FastQCFastQC Report
Thu 26 May 2016
SRR1546186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546186_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2261867
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381691.6874997513116379No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29930.13232431438276435No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC23370.10332172492900776No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23330.1031448798713629No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTACG302.5288082E-644.00000436
CGCATTA351.4469151E-744.019
CGGTCGA700.044.06
GCGTAAT207.858102E-444.013
AGTCGTA454.802132E-1044.022
TCGGTCG650.043.9999965
CGACGAA1450.042.4827644
CGTTTTT211400.041.8978231
TCGAGTA603.6379788E-1240.33333610
CGAATAT1150.040.1739114
CGGTCTA1100.040.031
ACGCGCC557.8216544E-1140.032
CACGACG1050.039.8095226
TCGATTG1550.039.7419321
CGACCCG1000.039.632
ATACGAC501.3496901E-939.62
TACCGTA452.3526809E-839.1111114
ATTGCGG4550.038.681322
AAGTGCG1650.038.6666681
ATGGGCG3250.038.5846185
Produced by FastQC (version 0.11.5)