FastQCFastQC Report
Thu 26 May 2016
SRR1546191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546191_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1599612
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177481.1095190583716552No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA99310.6208380532279077No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA92550.578577805117741No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA63540.3972213261715966No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA44000.27506670367564134No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA40250.25162351870328553No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG33150.20723775515562523No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA20290.1268432594904264No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA18740.1171534097018527No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA18420.11515292458421167No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAA18110.11321495462649693No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16960.10602571123497448No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16100.10064940748131422No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTG650.044.0000047
ATTTACG351.4465695E-744.01
GGTCGTA351.4465695E-744.023
CATGACG302.5283407E-644.01
CGTCTAC254.4436245E-544.020
TAGCACG254.4436245E-544.01
ACGATTT207.8574085E-444.015
CTACGAT254.4436245E-544.013
TTGACGA502.7284841E-1144.018
GATATCG302.5283407E-644.01
TATTACG351.4465695E-744.01
CGGTCGT351.4465695E-744.028
TAGTGCG207.8574085E-444.01
ATCGCAC551.8189894E-1244.020
TACCGGC207.8574085E-444.037
TATAGCG1300.042.3076931
CTTTACG1200.042.16666436
CGTTTTT104900.041.7979051
CACGACG900.041.55555326
GCGTTAT750.041.06666614
Produced by FastQC (version 0.11.5)