FastQCFastQC Report
Thu 26 May 2016
SRR1546187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546187_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2236086
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376311.682895917241108No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29090.13009338639032667No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC24920.11144472976441873No Hit
AATGAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTAT23420.10473657989898422No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22780.10187443595639882No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGA207.858082E-444.040
GTGTCGA207.858082E-444.039
TATTACG850.044.01
TCGAGTA750.044.010
CCGTTAG408.3182385E-944.01
AATCGTC254.4441953E-544.016
TACCGTA302.5287954E-644.014
ATTACGT1050.041.90476242
CGTTTTT213450.041.89741
CGGATCG800.041.256
CGACGAA1350.040.7407444
TGCGTAG1350.040.740741
CCGCTTA650.040.61538321
TAAATCG650.040.61538333
TCGTACA1200.040.33333232
ATCTCGA1000.039.611
TTACGCG1000.039.61
TTTAGCG1900.039.368421
GCTACGA950.039.3684210
TAGTGCG950.039.368421
Produced by FastQC (version 0.11.5)