##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546192_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3085197
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84767714995185	31.0	31.0	34.0	30.0	34.0
2	32.23622122023326	33.0	31.0	34.0	30.0	34.0
3	32.30616683472725	34.0	31.0	34.0	30.0	34.0
4	35.98713437099803	37.0	35.0	37.0	35.0	37.0
5	35.90011853375976	37.0	35.0	37.0	35.0	37.0
6	35.97797320560081	37.0	35.0	37.0	35.0	37.0
7	36.164520774524284	37.0	35.0	37.0	35.0	37.0
8	36.04269775965684	37.0	35.0	37.0	35.0	37.0
9	37.71245790787428	39.0	38.0	39.0	35.0	39.0
10	37.50783272510637	39.0	37.0	39.0	35.0	39.0
11	37.4087667659472	39.0	37.0	39.0	35.0	39.0
12	37.094340491061025	39.0	37.0	39.0	34.0	39.0
13	36.98637040033424	39.0	37.0	39.0	33.0	39.0
14	38.23059273038318	40.0	37.0	41.0	33.0	41.0
15	38.325087506567655	40.0	37.0	41.0	33.0	41.0
16	38.34207799372293	40.0	37.0	41.0	34.0	41.0
17	38.23456913772443	40.0	37.0	41.0	34.0	41.0
18	38.07489829660796	40.0	36.0	41.0	34.0	41.0
19	37.913473920790146	40.0	36.0	41.0	34.0	41.0
20	37.69665535134385	40.0	35.0	41.0	34.0	41.0
21	37.634995431410054	40.0	35.0	41.0	33.0	41.0
22	37.537191304153346	39.0	35.0	41.0	33.0	41.0
23	37.46325793782374	39.0	35.0	41.0	33.0	41.0
24	37.37982469190784	39.0	35.0	41.0	33.0	41.0
25	37.334591599823284	39.0	35.0	41.0	33.0	41.0
26	37.298799720082705	39.0	35.0	41.0	33.0	41.0
27	37.237107387307844	39.0	35.0	41.0	33.0	41.0
28	37.15385694981552	39.0	35.0	41.0	33.0	41.0
29	37.08254286517198	39.0	35.0	41.0	33.0	41.0
30	36.898055456426285	39.0	35.0	41.0	32.0	41.0
31	36.6964090137518	39.0	35.0	41.0	31.0	41.0
32	36.445954018495414	39.0	35.0	41.0	30.0	41.0
33	36.12206092512083	39.0	35.0	41.0	30.0	41.0
34	35.80768294536783	39.0	35.0	41.0	27.0	41.0
35	35.57913254809984	39.0	35.0	41.0	25.0	41.0
36	35.422783374935214	39.0	35.0	41.0	24.0	41.0
37	35.29877800347919	39.0	35.0	41.0	23.0	41.0
38	35.238746828808665	39.0	35.0	41.0	23.0	41.0
39	35.15374350487181	39.0	35.0	41.0	23.0	41.0
40	35.057417079039034	39.0	35.0	41.0	23.0	41.0
41	34.97722025530298	39.0	35.0	41.0	22.0	41.0
42	34.913816848648565	38.0	35.0	41.0	22.0	41.0
43	34.81436452842396	38.0	35.0	41.0	21.0	41.0
44	34.66840334669067	38.0	34.0	41.0	20.0	41.0
45	34.637910642334994	38.0	34.0	40.0	20.0	41.0
46	34.57063843897164	38.0	34.0	40.0	20.0	41.0
47	34.52380155951143	38.0	34.0	40.0	20.0	41.0
48	34.443255973605574	38.0	34.0	40.0	20.0	41.0
49	34.34214962610167	38.0	34.0	40.0	20.0	41.0
50	34.24012761583782	38.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	5.0
11	12.0
12	9.0
13	19.0
14	44.0
15	62.0
16	164.0
17	420.0
18	983.0
19	2060.0
20	3621.0
21	6090.0
22	9446.0
23	14383.0
24	22036.0
25	35476.0
26	55972.0
27	71191.0
28	72127.0
29	67392.0
30	66323.0
31	71357.0
32	82196.0
33	104609.0
34	176425.0
35	296338.0
36	193992.0
37	243042.0
38	418131.0
39	1071267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.407815124933673	24.56115444167747	27.07480267872684	17.95622775466202
2	34.601550565490626	25.960060248988963	28.329212040592544	11.10917714492786
3	28.320525399188444	23.669509596956047	37.21217802299173	10.797786980863783
4	25.502131630492315	26.2248083347676	36.14044743334056	12.132612601399522
5	21.069578377004774	31.96713208265145	34.824064719368	12.139224820975777
6	18.962451992530784	40.09769878552326	32.737682553172455	8.2021666687735
7	77.26436917966664	5.907856127177616	14.973111927698621	1.8546627654571168
8	77.19844146095048	5.481497615873476	14.938332949241168	2.3817279739348898
9	68.95747662142806	10.212508309842127	17.192581219286808	3.637433849443002
10	32.84927996494227	34.57318932956307	23.427807041171114	9.149723664323542
11	31.43121816856428	22.310017804373594	33.78607589726037	12.47268812980176
12	30.544467662842923	22.679621430981555	33.34185791053213	13.43405299564339
13	18.786741981144154	31.174411228845354	32.99277161231519	17.046075177695297
14	14.219513373052028	33.10148428123066	35.204818363300625	17.474183982416683
15	16.400703099348274	27.246525910663077	42.51034860982945	13.842422380159192
16	17.106557539113386	25.0230698396245	40.0881694102516	17.782203211010515
17	16.080237339787377	28.484599200634513	34.724816600042075	20.710346859536035
18	17.026919188628796	30.686824860778746	36.48749172257072	15.798764228021744
19	22.082058293198134	26.94265552572494	33.048262396210035	17.9270237848669
20	21.234948692093244	27.23884406733184	36.86467995398673	14.661527286588182
21	18.213715364043203	30.64796186434772	35.83787356204482	15.30044920956425
22	20.661954487833352	27.20095345613262	32.86769045866439	19.269401597369633
23	19.933443472167255	28.370343935897775	34.378096439222524	17.318116152712452
24	16.70590241076988	26.88606270523406	41.77525778742816	14.632777096567901
25	15.811696951604711	32.0212291143807	35.85595992735634	16.311114006658244
26	14.93078075727417	31.72646673778044	34.408467271295805	18.934285233649586
27	17.08380372468922	31.69878617151514	34.6439465615972	16.57346354219844
28	14.233418481866797	31.301469565800822	38.5736794117199	15.89143254061248
29	15.066525735633737	27.59470464933034	36.96130263318679	20.377466981849132
30	15.85428742475764	33.078892531011796	34.27888073273765	16.787939311492913
31	20.543291076712443	32.273822384761814	31.711005812594788	15.471880725930953
32	19.708206639640842	30.240888993474325	32.29524727270252	17.755657094182318
33	18.725708601428046	33.59733592376759	30.27015778895156	17.406797685852798
34	15.822490427677712	31.29242638314506	34.364094091884574	18.520989097292652
35	21.031493288759194	28.683970585994995	32.74115072716589	17.543385398079927
36	19.40812855710673	34.654286257895365	30.210453335718917	15.727131849278992
37	16.537906655555545	34.622197545245896	33.65866101905324	15.181234780145319
38	16.23708307767705	33.24601313951751	33.42340861863926	17.09349516416618
39	19.247911883746806	30.39744301579445	33.76432688090906	16.590318219549676
40	18.610643015664802	31.345388965437216	31.79210274092708	18.251865277970904
41	14.666486451270371	31.27696545795941	34.225367132147476	19.831180958622742
42	16.92313327155446	30.57655637549239	30.8949801260665	21.605330226886647
43	19.08866759561869	30.429596554126043	31.301048198867043	19.180687651388226
44	16.63138528917278	31.893036327988135	30.96418154172975	20.511396841109335
45	16.523515354124875	36.372717852377015	28.397538309547173	18.706228483950945
46	17.46027887360191	35.937154094211806	29.395075906011837	17.20749112617444
47	18.724217610739284	30.46563963338484	32.82091872901471	17.98922402686117
48	16.827547803268317	30.284224961971635	35.32892713171963	17.55930010304042
49	17.082345146841515	32.574451485593954	32.347723662378776	17.995479705185762
50	15.89642411813573	32.294469364517084	33.092279034369604	18.716827482977585
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5369.0
1	13026.0
2	20683.0
3	55800.0
4	90917.0
5	63446.5
6	35976.0
7	35130.0
8	34284.0
9	34468.5
10	34653.0
11	34097.5
12	33542.0
13	32382.0
14	31222.0
15	29680.0
16	28138.0
17	28435.5
18	28733.0
19	27248.5
20	25764.0
21	29542.0
22	33320.0
23	36641.5
24	39963.0
25	53808.5
26	67654.0
27	84003.0
28	100352.0
29	120782.5
30	141213.0
31	156558.0
32	171903.0
33	185155.5
34	198408.0
35	201222.0
36	204036.0
37	190927.0
38	177818.0
39	178754.5
40	179691.0
41	192916.5
42	206142.0
43	202673.0
44	199204.0
45	192201.0
46	185198.0
47	166272.5
48	147347.0
49	132229.5
50	117112.0
51	104174.0
52	91236.0
53	82621.5
54	74007.0
55	70075.0
56	66143.0
57	65165.5
58	64188.0
59	65047.0
60	65906.0
61	61324.0
62	56742.0
63	49977.5
64	43213.0
65	36232.0
66	29251.0
67	23951.5
68	18652.0
69	16210.0
70	13768.0
71	12172.5
72	10577.0
73	8095.0
74	5613.0
75	4573.0
76	3533.0
77	2719.5
78	1906.0
79	1411.0
80	916.0
81	733.0
82	550.0
83	350.0
84	150.0
85	145.0
86	140.0
87	85.5
88	31.0
89	22.5
90	14.0
91	10.0
92	6.0
93	7.0
94	8.0
95	6.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3085197.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.304372200787615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.09946173409912	14.883567057954316
2	8.657643091953265	3.342607292572871
3	3.308845534800432	1.9162555727610504
4	1.7659201675329004	1.3635992076372931
5	1.0763006246455158	1.0388653929048621
6	0.7822081834188107	0.90600227467312
7	0.5958516277839144	0.8051779119430093
8	0.46221132568929235	0.7138159573220451
9	0.3868668423572709	0.6721399365307367
>10	4.378271055426994	19.11669267062437
>50	0.8254631513188436	10.881511497748312
>100	0.5434574622044462	22.546372883777423
>500	0.0993046773969255	13.181115649025136
>1k	0.017004225581665325	4.95134633860703
>5k	6.80169023266613E-4	1.008270821256272
>10k+	5.101267674499598E-4	2.6726595346622495
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49132	1.592507706963283	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	21730	0.7043310362352874	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	10558	0.3422147759122027	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	9767	0.31657621863368857	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	8599	0.2787180202755286	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	7201	0.2334048684735529	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	5149	0.16689371861829244	No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4858	0.15746158186981252	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4617	0.14965008717433603	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3945	0.12786865798197003	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3819	0.1237846400084014	No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3342	0.10832371482274875	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05562043525907746	0.0
2	0.0	0.0	0.0	0.2043305500426715	0.0
3	0.0	0.0	0.0	0.3000132568519936	0.0
4	0.0	0.0	0.0	0.4769225433578472	0.0
5	0.0	0.0	0.0	0.8648070123236863	0.0
6	0.0	0.0	0.0	1.2326279326733431	0.0
7	0.0	0.0	0.0	1.48214198315375	0.0
8	0.0	0.0	0.0	2.0241819242012746	0.0
9	3.241284106006845E-5	0.0	0.0	2.224655346157798	0.0
10	3.241284106006845E-5	0.0	0.0	2.589170156719328	0.0
11	3.241284106006845E-5	0.0	0.0	3.0231456856725845	0.0
12	3.241284106006845E-5	0.0	0.0	3.3969629816183535	0.0
13	3.241284106006845E-5	0.0	0.0	3.5357871798786267	0.0
14	3.241284106006845E-5	0.0	0.0	3.590791771157563	0.0
15	3.241284106006845E-5	0.0	0.0	3.681353249079394	0.0
16	3.241284106006845E-5	0.0	0.0	3.8798819005723137	0.0
17	3.241284106006845E-5	0.0	0.0	4.112476448019365	0.0
18	3.241284106006845E-5	0.0	0.0	4.400010761063232	0.0
19	3.241284106006845E-5	0.0	0.0	4.576693157681665	0.0
20	3.241284106006845E-5	0.0	0.0	4.776064543042146	0.0
21	3.241284106006845E-5	0.0	0.0	5.039872656430044	0.0
22	3.241284106006845E-5	0.0	0.0	5.323614667069882	0.0
23	3.241284106006845E-5	0.0	0.0	5.610079356358767	0.0
24	3.241284106006845E-5	0.0	0.0	5.853175664309281	0.0
25	3.241284106006845E-5	0.0	0.0	6.071767864418383	0.0
26	3.241284106006845E-5	0.0	0.0	6.295708183302395	0.0
27	3.241284106006845E-5	0.0	0.0	6.497478118901322	0.0
28	3.241284106006845E-5	0.0	0.0	6.716426860262084	0.0
29	3.241284106006845E-5	0.0	0.0	6.94668768315281	0.0
30	3.241284106006845E-5	0.0	0.0	7.232439289938373	0.0
31	3.241284106006845E-5	0.0	0.0	7.522696281631286	0.0
32	3.241284106006845E-5	0.0	0.0	7.792241467886815	0.0
33	3.241284106006845E-5	0.0	0.0	8.037088069254573	0.0
34	3.241284106006845E-5	0.0	0.0	8.295515651026498	0.0
35	3.241284106006845E-5	0.0	0.0	8.601557696315664	0.0
36	3.241284106006845E-5	0.0	0.0	8.876062047253384	0.0
37	3.241284106006845E-5	0.0	0.0	9.155201434462693	0.0
38	3.241284106006845E-5	0.0	0.0	9.445977031612568	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAACG	25	4.4445893E-5	44.0	1
AACGCGT	20	7.858547E-4	44.0	20
CGTTTTT	26570	0.0	41.756115	1
ACGTAGG	425	0.0	40.894115	2
CCCTAGC	2425	0.0	40.6433	21
GCGAATG	130	0.0	40.615383	1
TCGATAT	120	0.0	40.333336	15
CATACGG	235	0.0	40.255318	2
GGACTAA	3090	0.0	40.22654	8
TCACGAC	220	0.0	40.0	25
CTAACGT	55	7.8216544E-11	40.0	20
AAGGGCG	880	0.0	40.0	5
AATCGAC	55	7.8216544E-11	40.0	16
CCTACAC	2450	0.0	39.869385	28
ATCCTAG	2500	0.0	39.864	14
CCCTACA	2425	0.0	39.826805	27
GGGACTA	3360	0.0	39.54762	7
CCATACG	195	0.0	39.48718	1
GGATGCC	9460	0.0	39.418606	8
CGACGAA	225	0.0	39.11111	38
>>END_MODULE