FastQCFastQC Report
Thu 26 May 2016
SRR1546192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546192_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3085197
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT491321.592507706963283No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA217300.7043310362352874No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA105580.3422147759122027No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA97670.31657621863368857No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA85990.2787180202755286No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA72010.2334048684735529No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA51490.16689371861829244No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA48580.15746158186981252No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG46170.14965008717433603No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39450.12786865798197003No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38190.1237846400084014No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA33420.10832371482274875No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG254.4445893E-544.01
AACGCGT207.858547E-444.020
CGTTTTT265700.041.7561151
ACGTAGG4250.040.8941152
CCCTAGC24250.040.643321
GCGAATG1300.040.6153831
TCGATAT1200.040.33333615
CATACGG2350.040.2553182
GGACTAA30900.040.226548
TCACGAC2200.040.025
CTAACGT557.8216544E-1140.020
AAGGGCG8800.040.05
AATCGAC557.8216544E-1140.016
CCTACAC24500.039.86938528
ATCCTAG25000.039.86414
CCCTACA24250.039.82680527
GGGACTA33600.039.547627
CCATACG1950.039.487181
GGATGCC94600.039.4186068
CGACGAA2250.039.1111138
Produced by FastQC (version 0.11.5)