FastQCFastQC Report
Thu 26 May 2016
SRR1546185_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546185_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3339146
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT624191.8693102967046065No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAA62810.188101987753755No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34740.10403857752850579No Hit
TTTCTAGGGATTATCAAACATTGATGGGGTTAATATTACAGTCTATGAAA34180.1023615020127901No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC207.8586384E-444.0000049
CGTTTTT342750.042.202771
TACGTTG800.041.2500041
TACGGGA12900.041.1007734
TGCTACG2650.040.679251
TATACGG4300.039.906982
CGACGGT2900.039.44827328
CTAAGCG900.039.111111
GCCCTAC29150.038.86792426
GCCCTAG29450.038.845520
CGAATAT4250.038.82353214
TAACGTT850.038.8235312
CGGTCTA2900.038.68965531
GCGTAAG1650.038.6666681
CAATTAT58750.038.64510744
ATTAGCG1200.038.51
ATACTCG3550.038.42253544
CCCTAGC29950.038.19699521
ACGGGAC8000.037.955
AGTACGG4700.037.914892
Produced by FastQC (version 0.11.5)