##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546189_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1346804
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.837252488112597	31.0	31.0	34.0	30.0	34.0
2	32.19259966557866	33.0	31.0	34.0	30.0	34.0
3	32.11593372161057	33.0	31.0	34.0	30.0	34.0
4	35.872484043706436	37.0	35.0	37.0	35.0	37.0
5	35.847518272889005	37.0	35.0	37.0	35.0	37.0
6	35.88151356841827	37.0	35.0	37.0	35.0	37.0
7	36.1283631471246	37.0	35.0	37.0	35.0	37.0
8	36.03132155829653	37.0	35.0	37.0	35.0	37.0
9	37.7737480732163	39.0	38.0	39.0	35.0	39.0
10	37.446378240634864	39.0	37.0	39.0	35.0	39.0
11	37.26887876780883	39.0	37.0	39.0	34.0	39.0
12	36.54388463354727	39.0	35.0	39.0	33.0	39.0
13	36.30820000534599	39.0	35.0	39.0	33.0	39.0
14	37.36773650805908	40.0	35.0	41.0	33.0	41.0
15	37.60679579211229	40.0	35.0	41.0	33.0	41.0
16	37.73523838658038	40.0	35.0	41.0	33.0	41.0
17	37.68366295318398	39.0	35.0	41.0	33.0	41.0
18	37.52760089812623	39.0	36.0	41.0	33.0	41.0
19	37.29182939759609	38.0	35.0	41.0	33.0	41.0
20	36.99307768613696	38.0	35.0	41.0	33.0	41.0
21	36.912581934713586	38.0	35.0	41.0	33.0	41.0
22	36.817880701275016	38.0	35.0	41.0	33.0	41.0
23	36.77518035289471	38.0	35.0	41.0	32.0	41.0
24	36.70946626235146	38.0	35.0	41.0	33.0	41.0
25	36.73262107923647	38.0	35.0	41.0	33.0	41.0
26	36.6599490349004	38.0	35.0	41.0	33.0	41.0
27	36.58167261160496	38.0	35.0	41.0	32.0	41.0
28	36.52887205562205	38.0	35.0	41.0	32.0	41.0
29	36.486416731759036	38.0	35.0	41.0	32.0	41.0
30	36.29492041900677	37.0	35.0	41.0	31.0	41.0
31	35.986875595855075	37.0	35.0	40.0	31.0	41.0
32	35.64449838283819	37.0	35.0	41.0	30.0	41.0
33	35.2476915720476	37.0	35.0	40.0	27.0	41.0
34	34.859208169859905	37.0	35.0	40.0	24.0	41.0
35	34.60080531391353	37.0	34.0	40.0	23.0	41.0
36	34.45376016109248	37.0	34.0	40.0	22.0	41.0
37	34.406584031529455	37.0	34.0	40.0	22.0	41.0
38	34.40117641468246	37.0	34.0	40.0	22.0	41.0
39	34.35152330999908	37.0	34.0	40.0	21.0	41.0
40	34.20080056192289	37.0	34.0	40.0	21.0	41.0
41	34.10844191136943	37.0	34.0	40.0	19.0	41.0
42	34.020884256358016	36.0	34.0	40.0	18.0	41.0
43	33.85479921354555	36.0	33.0	40.0	18.0	41.0
44	33.71012560105257	36.0	33.0	40.0	18.0	41.0
45	33.65547028372354	36.0	33.0	40.0	18.0	41.0
46	33.582488617497425	36.0	33.0	40.0	18.0	41.0
47	33.53219250908076	36.0	33.0	40.0	18.0	41.0
48	33.46918111321321	36.0	33.0	40.0	18.0	41.0
49	33.43180076685249	36.0	33.0	40.0	18.0	41.0
50	33.20522214071238	35.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	1.0
13	11.0
14	11.0
15	19.0
16	59.0
17	195.0
18	436.0
19	985.0
20	1818.0
21	2912.0
22	4653.0
23	7491.0
24	11335.0
25	18516.0
26	29304.0
27	36856.0
28	36580.0
29	32881.0
30	32702.0
31	34675.0
32	42007.0
33	54506.0
34	98053.0
35	206038.0
36	78497.0
37	90709.0
38	152951.0
39	372599.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.907481712261028	24.66877140252034	30.854303967021185	18.569442918197453
2	33.51979946599506	24.025990418798877	30.53139135315903	11.922818762047038
3	24.063857844200047	24.13194496006843	40.50723045075601	11.296966744975512
4	21.588516220623045	26.678714942931563	38.995354929150785	12.737413907294604
5	20.08310043629214	29.093320186159232	38.41702281846505	12.406556559083578
6	18.56788367126917	37.85806991960226	35.292143474477356	8.281902934651217
7	77.05441920279416	5.039486072212438	15.522080421501569	2.3840143034918224
8	78.69118297836954	3.7364753891434836	14.754114184395057	2.8182274480919274
9	73.8622694913291	5.427887057062498	17.4556208624269	3.254222589181499
10	43.594910618026084	25.731880808194806	21.574037499146126	9.099171074632984
11	39.79168461038132	18.158469977814143	29.690140510423195	12.359704901381344
12	33.574744357753616	20.294638269562608	33.79845916703544	12.332158205648334
13	17.189583636520236	38.16650381198749	32.020694919231005	12.623217632261262
14	10.457646398436594	39.06099179984616	37.13108960175349	13.350272199963767
15	10.029001992866075	22.230034956831133	55.729341463197315	12.011621587105473
16	10.896834283236462	19.823819947074707	50.32231861503232	18.957027154656505
17	11.142378549514257	22.230406206099772	36.965289678379335	29.661925566006637
18	15.893032690725601	23.693202574390927	43.5014300521828	16.912334682700674
19	21.38930386307139	23.034532121971722	36.857998639742675	18.71816537521421
20	22.468376987297333	22.24377117977078	38.17392879736027	17.11392303557162
21	17.429336414207263	26.599416099150286	38.238451920249716	17.732795566392735
22	17.709852361590848	25.11182027971405	33.07749308733862	24.100834271356486
23	13.143931856454245	28.368864363337202	34.70156013792653	23.785643642282025
24	12.248478620497117	23.991686986376635	47.99555094876463	15.764283444361615
25	11.58876867012572	27.244350328629853	43.99652807683969	17.17035292440474
26	12.112081639199172	32.072224317718096	37.62403438065227	18.191659662430464
27	12.500259874488048	34.763781515350416	35.473090368011974	17.262868242149562
28	10.73578635050089	30.14239636947915	43.286105476372214	15.835711803647747
29	12.806020772139078	25.161864681126577	40.33578753849854	21.696327008235794
30	15.037451626220296	30.393583624640257	37.876409633473024	16.692555115666423
31	20.967787443458736	29.655540078586046	33.098802795358495	16.277869682596723
32	22.835097014858878	26.533779228454918	34.242621792035074	16.38850196465113
33	21.545896804583293	27.904580027977342	32.213521789362076	18.336001378077285
34	16.33370557260002	26.677378445564464	35.06248867689731	21.92642730493821
35	17.159066946638116	26.245838295698558	34.61201481433081	21.983079943332513
36	23.429392844096096	26.001853276349046	33.93893989028842	16.629813989266438
37	16.46208356969537	31.090492751729283	35.848052129337304	16.599371549238047
38	15.416942628623021	30.426550559695396	33.82927285633247	20.327233955349108
39	17.224555317626024	30.28963382942136	32.655976667725966	19.829834185226655
40	18.66648747701967	24.918696410168074	32.94139310545558	23.473423007356676
41	14.08497450260023	26.175152434949705	32.8292015764729	26.910671485977172
42	17.087564337498254	25.787493948636918	32.115066483319026	25.0098752305458
43	17.661738456375243	24.991758266236218	35.16547322401775	22.181030053370794
44	15.217284771949	29.062877746130837	33.979628810131246	21.740208671788917
45	15.454067555486917	34.51058951413866	30.240034927131195	19.795308003243235
46	19.729893882109053	29.705732979706028	33.09620405047802	17.468169087706897
47	17.230346806216794	28.537411531299284	33.110534272247484	21.12170739023644
48	17.449755123982406	27.225193866368087	34.91859246037286	20.406458549276657
49	19.09037989195161	24.27153468507667	36.20140718322785	20.43667823974387
50	17.803629926849045	30.15345959768459	32.27559466707851	19.76731580838786
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5395.0
1	5843.5
2	6292.0
3	18137.5
4	29983.0
5	21700.5
6	13418.0
7	14956.0
8	16494.0
9	17842.0
10	19190.0
11	19223.0
12	19256.0
13	17864.5
14	16473.0
15	14999.5
16	13526.0
17	12805.5
18	12085.0
19	12323.0
20	12561.0
21	11842.0
22	11123.0
23	11956.0
24	12789.0
25	17299.5
26	21810.0
27	23483.5
28	25157.0
29	29729.0
30	34301.0
31	44146.0
32	53991.0
33	57720.0
34	61449.0
35	68481.5
36	75514.0
37	72238.0
38	68962.0
39	78405.0
40	87848.0
41	103438.0
42	119028.0
43	120920.0
44	122812.0
45	109554.0
46	96296.0
47	92319.0
48	88342.0
49	88238.0
50	88134.0
51	82557.0
52	76980.0
53	61547.0
54	46114.0
55	36138.5
56	26163.0
57	21503.0
58	16843.0
59	15118.5
60	13394.0
61	12499.5
62	11605.0
63	9635.0
64	7665.0
65	6435.5
66	5206.0
67	4635.0
68	4064.0
69	3234.5
70	2405.0
71	2117.5
72	1830.0
73	1501.5
74	1173.0
75	821.5
76	470.0
77	438.5
78	407.0
79	264.5
80	122.0
81	101.5
82	81.0
83	57.0
84	33.0
85	21.5
86	10.0
87	7.0
88	4.0
89	2.5
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1346804.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.9165547959646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.17021392983744	17.45558881343466
2	9.58882422348058	4.394856314925309
3	3.897116143100874	2.6792542691882817
4	2.063626948954736	1.8916488021660178
5	1.2794036604869778	1.465976204585376
6	0.8868157619693059	1.2193657201856818
7	0.5934093013116459	0.9519227738960382
8	0.45084508006308965	0.8265452785405473
9	0.3621772302007927	0.7469868907572436
>10	3.364037350004647	17.156487217054522
>50	0.7649891480285365	12.194611772746535
>100	0.5024002517631089	23.362419594554613
>500	0.06182386517809407	9.815297375109822
>1k	0.013991716856094977	4.5016637863645546
>5k	0.0	0.0
>10k+	3.25388764095232E-4	1.3373751864907781
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17935	1.3316711266078805	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4283	0.3180121235161167	No Hit
AGGCATGGAGTACCAGAGTGCTTTATATTTAGATTATATTTTAAGTCATG	2125	0.15778093917154984	No Hit
GAGCGAGGGAGTTTTCTTTTTCTTTATTGGTTGATATTACTCTCACTGGA	1982	0.14716321008847613	No Hit
AGAGATGGGATCCTGCAACTTCTCAAACTGTACTAAATTGATATTTCTTG	1846	0.13706522998149692	No Hit
AGACGTGGGTGCCCTTCTTTTATTTTATGTGTATCTATAAGTGCCTACAT	1825	0.13550598305321337	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1824	0.1354317331994856	No Hit
AGTTTTAGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1658	0.12310625748067276	No Hit
AAGGTGGGGTTTGTAAATTAACTTTTATAAACTTTACCTTTTTATACATA	1630	0.1210272615762947	No Hit
TTTATAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1569	0.11649802049889962	No Hit
TTTATAGGGAGTAATCCATGGTTTTCTTGCATTTCCTTCCACCCATCAAG	1539	0.11427052488706597	No Hit
TCTTGGTGGGGTATGTGAATGTGTGTATTTTATAGTTGTTACGTATTACC	1524	0.11315677708114914	No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1492	0.11078078176185993	No Hit
TGTGAGGGTTTATAAAACTGAAGTACATGTGTAAATTAGAAACTAACATT	1466	0.10885028556493744	No Hit
GTAGTAGGGTGTATTCTATGGGGATTCTCTGGCTTTCTCTGAGTTCTTTG	1460	0.1084047864425707	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1410	0.10469229375618129	No Hit
TTTTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1409	0.10461804390245351	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1374	0.10201929902198092	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC	1352	0.10038580223996958	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12748699885061227	0.0
2	0.0	0.0	0.0	0.539425187332381	0.0
3	0.0	0.0	0.0	0.7455427812807208	0.0
4	0.0	0.0	0.0	1.074989382270917	0.0
5	0.0	0.0	0.0	1.8048654444150745	0.0
6	0.0	0.0	0.0	2.4802421139230355	0.0
7	0.0	0.0	0.0	2.953659181291413	0.0
8	0.0	0.0	0.0	4.0253815699983075	0.0
9	0.0	0.0	0.0	4.529538076809989	0.0
10	0.0	0.0	0.0	5.495528673808512	0.0
11	0.0	0.0	0.0	6.547723350984999	0.0
12	0.0	0.0	0.0	7.503021969046721	0.0
13	0.0	0.0	0.0	7.829721325448989	0.0
14	0.0	0.0	0.0	7.950674337171556	0.0
15	0.0	0.0	0.0	8.136150471783571	0.0
16	0.0	0.0	0.0	8.549796406901079	0.0
17	0.0	0.0	0.0	9.079717612956303	0.0
18	0.0	0.0	0.0	9.864167317590384	0.0
19	0.0	0.0	0.0	10.191386422968748	0.0
20	0.0	0.0	0.0	10.606146105892172	0.0
21	0.0	0.0	0.0	11.084834912875221	0.0
22	0.0	0.0	0.0	11.520236055134971	0.0
23	0.0	0.0	0.0	12.039539532107122	0.0
24	0.0	0.0	0.0	12.38606359945471	0.0
25	0.0	0.0	0.0	12.673781782649888	0.0
26	0.0	0.0	0.0	12.985334168891688	0.0
27	0.0	0.0	0.0	13.284858078829584	0.0
28	0.0	0.0	0.0	13.649796109901663	0.0
29	0.0	0.0	0.0	13.950508017499205	0.0
30	0.0	0.0	0.0	14.421400589840838	0.0
31	0.0	0.0	0.0	14.854351486927571	0.0
32	0.0	0.0	0.0	15.205776044621192	0.0
33	0.0	0.0	0.0	15.535296895465116	0.0
34	0.0	0.0	0.0	15.85575926415425	0.0
35	7.424985372778816E-5	0.0	0.0	16.32138009688121	0.0
36	7.424985372778816E-5	0.0	0.0	16.67243340530619	0.0
37	7.424985372778816E-5	0.0	0.0	17.008413993424433	0.0
38	7.424985372778816E-5	0.0	0.0	17.295463927936062	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	30	2.5280424E-6	44.000004	2
GTCGAAA	30	2.5280424E-6	44.000004	22
ACTAGGT	30	2.5280424E-6	44.000004	17
CATCGAG	60	0.0	44.000004	1
CGATGCG	30	2.5280424E-6	44.000004	10
ATTTGCG	60	0.0	44.000004	1
CTAGGTC	30	2.5280424E-6	44.000004	18
GCGTAAG	60	0.0	44.000004	1
TGCGTAG	60	0.0	44.000004	1
AGTCGAA	30	2.5280424E-6	44.000004	21
AACCGTC	20	7.8569644E-4	44.0	14
CGGAACC	25	4.4432498E-5	44.0	39
ACACGAT	40	8.314601E-9	44.0	29
CACGGTC	25	4.4432498E-5	44.0	18
CGACGGT	75	0.0	44.0	28
GCGAATG	145	0.0	44.0	1
ACGTTGC	25	4.4432498E-5	44.0	31
CGCATGT	25	4.4432498E-5	44.0	38
GAATCGT	55	1.8189894E-12	44.0	9
CCGTGAT	25	4.4432498E-5	44.0	17
>>END_MODULE