##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546187_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2236086
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9870139162805	33.0	31.0	34.0	30.0	34.0
2	32.365200175664086	33.0	31.0	34.0	31.0	34.0
3	32.29124416502764	34.0	31.0	34.0	30.0	34.0
4	36.01053000644877	37.0	35.0	37.0	35.0	37.0
5	36.002832180873185	37.0	35.0	37.0	35.0	37.0
6	36.05319249796296	37.0	35.0	37.0	35.0	37.0
7	36.22889101760845	37.0	35.0	37.0	35.0	37.0
8	36.13439912418395	37.0	36.0	37.0	35.0	37.0
9	37.86284561506132	39.0	38.0	39.0	35.0	39.0
10	37.55523043389208	39.0	37.0	39.0	35.0	39.0
11	37.47249926881167	39.0	37.0	39.0	35.0	39.0
12	37.185241980854045	39.0	37.0	39.0	34.0	39.0
13	37.08934540084773	39.0	37.0	39.0	33.0	39.0
14	38.32409978864856	40.0	37.0	41.0	33.0	41.0
15	38.41942215102639	40.0	37.0	41.0	34.0	41.0
16	38.473069908760216	40.0	37.0	41.0	34.0	41.0
17	38.392115956184156	40.0	37.0	41.0	34.0	41.0
18	38.1992669333827	40.0	37.0	41.0	34.0	41.0
19	38.02454780361757	40.0	36.0	41.0	34.0	41.0
20	37.75838138604687	40.0	35.0	41.0	34.0	41.0
21	37.705522059527226	39.0	35.0	41.0	34.0	41.0
22	37.625994259612554	39.0	35.0	41.0	33.0	41.0
23	37.53838179748006	39.0	35.0	41.0	33.0	41.0
24	37.49170783234634	39.0	35.0	41.0	33.0	41.0
25	37.48970433158653	39.0	35.0	41.0	33.0	41.0
26	37.42786771170697	39.0	35.0	41.0	33.0	41.0
27	37.345791709263416	39.0	35.0	41.0	33.0	41.0
28	37.284516785132595	39.0	35.0	41.0	33.0	41.0
29	37.219840829019994	39.0	35.0	41.0	33.0	41.0
30	37.04125691051239	39.0	35.0	41.0	33.0	41.0
31	36.81187172586385	39.0	35.0	41.0	32.0	41.0
32	36.49742093998174	39.0	35.0	41.0	31.0	41.0
33	36.144673326517854	39.0	35.0	41.0	30.0	41.0
34	35.72116591222341	39.0	35.0	41.0	26.0	41.0
35	35.48091128874292	39.0	35.0	41.0	24.0	41.0
36	35.34052133951914	39.0	35.0	41.0	23.0	41.0
37	35.306385800903904	39.0	35.0	41.0	23.0	41.0
38	35.233923471637496	39.0	35.0	41.0	23.0	41.0
39	35.132373262924595	39.0	35.0	41.0	23.0	41.0
40	35.01505532434799	39.0	35.0	41.0	22.0	41.0
41	34.875800394081445	39.0	35.0	41.0	21.0	41.0
42	34.81087534200384	38.0	35.0	41.0	20.0	41.0
43	34.67931868452287	38.0	35.0	41.0	19.0	41.0
44	34.564967984236745	38.0	34.0	41.0	18.0	41.0
45	34.48374212798613	38.0	34.0	40.0	20.0	41.0
46	34.409277192379896	38.0	34.0	40.0	19.0	41.0
47	34.35038589750126	38.0	34.0	40.0	19.0	41.0
48	34.24411896501297	38.0	34.0	40.0	18.0	41.0
49	34.18356986269759	38.0	34.0	40.0	18.0	41.0
50	34.05806932291513	38.0	34.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	3.0
11	7.0
12	7.0
13	20.0
14	27.0
15	28.0
16	112.0
17	232.0
18	604.0
19	1149.0
20	2311.0
21	3761.0
22	6392.0
23	9998.0
24	16068.0
25	26597.0
26	43504.0
27	54131.0
28	52930.0
29	46770.0
30	44519.0
31	47026.0
32	56157.0
33	72546.0
34	125315.0
35	223723.0
36	138321.0
37	174184.0
38	308004.0
39	781636.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.80272583433732	23.709732094382773	29.4516400532001	20.035902018079803
2	35.47922575428673	25.02189092906087	28.213762798031915	11.285120518620483
3	25.041255121672425	23.936825327827286	39.40850217746545	11.613417373034848
4	24.273574451072097	25.019565437107516	38.53170226905405	12.175157842766334
5	21.27301901626324	28.544608749395152	37.38948323096697	12.792889003374647
6	19.28503644314217	37.40996544855609	34.51150805469915	8.79349005360259
7	76.76028560618867	4.47509621722957	16.61098902278356	2.153629153798199
8	77.95080332330689	4.020373098351316	15.709860890860192	2.3189626874816085
9	71.77228425024798	6.213222568362755	18.27147971947412	3.7430134619151496
10	33.45492972989411	30.121113409770466	25.498527337499542	10.925429522835884
11	28.50386791921241	23.383850173919964	34.438120895171295	13.67416101169633
12	26.114961589133873	21.54662208877476	37.285014977062595	15.05340134502877
13	18.275057399402346	28.484145958608032	37.75328855866903	15.48750808332059
14	14.134832023455267	31.10121882610955	39.32098318222108	15.442965968214104
15	13.430073798592721	24.771900544075677	46.99094757536159	14.807078081970012
16	16.32244913657167	23.362562978346986	43.950724614348466	16.364263270732877
17	15.841653675216428	24.488503572760617	37.60687200760614	22.062970744416806
18	17.26051681375403	25.14648363256154	41.19349613565847	16.39950341802596
19	19.627330970275743	25.89913804746329	37.35974376656354	17.113787215697428
20	20.715616483444734	25.114687002199375	38.10358814464202	16.066108369713866
21	18.444639428000535	26.938141019620893	38.830438543061405	15.786781009317172
22	19.155256103745565	24.06888643817814	37.19409718588641	19.58176027218989
23	16.01521587273477	26.67008335099813	37.79948535074232	19.51521542552478
24	15.33890020330166	25.16763666513721	43.730250088771186	15.763213042789948
25	15.129471764502798	28.032329704671465	40.108564697422196	16.729633833403547
26	15.33116347045686	29.607000804083565	37.01145662555018	18.050379099909396
27	15.22557719157492	29.688169417455324	37.55097075872752	17.53528263224223
28	13.931262035538882	28.073159976852413	41.096496288604285	16.89908169900442
29	15.204916089989384	25.47334941500461	40.63569111384804	18.68604338115797
30	16.12634755550547	28.30271286524758	38.852485995619126	16.71845358362782
31	18.888808391090503	28.666205145955924	35.86387106757075	16.581115395382824
32	19.129899297254223	27.22261129491442	36.66464527750722	16.982844130324146
33	18.93214303922121	29.17114994682673	34.71226061967205	17.184446394280005
34	17.159581518778793	27.414643265062256	36.388448387047724	19.037326829111223
35	18.0597257887219	27.89821142836188	36.13622195210739	17.905840830808835
36	19.569104229443766	29.36027505203288	34.263530114673586	16.807090603849762
37	17.171164257546444	30.873097009685672	36.02195979939949	15.933778933368394
38	16.964463799692854	28.54000248648755	35.868164283484624	18.62736943033497
39	17.148490711001276	30.466806732835856	34.27207182550224	18.112630730660626
40	18.587523020134288	28.104241071228923	34.45913976474966	18.84909614388713
41	15.604587658971973	28.44273431343875	35.280888123265385	20.671789904323894
42	17.459525259761925	28.822415595822342	33.29979258400616	20.418266560409574
43	17.104127479891204	28.297882997344466	34.20852328577702	20.389466236987307
44	16.89845560501698	29.577395502677444	33.596248087059266	19.92790080524631
45	16.164449846741135	32.2536789729912	32.350365773051664	19.231505407216
46	18.676428366350848	30.036769605462403	33.24813983004232	18.038662198144436
47	16.96128860875655	29.96973282780716	34.46338825966443	18.60559030377186
48	16.67471644650519	27.95469405022884	36.247666681871806	19.122922821394166
49	18.317005696560866	27.808545825160568	35.59956996287263	18.274878515405938
50	17.004399651891745	29.735931444497215	34.72200979747648	18.53765910613456
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5411.0
1	9407.0
2	13403.0
3	39746.0
4	66089.0
5	46358.5
6	26628.0
7	27016.5
8	27405.0
9	28209.5
10	29014.0
11	27978.5
12	26943.0
13	26261.0
14	25579.0
15	23353.0
16	21127.0
17	20108.5
18	19090.0
19	20639.5
20	22189.0
21	23578.5
22	24968.0
23	29000.0
24	33032.0
25	38086.0
26	43140.0
27	51595.5
28	60051.0
29	77209.5
30	94368.0
31	99367.0
32	104366.0
33	117444.5
34	130523.0
35	133466.5
36	136410.0
37	140933.0
38	145456.0
39	149350.0
40	153244.0
41	153897.5
42	154551.0
43	148887.0
44	143223.0
45	138181.0
46	133139.0
47	126511.5
48	119884.0
49	111306.0
50	102728.0
51	89649.0
52	76570.0
53	66833.0
54	57096.0
55	52815.0
56	48534.0
57	45966.0
58	43398.0
59	41088.5
60	38779.0
61	35560.0
62	32341.0
63	28975.5
64	25610.0
65	21742.0
66	17874.0
67	15149.5
68	12425.0
69	10449.5
70	8474.0
71	6953.5
72	5433.0
73	4406.5
74	3380.0
75	2791.0
76	2202.0
77	1589.5
78	977.0
79	774.5
80	572.0
81	397.5
82	223.0
83	177.5
84	132.0
85	103.0
86	74.0
87	45.0
88	16.0
89	14.0
90	12.0
91	6.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2236086.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.332053779952133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.61727728194823	16.557359332319766
2	8.183798746282141	3.4915446995999098
3	3.1029636979201043	1.9857776544381245
4	1.6730770207382042	1.4276067593755786
5	1.132649780023864	1.2080873010660012
6	0.7935459211120344	1.0156778559614144
7	0.6082425763652506	0.9082544345196117
8	0.49613369817860864	0.8466840585274096
9	0.421826615993855	0.8098585252376519
>10	4.61278099805626	22.288969866756226
>50	0.7065173197735745	10.309155529140206
>100	0.5818842326825598	26.80737940517736
>500	0.06401993783647325	9.034064426605301
>1k	0.005070886165265208	1.613481734129011
>5k	0.0	0.0
>10k+	2.11286923552717E-4	1.6960984171464553
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37631	1.682895917241108	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2909	0.13009338639032667	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC	2492	0.11144472976441873	No Hit
AATGAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTAT	2342	0.10473657989898422	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2278	0.10187443595639882	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03595568327872899	0.0
2	0.0	0.0	0.0	0.1862182402644621	0.0
3	0.0	0.0	0.0	0.30294004792302265	0.0
4	0.0	0.0	0.0	0.5161250506465315	0.0
5	0.0	0.0	0.0	1.0146300276465217	0.0
6	0.0	0.0	0.0	1.4129152456569203	0.0
7	0.0	0.0	0.0	1.7078949557396272	0.0
8	0.0	0.0	0.0	2.2799659762638824	0.0
9	0.0	0.0	0.0	2.5098319116527716	0.0
10	0.0	0.0	0.0	3.0002871088142404	0.0
11	0.0	0.0	0.0	3.5149363664903763	0.0
12	0.0	0.0	0.0	3.9811080611389724	0.0
13	0.0	0.0	0.0	4.253011735684584	0.0
14	0.0	0.0	0.0	4.313072037479775	0.0
15	0.0	0.0	0.0	4.452064902691578	0.0
16	0.0	0.0	0.0	4.651386395693189	0.0
17	0.0	0.0	0.0	4.8680596363467235	0.0
18	0.0	0.0	0.0	5.171715220255392	0.0
19	0.0	0.0	0.0	5.360348394471411	0.0
20	0.0	0.0	0.0	5.558686025492758	0.0
21	0.0	0.0	0.0	5.80402542657125	0.0
22	0.0	0.0	0.0	6.078120430072905	0.0
23	0.0	0.0	0.0	6.3494427316301785	0.0
24	0.0	0.0	0.0	6.558379239438913	0.0
25	0.0	0.0	0.0	6.7532286325302335	0.0
26	0.0	0.0	0.0	6.940430734774959	0.0
27	0.0	0.0	0.0	7.141541067740686	0.0
28	0.0	0.0	0.0	7.336211576835596	0.0
29	0.0	0.0	0.0	7.544611432655095	0.0
30	0.0	0.0	0.0	7.810030562330787	0.0
31	0.0	0.0	0.0	8.04673881058242	0.0
32	0.0	0.0	0.0	8.269941317104978	0.0
33	0.0	0.0	0.0	8.491131378667905	0.0
34	0.0	0.0	0.0	8.713931396198536	0.0
35	4.472099910289676E-5	0.0	0.0	8.98677421172531	0.0
36	4.472099910289676E-5	0.0	0.0	9.214985470147392	0.0
37	4.472099910289676E-5	0.0	0.0	9.440245142628683	0.0
38	4.472099910289676E-5	0.0	0.0	9.693142392555563	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACCGA	20	7.858082E-4	44.0	40
GTGTCGA	20	7.858082E-4	44.0	39
TATTACG	85	0.0	44.0	1
TCGAGTA	75	0.0	44.0	10
CCGTTAG	40	8.3182385E-9	44.0	1
AATCGTC	25	4.4441953E-5	44.0	16
TACCGTA	30	2.5287954E-6	44.0	14
ATTACGT	105	0.0	41.904762	42
CGTTTTT	21345	0.0	41.8974	1
CGGATCG	80	0.0	41.25	6
CGACGAA	135	0.0	40.74074	44
TGCGTAG	135	0.0	40.74074	1
CCGCTTA	65	0.0	40.615383	21
TAAATCG	65	0.0	40.615383	33
TCGTACA	120	0.0	40.333332	32
ATCTCGA	100	0.0	39.6	11
TTACGCG	100	0.0	39.6	1
TTTAGCG	190	0.0	39.36842	1
GCTACGA	95	0.0	39.36842	10
TAGTGCG	95	0.0	39.36842	1
>>END_MODULE