914733149833	35.001142720868074	12.071802617327624
6	18.933493645478304	40.08456134423721	32.880782747233454	8.101162263051032
7	77.18678683453071	5.915451904700392	15.066923696056122	1.8308375647127797
8	77.1833421107545	5.442663566415268	15.027806207789713	2.3461881150405253
9	68.95512253611163	10.163624379370079	17.254919496142904	3.626333588375382
10	32.822421177101866	34.565252673801226	23.54561443604241	9.06671171305451
11	31.37162938746849	22.31717294169474	33.856467634501556	12.454730036335212
12	30.575566971723454	22.65223857361938	33.414714932546346	13.357479522110822
13	18.738104938210267	31.072667110506075	33.078291284517626	17.110936666766033
14	14.183352047457696	33.06004087297249	35.29927693923043	17.457330140339373
15	16.30583185110844	27.302251325721823	42.55075171159713	13.841165111572614
16	17.070991119899574	24.98762880451524	40.170381337546495	17.770998738038692
17	16.061620808781395	28.46381880752937	34.77147238755792	20.703087996131313
18	16.9772548863738	30.767245976463265	36.50767941545687	15.747819721706065
19	21.963591919472957	26.965860800037095	33.049276111171835	18.02127116931811
20	21.14156158603032	27.29655428255347	36.94979646319611	14.612087668220106
21	18.16283606758283	30.605045857885273	35.96029955847915	15.271818516052743
22	20.707526390227585	27.182017216994414	32.931393688868575	19.17906270390943
23	19.88589352491297	28.419732967662654	34.44462109694579	17.249752410478585
24	16.635233562208732	26.88229312611995	41.90990059984565	14.572572711825671
25	15.789189529364615	32.12132052161068	35.92144704896476	16.16804290005995
26	14.950995492048982	31.726369691730344	34.38877682495835	18.933857991262325
27	17.064929730947203	31.70093173153678	34.683201340792486	16.550937196723538
28	14.271424360159118	31.234469760956042	38.63174909161972	15.862356787265123
29	15.025090175581546	27.617874141717262	37.01703482152025	20.340000861180943
30	15.865602776977322	33.12837226864871	34.277353259735484	16.728671694638486
31	20.509620384815392	32.270039846179834	31.78065721354969	15.439682555455084
32	19.71345860203835	30.166936613770286	32.36573029800173	17.753874486189638
33	18.69199863535943	33.626234221343374	30.33685423850075	17.34491290479645
34	15.802173488213414	31.223108796963345	34.57489127590581	18.39982643891743
35	21.148186054830727	28.640857736220276	32.77187648015475	17.439079728794248
36	19.408699914875577	34.6314973618053	30.30893210250703	15.650870620812093
37	16.52162392228173	34.60261467783552	33.76213519878375	15.113626201098999
38	16.261414787801705	33.18136801905197	33.51557247003256	17.041644723113766
39	19.259152531706363	30.41376432127349	33.79330332448966	16.53377982253048
40	18.60730480174621	31.348509991355066	31.816329978039885	18.227855228858836
41	14.661870551255172	31.314195242968957	34.274173514711286	19.749760691064587
42	16.88706274365624	30.611570959653672	30.86555309346132	21.635813203228764
43	19.16359456926048	30.501969123350918	31.221088333979218	19.113347973409383
44	16.630265210608425	31.98104739476203	30.988867580180912	20.399819814448627
45	16.5178479750655	36.390691296441666	28.446661433336313	18.64479929515652
46	17.493731596397613	35.92376561304491	29.451692717390227	17.130810073167257
47	18.769273063916188	30.44980143154771	32.91748230438772	17.86344320014839
48	16.919721374842254	30.313999821139344	35.32047523939174	17.44580356462666
49	17.0860286640765	32.6412419554107	32.36119253687345	17.91153684363935
50	15.893789891723056	32.26825123960372	33.20846209644564	18.629496772227576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5382.0
1	12690.0
2	19998.0
3	54595.0
4	89192.0
5	62700.5
6	36209.0
7	35173.5
8	34138.0
9	34088.5
10	34039.0
11	33747.0
12	33455.0
13	32113.0
14	30771.0
15	29060.5
16	27350.0
17	27896.0
18	28442.0
19	26924.0
20	25406.0
21	29053.0
22	32700.0
23	36112.5
24	39525.0
25	53152.5
26	66780.0
27	82632.0
28	98484.0
29	118496.0
30	138508.0
31	153901.5
32	169295.0
33	182111.5
34	194928.0
35	198134.5
36	201341.0
37	188006.5
38	174672.0
39	175742.5
40	176813.0
41	188539.5
42	200266.0
43	197234.0
44	194202.0
45	186417.5
46	178633.0
47	160199.5
48	141766.0
49	127595.0
50	113424.0
51	100575.5
52	87727.0
53	79620.0
54	71513.0
55	67881.0
56	64249.0
57	63759.0
58	63269.0
59	63999.0
60	64729.0
61	60088.0
62	55447.0
63	48995.0
64	42543.0
65	35421.0
66	28299.0
67	23564.0
68	18829.0
69	16104.0
70	13379.0
71	11923.0
72	10467.0
73	8041.0
74	5615.0
75	4583.5
76	3552.0
77	2737.5
78	1923.0
79	1441.0
80	959.0
81	741.0
82	523.0
83	341.0
84	159.0
85	146.0
86	133.0
87	79.5
88	26.0
89	22.0
90	18.0
91	15.0
92	12.0
93	11.5
94	11.0
95	10.0
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3019110.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.425316991913743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.34674877203548	15.024851131907047
2	8.699376530937045	3.37976293490934
3	3.1808592772850126	1.8536759930379312
4	1.6316652891550265	1.2678246186615596
5	1.0580227591538685	1.0276213740611553
6	0.7772871507325164	0.9059429578032343
7	0.5853733336529117	0.795974379537477
8	0.4817605416419591	0.7486680988472894
9	0.3942739881893821	0.6893007482020322
>10	4.374006460602821	19.306108406487894
>50	0.8075846621525427	10.66373560380937
>100	0.5450105885600445	22.622475682057967
>500	0.1014891247239199	13.503318675292958
>1k	0.015335368591560053	4.5432911955686
>5k	6.892300490588788E-4	1.0116694518521918
>10k+	5.16922536794159E-4	2.6557787479640083
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48037	1.5910980388260116	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	21084	0.6983515009390184	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	10224	0.3386428450768604	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	9453	0.3131055178512873	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	8517	0.28210300386537757	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	7136	0.23636104679855985	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	5119	0.16955327894644448	No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4682	0.15507881461755285	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4451	0.14742755315308154	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3819	0.12649423174379204	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3544	0.11738558714323094	No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3249	0.10761449566262905	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05153836726717476	0.0
2	0.0	0.0	0.0	0.19787288306818898	0.0
3	0.0	0.0	0.0	0.2936627019220896	0.0
4	0.0	0.0	0.0	0.4671575398047769	0.0
5	0.0	0.0	0.0	0.8642944443892405	0.0
6	0.0	0.0	0.0	1.2245993024434354	0.0
7	0.0	0.0	0.0	1.4633782803541442	0.0
8	0.0	0.0	0.0	1.9855851558903121	0.0
9	0.0	0.0	0.0	2.1812719642543663	0.0
10	0.0	0.0	0.0	2.534852986476147	0.0
11	0.0	0.0	0.0	2.956102957494096	0.0
12	0.0	0.0	0.0	3.3181964221244007	0.0
13	0.0	0.0	0.0	3.451050143916585	0.0
14	0.0	0.0	0.0	3.506463825431998	0.0
15	0.0	0.0	0.0	3.595894154237507	0.0
16	0.0	0.0	0.0	3.7825054403450022	0.0
17	0.0	0.0	0.0	4.006048140014773	0.0
18	0.0	0.0	0.0	4.2746041051833155	0.0
19	0.0	0.0	0.0	4.444886075697805	0.0
20	0.0	0.0	0.0	4.627357068805045	0.0
21	0.0	0.0	0.0	4.8782588246205005	0.0
22	0.0	0.0	0.0	5.150093901845246	0.0
23	0.0	0.0	0.0	5.421862734381987	0.0
24	0.0	0.0	0.0	5.647227162971869	0.0
25	0.0	0.0	0.0	5.849207216696311	0.0
26	0.0	0.0	0.0	6.062912580197476	0.0
27	0.0	0.0	0.0	6.25455846259328	0.0
28	0.0	0.0	0.0	6.464951591694241	0.0
29	0.0	0.0	0.0	6.690349142628126	0.0
30	0.0	0.0	0.0	6.969007422717291	0.0
31	0.0	0.0	0.0	7.2484937614065075	0.0
32	0.0	0.0	0.0	7.503270831470202	0.0
33	0.0	0.0	0.0	7.74009559108479	0.0
34	0.0	0.0	0.0	7.992951565196366	0.0
35	0.0	0.0	0.0	8.293404347638873	0.0
36	3.312234400204034E-5	0.0	0.0	8.562357780935441	0.0
37	3.312234400204034E-5	0.0	0.0	8.831642437672029	0.0
38	3.312234400204034E-5	0.0	0.0	9.106922238672986	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTA	20	7.8585185E-4	44.000004	32
GTCCGAT	20	7.8585185E-4	44.000004	16
TAAGCGT	40	8.3200575E-9	44.000004	38
CAATACG	30	2.5290901E-6	44.000004	1
CCGTACA	25	4.4445667E-5	44.0	41
CGTTTTT	26690	0.0	41.70026	1
ATATCGC	85	0.0	41.411766	39
GTATGCG	170	0.0	41.411766	1
ACGTAGG	395	0.0	41.215187	2
GCGTTAG	150	0.0	41.066666	1
TCGATAT	150	0.0	41.066666	15
GCGCTAA	70	0.0	40.857143	9
TACGGGA	805	0.0	40.720497	4
TACGTGC	120	0.0	40.333336	32
GGACTAA	2720	0.0	39.955883	8
GGGACTA	2915	0.0	39.849056	7
GGATGCC	9130	0.0	39.73494	8
CCCTAGC	2160	0.0	39.62037	21
TTTACGA	50	1.3496901E-9	39.600002	32
ACGGGAT	1020	0.0	39.470585	5
>>END_MODULE