FastQCFastQC Report
Thu 26 May 2016
SRR1546189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546189_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1346804
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179351.3316711266078805No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42830.3180121235161167No Hit
AGGCATGGAGTACCAGAGTGCTTTATATTTAGATTATATTTTAAGTCATG21250.15778093917154984No Hit
GAGCGAGGGAGTTTTCTTTTTCTTTATTGGTTGATATTACTCTCACTGGA19820.14716321008847613No Hit
AGAGATGGGATCCTGCAACTTCTCAAACTGTACTAAATTGATATTTCTTG18460.13706522998149692No Hit
AGACGTGGGTGCCCTTCTTTTATTTTATGTGTATCTATAAGTGCCTACAT18250.13550598305321337No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG18240.1354317331994856No Hit
AGTTTTAGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT16580.12310625748067276No Hit
AAGGTGGGGTTTGTAAATTAACTTTTATAAACTTTACCTTTTTATACATA16300.1210272615762947No Hit
TTTATAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA15690.11649802049889962No Hit
TTTATAGGGAGTAATCCATGGTTTTCTTGCATTTCCTTCCACCCATCAAG15390.11427052488706597No Hit
TCTTGGTGGGGTATGTGAATGTGTGTATTTTATAGTTGTTACGTATTACC15240.11315677708114914No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC14920.11078078176185993No Hit
TGTGAGGGTTTATAAAACTGAAGTACATGTGTAAATTAGAAACTAACATT14660.10885028556493744No Hit
GTAGTAGGGTGTATTCTATGGGGATTCTCTGGCTTTCTCTGAGTTCTTTG14600.1084047864425707No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG14100.10469229375618129No Hit
TTTTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT14090.10461804390245351No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13740.10201929902198092No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC13520.10038580223996958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG302.5280424E-644.0000042
GTCGAAA302.5280424E-644.00000422
ACTAGGT302.5280424E-644.00000417
CATCGAG600.044.0000041
CGATGCG302.5280424E-644.00000410
ATTTGCG600.044.0000041
CTAGGTC302.5280424E-644.00000418
GCGTAAG600.044.0000041
TGCGTAG600.044.0000041
AGTCGAA302.5280424E-644.00000421
AACCGTC207.8569644E-444.014
CGGAACC254.4432498E-544.039
ACACGAT408.314601E-944.029
CACGGTC254.4432498E-544.018
CGACGGT750.044.028
GCGAATG1450.044.01
ACGTTGC254.4432498E-544.031
CGCATGT254.4432498E-544.038
GAATCGT551.8189894E-1244.09
CCGTGAT254.4432498E-544.017
Produced by FastQC (version 0.11.5)