FastQCFastQC Report
Thu 26 May 2016
SRR1546188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546188_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1380387
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184371.3356399328594082No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43310.3137525925700546No Hit
AGGCATGGAGTACCAGAGTGCTTTATATTTAGATTATATTTTAAGTCATG22320.1616937858730921No Hit
GAGCGAGGGAGTTTTCTTTTTCTTTATTGGTTGATATTACTCTCACTGGA20070.14539400907136912No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG19670.14249627097328502No Hit
AGAGATGGGATCCTGCAACTTCTCAAACTGTACTAAATTGATATTTCTTG18770.13597636025259582No Hit
AGACGTGGGTGCCCTTCTTTTATTTTATGTGTATCTATAAGTGCCTACAT17720.12836979774512508No Hit
AAGGTGGGGTTTGTAAATTAACTTTTATAAACTTTACCTTTTTATACATA17000.12315386916857375No Hit
AGTTTTAGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT16990.12308142571612164No Hit
TTTATAGGGAGTAATCCATGGTTTTCTTGCATTTCCTTCCACCCATCAAG16460.11924192273616023No Hit
TCTTGGTGGGGTATGTGAATGTGTGTATTTTATAGTTGTTACGTATTACC16340.11837260130673499No Hit
TTTATAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA16010.1159819673758156No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC15340.11112825606152477No Hit
GTAGTAGGGTGTATTCTATGGGGATTCTCTGGCTTTCTCTGAGTTCTTTG15260.11054870844190796No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG14970.108447848320797No Hit
TGTGAGGGTTTATAAAACTGAAGTACATGTGTAAATTAGAAACTAACATT14810.10728875308156337No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14270.10337680664914983No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA14220.10301458938688933No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC13930.10091372926577837No Hit
TTTTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT13830.10018929474125735No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAA302.5280897E-644.010
AAGTACG408.314601E-944.01
GGTACGA207.857032E-444.08
ACACGAT207.857032E-444.029
CGTATTA1650.044.042
GATCGTA207.857032E-444.01
CGTATAA207.857032E-444.036
GATCGGC254.4433073E-544.09
GTCGAAA207.857032E-444.022
CGAGTGA207.857032E-444.033
GACACGT502.7284841E-1144.014
GTTTACG302.5280897E-644.01
GTTTACC207.857032E-444.021
CGCATGT207.857032E-444.038
GCTATCC351.4463694E-744.023
CCGTGAT351.4463694E-744.017
TTCGTAC2500.044.033
CGACATA351.4463694E-744.020
TCGATTG351.4463694E-744.031
CGCGACC254.4433073E-544.010
Produced by FastQC (version 0.11.5)