##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546188_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1380387
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.773706214271794	31.0	31.0	34.0	30.0	34.0
2	32.16646853382421	33.0	31.0	34.0	30.0	34.0
3	32.2051671016896	33.0	31.0	34.0	30.0	34.0
4	35.90692610115859	37.0	35.0	37.0	35.0	37.0
5	35.808201613025915	37.0	35.0	37.0	35.0	37.0
6	35.871126720260335	37.0	35.0	37.0	35.0	37.0
7	36.10784149662377	37.0	35.0	37.0	35.0	37.0
8	36.011644560547154	37.0	35.0	37.0	35.0	37.0
9	37.74872916073536	39.0	38.0	39.0	35.0	39.0
10	37.44586047246171	39.0	37.0	39.0	35.0	39.0
11	37.26981201648523	39.0	37.0	39.0	34.0	39.0
12	36.53332145260713	39.0	35.0	39.0	33.0	39.0
13	36.24552462461614	39.0	35.0	39.0	32.0	39.0
14	37.29831344398346	40.0	35.0	41.0	33.0	41.0
15	37.5803300089033	40.0	35.0	41.0	33.0	41.0
16	37.69678213428553	40.0	35.0	41.0	33.0	41.0
17	37.652088146295206	39.0	35.0	41.0	33.0	41.0
18	37.51712671881146	39.0	36.0	41.0	33.0	41.0
19	37.28618423673941	38.0	35.0	41.0	33.0	41.0
20	36.99090544897916	38.0	35.0	41.0	33.0	41.0
21	36.89825606876912	38.0	35.0	41.0	33.0	41.0
22	36.8123077079109	38.0	35.0	41.0	33.0	41.0
23	36.75407258978822	38.0	35.0	41.0	32.0	41.0
24	36.694274866396164	38.0	35.0	41.0	32.0	41.0
25	36.709126498583366	38.0	35.0	41.0	33.0	41.0
26	36.65479173594072	38.0	35.0	41.0	33.0	41.0
27	36.561237537009546	38.0	35.0	41.0	32.0	41.0
28	36.509370198357416	38.0	35.0	41.0	32.0	41.0
29	36.477027819010175	38.0	35.0	41.0	32.0	41.0
30	36.28552500132209	37.0	35.0	41.0	31.0	41.0
31	35.984883949211344	37.0	35.0	40.0	31.0	41.0
32	35.6550010975183	37.0	35.0	40.0	30.0	41.0
33	35.23684227683976	37.0	35.0	40.0	27.0	41.0
34	34.92178642656009	37.0	35.0	40.0	24.0	41.0
35	34.634695922230506	37.0	34.0	40.0	23.0	41.0
36	34.48185762398516	37.0	34.0	40.0	22.0	41.0
37	34.36504617907877	37.0	34.0	40.0	22.0	41.0
38	34.37196742652604	37.0	34.0	40.0	22.0	41.0
39	34.32880416868603	37.0	34.0	40.0	21.0	41.0
40	34.19837697689126	37.0	34.0	40.0	21.0	41.0
41	34.12070890264832	37.0	34.0	40.0	19.0	41.0
42	34.016906128498746	36.0	34.0	40.0	18.0	41.0
43	33.87823124964231	36.0	34.0	40.0	18.0	41.0
44	33.727361964434614	36.0	33.0	40.0	18.0	41.0
45	33.66099869094681	36.0	33.0	40.0	18.0	41.0
46	33.6035894281821	36.0	33.0	40.0	18.0	41.0
47	33.530243330312445	36.0	33.0	40.0	18.0	41.0
48	33.48276099383724	36.0	33.0	40.0	18.0	41.0
49	33.44372339061437	36.0	33.0	40.0	18.0	41.0
50	33.18797047494652	35.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	1.0
11	2.0
12	5.0
13	3.0
14	11.0
15	25.0
16	64.0
17	206.0
18	474.0
19	972.0
20	1827.0
21	3065.0
22	5098.0
23	7670.0
24	11708.0
25	18662.0
26	29529.0
27	37425.0
28	37321.0
29	33824.0
30	33543.0
31	36318.0
32	43622.0
33	56191.0
34	103389.0
35	208188.0
36	80671.0
37	93879.0
38	157451.0
39	379241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.015675314241587	24.864693741682586	30.76941466414853	18.350216279927295
2	33.55877735736428	24.072089928404136	30.50376452400667	11.865368190224915
3	24.04927024088172	24.110484958203752	40.583474054739725	11.256770746174805
4	21.641104994468943	26.64100719580813	39.022172767492016	12.69571504223091
5	20.096683031642577	29.115603088119492	38.32316589478168	12.464547985456251
6	18.656289866537428	37.80975914725363	35.178395623835925	8.355555362373016
7	77.09222123940606	5.028589808510222	15.47942714615539	2.3997618059283377
8	78.69952411896084	3.763582241791614	14.659657038207403	2.877236601040143
9	73.8220513522657	5.482810255384903	17.410769588528435	3.2843688038209575
10	43.67311485836943	25.62491533171495	21.478759217523784	9.223210592391844
11	39.79499951825104	18.154401627949262	29.637051058869723	12.413547794929974
12	33.53074174126532	20.295540308623597	33.75292580993591	12.420792140175182
13	17.219881091317145	38.11641228148338	32.02688811181213	12.636818515387352
14	10.487711054943286	38.98732746686255	37.136035039449084	13.388926438745077
15	9.979447792539338	22.144949206273314	55.785515221456016	12.090087779731336
16	10.992714361986893	19.745766947964594	50.25105278447276	19.010465905575753
17	11.199612862190095	22.158785905691662	36.929426313055686	29.71217491906255
18	15.901772473951144	23.60359812139639	43.5326470040648	16.96198240058766
19	21.370890916822603	23.079976847072597	36.87364485466757	18.675487381437232
20	22.387634771987855	22.219928179561236	38.17813410297257	17.214302945478334
21	17.34484604679702	26.580009808843464	38.3204130435885	17.754731100771014
22	17.65468669293466	25.004219831105335	33.17316085996174	24.167932615998268
23	13.129071774799387	28.344007876052153	34.68947476323669	23.837445585911777
24	12.233598259038951	23.996966068211307	47.86505523451032	15.904380438239421
25	11.639272175121903	27.14296787784875	43.89066254608309	17.327097400946258
26	12.073932889834516	31.984726022485	37.657917670914024	18.28342341676646
27	12.48954097655223	34.72794223648875	35.48200613306268	17.300510653896335
28	10.667443260476952	30.120900877797315	43.27866025976773	15.932995601958003
29	12.902830872791471	25.069056721049964	40.261897569304836	21.766214836853724
30	15.038463851079445	30.356414541719097	37.85315277527244	16.751968831929016
31	20.893416121710796	29.65595879995972	33.04573282709849	16.404892251230997
32	22.74615741817331	26.553567948698444	34.29429573011047	16.405978903017775
33	21.516429812798872	27.840091220795326	32.19488447804855	18.44859448835725
34	16.33925848330939	26.58790614516074	35.08204583207463	21.990789539455243
35	17.202060002013926	26.16374973105368	34.474390152906395	22.159800114025995
36	23.44574383850326	25.807835049156502	34.01698219412383	16.729438918216413
37	16.391417769074902	30.906115458925647	35.92775069600047	16.774716075998978
38	15.415966681807348	30.38198708043469	33.82457238441104	20.377473853346924
39	17.33412441583411	30.02875280627824	32.66308651124648	19.974036266641164
40	18.721851191006582	24.82521206009619	32.862088675132405	23.59084807376482
41	14.073661951322347	25.909473212946803	32.87628759181302	27.14057724391783
42	17.160260129949066	25.664831674016053	32.10845943927319	25.066448756761687
43	17.798486946052087	24.813041560084237	35.09718651363712	22.291284980226557
44	15.33367091982176	28.891463046232683	33.965185125620565	21.809680908324985
45	15.583600830781513	34.296251703326675	30.28665149700772	19.833495968884087
46	19.786552611695125	29.56743290106325	33.051383416389754	17.59463107085187
47	17.206406609161053	28.44803667377337	33.10289071108319	21.24266600598238
48	17.497701731470958	27.153182404644493	34.87384334972729	20.47527251415726
49	19.168609962278694	24.11019518439394	36.156382231939304	20.56481262138806
50	17.918380859860314	29.864523499569323	32.23559769832663	19.981497942243735
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5502.0
1	6055.0
2	6608.0
3	18519.5
4	30431.0
5	22045.0
6	13659.0
7	15151.5
8	16644.0
9	18140.0
10	19636.0
11	19508.0
12	19380.0
13	18140.0
14	16900.0
15	15196.5
16	13493.0
17	12911.0
18	12329.0
19	12519.0
20	12709.0
21	12113.0
22	11517.0
23	12312.5
24	13108.0
25	17681.5
26	22255.0
27	23918.5
28	25582.0
29	30130.0
30	34678.0
31	44839.5
32	55001.0
33	58725.5
34	62450.0
35	69691.5
36	76933.0
37	73510.0
38	70087.0
39	79724.0
40	89361.0
41	105338.0
42	121315.0
43	123001.5
44	124688.0
45	111706.0
46	98724.0
47	95227.5
48	91731.0
49	91783.0
50	91835.0
51	86510.5
52	81186.0
53	64890.5
54	48595.0
55	37835.5
56	27076.0
57	22240.5
58	17405.0
59	15555.5
60	13706.0
61	12846.0
62	11986.0
63	9954.5
64	7923.0
65	6671.5
66	5420.0
67	4746.5
68	4073.0
69	3187.5
70	2302.0
71	2042.5
72	1783.0
73	1485.0
74	1187.0
75	835.5
76	484.0
77	460.5
78	437.0
79	279.0
80	121.0
81	110.0
82	99.0
83	66.5
84	34.0
85	19.5
86	5.0
87	5.0
88	5.0
89	4.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1380387.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.896635985227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.01782366423126	17.405524368290777
2	9.605383572966193	4.39861942337372
3	3.991792552497201	2.741958630092056
4	2.0200264334630487	1.8500723971015918
5	1.330913211009866	1.5236717660211254
6	0.8502739052404882	1.168104725761731
7	0.6708645705618639	1.0752379307278441
8	0.4541065936917146	0.8318010699400455
9	0.3549413403529668	0.7314266399553399
>10	3.3691989159711557	17.20259773312835
>50	0.7596257267113051	12.083200753730473
>100	0.495809154247975	22.82014155226309
>500	0.06523804661155097	10.291739275975836
>1k	0.013684078069739961	4.532492353110935
>5k	0.0	0.0
>10k+	3.1823437371488275E-4	1.3434113805270769
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18437	1.3356399328594082	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4331	0.3137525925700546	No Hit
AGGCATGGAGTACCAGAGTGCTTTATATTTAGATTATATTTTAAGTCATG	2232	0.1616937858730921	No Hit
GAGCGAGGGAGTTTTCTTTTTCTTTATTGGTTGATATTACTCTCACTGGA	2007	0.14539400907136912	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1967	0.14249627097328502	No Hit
AGAGATGGGATCCTGCAACTTCTCAAACTGTACTAAATTGATATTTCTTG	1877	0.13597636025259582	No Hit
AGACGTGGGTGCCCTTCTTTTATTTTATGTGTATCTATAAGTGCCTACAT	1772	0.12836979774512508	No Hit
AAGGTGGGGTTTGTAAATTAACTTTTATAAACTTTACCTTTTTATACATA	1700	0.12315386916857375	No Hit
AGTTTTAGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1699	0.12308142571612164	No Hit
TTTATAGGGAGTAATCCATGGTTTTCTTGCATTTCCTTCCACCCATCAAG	1646	0.11924192273616023	No Hit
TCTTGGTGGGGTATGTGAATGTGTGTATTTTATAGTTGTTACGTATTACC	1634	0.11837260130673499	No Hit
TTTATAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1601	0.1159819673758156	No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1534	0.11112825606152477	No Hit
GTAGTAGGGTGTATTCTATGGGGATTCTCTGGCTTTCTCTGAGTTCTTTG	1526	0.11054870844190796	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1497	0.108447848320797	No Hit
TGTGAGGGTTTATAAAACTGAAGTACATGTGTAAATTAGAAACTAACATT	1481	0.10728875308156337	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1427	0.10337680664914983	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1422	0.10301458938688933	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC	1393	0.10091372926577837	No Hit
TTTTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1383	0.10018929474125735	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13090531858094867	0.0
2	0.0	0.0	0.0	0.5515844469703062	0.0
3	0.0	0.0	0.0	0.7540638965739318	0.0
4	0.0	0.0	0.0	1.0885353165452876	0.0
5	0.0	0.0	0.0	1.8284727398910596	0.0
6	0.0	0.0	0.0	2.515888660209057	0.0
7	0.0	0.0	0.0	2.9916248124620126	0.0
8	0.0	0.0	0.0	4.086462709370633	0.0
9	0.0	0.0	0.0	4.592262894391211	0.0
10	0.0	0.0	0.0	5.612411591821714	0.0
11	0.0	0.0	0.0	6.710147226828418	0.0
12	0.0	0.0	0.0	7.705954924235015	0.0
13	0.0	0.0	0.0	8.052162183503611	0.0
14	0.0	0.0	0.0	8.178793338389886	0.0
15	0.0	0.0	0.0	8.375042651082632	0.0
16	0.0	0.0	0.0	8.818976127709114	0.0
17	0.0	0.0	0.0	9.381137318737427	0.0
18	0.0	0.0	0.0	10.207861998120817	0.0
19	0.0	0.0	0.0	10.553562153222249	0.0
20	0.0	0.0	0.0	10.989744180436356	0.0
21	0.0	0.0	0.0	11.486416490447969	0.0
22	0.0	0.0	0.0	11.930277523622	0.0
23	0.0	0.0	0.0	12.474038077727478	0.0
24	0.0	0.0	0.0	12.844441450115076	0.0
25	0.0	0.0	0.0	13.151167027797277	0.0
26	0.0	0.0	0.0	13.469773331681623	0.0
27	0.0	0.0	0.0	13.77468782305252	0.0
28	0.0	0.0	0.0	14.146395177584257	0.0
29	0.0	0.0	0.0	14.471738722546649	0.0
30	0.0	0.0	0.0	14.940085642649489	0.0
31	0.0	0.0	0.0	15.391263464521181	0.0
32	0.0	0.0	0.0	15.740440905340314	0.0
33	0.0	0.0	0.0	16.07042083125964	0.0
34	0.0	0.0	0.0	16.405471798850613	0.0
35	0.0	0.0	0.0	16.872804510619122	0.0
36	0.0	0.0	0.0	17.2390061627645	0.0
37	0.0	0.0	0.0	17.585792969652715	0.0
38	0.0	0.0	0.0	17.87824718720185	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGTAA	30	2.5280897E-6	44.0	10
AAGTACG	40	8.314601E-9	44.0	1
GGTACGA	20	7.857032E-4	44.0	8
ACACGAT	20	7.857032E-4	44.0	29
CGTATTA	165	0.0	44.0	42
GATCGTA	20	7.857032E-4	44.0	1
CGTATAA	20	7.857032E-4	44.0	36
GATCGGC	25	4.4433073E-5	44.0	9
GTCGAAA	20	7.857032E-4	44.0	22
CGAGTGA	20	7.857032E-4	44.0	33
GACACGT	50	2.7284841E-11	44.0	14
GTTTACG	30	2.5280897E-6	44.0	1
GTTTACC	20	7.857032E-4	44.0	21
CGCATGT	20	7.857032E-4	44.0	38
GCTATCC	35	1.4463694E-7	44.0	23
CCGTGAT	35	1.4463694E-7	44.0	17
TTCGTAC	250	0.0	44.0	33
CGACATA	35	1.4463694E-7	44.0	20
TCGATTG	35	1.4463694E-7	44.0	31
CGCGACC	25	4.4433073E-5	44.0	10
>>END_MODULE