##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546185_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3339146
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.920911813978783	31.0	31.0	34.0	30.0	34.0
2	32.28523281102414	33.0	31.0	34.0	30.0	34.0
3	32.20119845014264	34.0	31.0	34.0	30.0	34.0
4	35.94488471004263	37.0	35.0	37.0	35.0	37.0
5	35.94311629380686	37.0	35.0	37.0	35.0	37.0
6	35.990103757068425	37.0	35.0	37.0	35.0	37.0
7	36.20104631543514	37.0	35.0	37.0	35.0	37.0
8	36.08953307222865	37.0	35.0	37.0	35.0	37.0
9	37.79271017200206	39.0	38.0	39.0	35.0	39.0
10	37.52563619560211	39.0	37.0	39.0	35.0	39.0
11	37.40699178772057	39.0	37.0	39.0	35.0	39.0
12	37.18111966353074	39.0	37.0	39.0	34.0	39.0
13	37.120773095875414	39.0	37.0	39.0	33.0	39.0
14	38.36889012939236	40.0	38.0	41.0	33.0	41.0
15	38.43081195012138	40.0	38.0	41.0	34.0	41.0
16	38.48726919996909	40.0	38.0	41.0	34.0	41.0
17	38.381012390593284	40.0	37.0	41.0	34.0	41.0
18	38.182955761742676	40.0	37.0	41.0	34.0	41.0
19	37.98933439867559	40.0	37.0	41.0	34.0	41.0
20	37.75584146365568	40.0	35.0	41.0	34.0	41.0
21	37.65355962272989	39.0	35.0	41.0	33.0	41.0
22	37.559236104081705	39.0	35.0	41.0	33.0	41.0
23	37.51133673100847	39.0	35.0	41.0	33.0	41.0
24	37.446344963652386	39.0	35.0	41.0	33.0	41.0
25	37.438663658312635	39.0	35.0	41.0	33.0	41.0
26	37.37737673045743	39.0	35.0	41.0	33.0	41.0
27	37.28681704843095	39.0	35.0	41.0	33.0	41.0
28	37.2124096999652	39.0	35.0	41.0	33.0	41.0
29	37.11725513050343	39.0	35.0	41.0	33.0	41.0
30	36.929420875876644	39.0	35.0	41.0	32.0	41.0
31	36.709983031589516	39.0	35.0	41.0	31.0	41.0
32	36.42492960775	39.0	35.0	41.0	30.0	41.0
33	36.052346917445355	39.0	35.0	41.0	29.0	41.0
34	35.60190060572374	39.0	35.0	41.0	24.0	41.0
35	35.35182678445327	39.0	35.0	41.0	23.0	41.0
36	35.21310209257098	39.0	35.0	41.0	22.0	41.0
37	35.1531358616844	39.0	35.0	41.0	22.0	41.0
38	35.053791298733266	39.0	35.0	41.0	21.0	41.0
39	34.957788009269436	39.0	35.0	41.0	21.0	41.0
40	34.8495214045747	39.0	35.0	41.0	20.0	41.0
41	34.720431511530194	39.0	34.0	41.0	18.0	41.0
42	34.6775828310592	39.0	34.0	41.0	18.0	41.0
43	34.53879375145621	38.0	34.0	41.0	18.0	41.0
44	34.395726032943756	38.0	34.0	41.0	18.0	41.0
45	34.337269469499084	38.0	34.0	40.0	18.0	41.0
46	34.2589353685044	38.0	34.0	40.0	18.0	41.0
47	34.22218195909972	38.0	34.0	40.0	18.0	41.0
48	34.11366499098872	38.0	34.0	40.0	18.0	41.0
49	34.032692490834485	38.0	34.0	40.0	18.0	41.0
50	33.91990047754725	38.0	34.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	7.0
11	12.0
12	5.0
13	11.0
14	28.0
15	72.0
16	189.0
17	420.0
18	1042.0
19	2228.0
20	4049.0
21	6807.0
22	10669.0
23	16553.0
24	25759.0
25	43038.0
26	68250.0
27	84870.0
28	82168.0
29	73314.0
30	70775.0
31	74410.0
32	86927.0
33	110082.0
34	180008.0
35	298892.0
36	207043.0
37	262828.0
38	469411.0
39	1159272.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.60189641303495	22.48736653024456	27.511585297558117	19.399151759162372
2	37.314031791362225	23.758919196704788	28.062594447801924	10.864454564131067
3	26.54079815617526	23.520445047925428	38.179372809694456	11.759383986204856
4	23.57977159429387	25.74718206391694	38.106300233652554	12.56674610813663
5	20.585982164301893	29.560432517775503	36.85331518897347	13.000270128949138
6	19.62049577945978	36.97118963950663	34.92997910244116	8.478335478592431
7	77.35247275800458	4.629686752241442	15.998701464386404	2.019139025367564
8	77.44587987467455	4.293582850225777	15.93823690248944	2.3223003726102425
9	70.8203235198461	7.758450813471468	18.165453082914016	3.2557725837684246
10	31.109002122099483	33.733535460863344	24.56433471312725	10.593127703909921
11	26.536126302952912	24.03165360244805	36.72465953869642	12.707560555902617
12	25.725559768875033	22.82934019656523	37.27767519000367	14.167424844556061
13	18.86281701968108	29.024157673848343	36.34318475442523	15.769840552045345
14	14.835619646460502	30.629178837942394	38.203660456895264	16.331541058701834
15	14.36223513437268	27.088153677616972	44.159015508755836	14.390595679254517
16	17.050677029396137	25.330997806025852	41.48635609224634	16.131969072331668
17	16.339477219624417	26.036597381486164	37.55292520902051	20.071000189868908
18	18.029370383924512	26.597668984824264	39.62381399315873	15.749146638092496
19	19.566170511861415	27.726041329130265	36.03454296397941	16.67324519502891
20	20.974195198413007	26.15294449538894	38.00597518048028	14.866885125717774
21	18.87404743608096	27.608945520800827	38.470165725008734	15.04684131810948
22	19.509119996550016	24.822843924763998	37.12682823692045	18.541207841765527
23	17.110392896866443	27.88431533092593	36.92210523289488	18.083186539312745
24	15.561254284778203	26.189690417849356	43.05768600714075	15.191369290231693
25	16.266135113588923	30.045736245135735	38.18979463611355	15.498334005161798
26	16.228969922249583	30.13878398848089	36.36681355053059	17.26543253873895
27	15.419840881470892	31.412552790444025	36.67470664654975	16.492899681535338
28	14.533506471415148	29.701336808872686	39.634116028469556	16.131040691242614
29	16.156226771755414	26.47491304662929	38.618227534824776	18.750632646790528
30	16.892043654275675	29.701486547758023	37.49207132602168	15.914398471944624
31	18.78713898703441	30.537957908998287	34.67191910746041	16.00298399650689
32	19.168523927974398	29.1389175555666	34.39169775745056	17.30086075900844
33	18.674565293041994	31.021105396409745	34.10192306655654	16.202406243991728
34	17.523043317063706	28.99151459684602	35.5008136811029	17.984628404987383
35	18.711910171043733	29.779081238136936	34.06212247083536	17.446886119983972
36	20.328431281531266	32.301103336002676	31.62652965758311	15.743935724882949
37	17.51873083716615	32.34605494937927	34.79904742110707	15.336166792347505
38	17.641247193144594	32.11623570817209	33.96245626875854	16.28006082992478
39	17.95896316004152	31.855809838803097	33.16524644325226	17.01998055790313
40	19.126267614533777	30.50798018415487	32.75328482192752	17.61246737938383
41	16.19482945639394	30.318740180872595	33.50482428740762	19.981606075325846
42	17.746064412876823	30.4161902474465	31.560614600260067	20.277130739416606
43	17.359258924287825	30.934256842917318	32.3276969620376	19.378787270757254
44	17.383247093717973	31.018739522021498	32.118002627019	19.480010757241523
45	17.110153314649914	33.826463413100235	29.92732273461538	19.13606053763447
46	17.727676477758088	33.25646737219636	31.80522804333803	17.210628106707524
47	17.92865600965037	30.88184823305121	33.4280381870095	17.76145757028893
48	17.36833310073893	30.057715355962273	34.47297602440864	18.10097551889016
49	17.362463336433926	31.481732155467295	32.88754070651598	18.268263801582798
50	16.478345061881093	31.760575907732097	33.918642670910465	17.842436359476345
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5442.0
1	10933.0
2	16424.0
3	59471.0
4	102518.0
5	70898.5
6	39279.0
7	39818.0
8	40357.0
9	41471.5
10	42586.0
11	42258.0
12	41930.0
13	40897.0
14	39864.0
15	38058.5
16	36253.0
17	35252.0
18	34251.0
19	34727.0
20	35203.0
21	37923.0
22	40643.0
23	47214.0
24	53785.0
25	58741.5
26	63698.0
27	79484.5
28	95271.0
29	110244.5
30	125218.0
31	143825.5
32	162433.0
33	186236.5
34	210040.0
35	209968.5
36	209897.0
37	220970.5
38	232044.0
39	230138.5
40	228233.0
41	222460.0
42	216687.0
43	211006.0
44	205325.0
45	195664.5
46	186004.0
47	180942.0
48	175880.0
49	164721.5
50	153563.0
51	133525.5
52	113488.0
53	101028.0
54	88568.0
55	79921.5
56	71275.0
57	67207.5
58	63140.0
59	58979.5
60	54819.0
61	49899.0
62	44979.0
63	39616.5
64	34254.0
65	29875.5
66	25497.0
67	21658.0
68	17819.0
69	14751.5
70	11684.0
71	10287.0
72	8890.0
73	7114.5
74	5339.0
75	4248.5
76	3158.0
77	2570.5
78	1983.0
79	1335.0
80	687.0
81	580.5
82	474.0
83	322.0
84	170.0
85	114.0
86	58.0
87	37.5
88	17.0
89	10.5
90	4.0
91	4.5
92	5.0
93	4.5
94	4.0
95	4.5
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3339146.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.83891972507785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.47845265212882	15.569277223139066
2	8.0404583708129	3.1902801634277465
3	3.0049617909741375	1.7884558724418624
4	1.627698434292742	1.2916711429827445
5	1.0300813620927682	1.0217850726428641
6	0.7137646581432121	0.8496191853300493
7	0.5564583271943209	0.7727672458491466
8	0.4386169683128828	0.6961349459533038
9	0.38202627280532053	0.6821089703149902
>10	4.0921198803420085	18.75968325400406
>50	0.8902584226790461	12.226807801866569
>100	0.6457981620608382	26.565154606088264
>500	0.08830479313405683	11.613030216084299
>1k	0.010694352109660861	2.8909823284251908
>5k	1.5277645870944085E-4	0.19037207893270944
>10k+	1.5277645870944085E-4	1.8918698925172408
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62419	1.8693102967046065	No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAA	6281	0.188101987753755	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3474	0.10403857752850579	No Hit
TTTCTAGGGATTATCAAACATTGATGGGGTTAATATTACAGTCTATGAAA	3418	0.1023615020127901	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04740733109603473	0.0
2	0.0	0.0	0.0	0.16022060730498158	0.0
3	0.0	0.0	0.0	0.23715644658843907	0.0
4	0.0	0.0	0.0	0.3732990411320739	0.0
5	0.0	0.0	0.0	0.6788262627629938	0.0
6	0.0	0.0	0.0	1.0103781026645735	0.0
7	0.0	0.0	0.0	1.2221687820778127	0.0
8	0.0	0.0	0.0	1.6818671600463113	0.0
9	0.0	0.0	0.0	1.8831761174863273	0.0
10	0.0	0.0	0.0	2.219609445049722	0.0
11	0.0	0.0	0.0	2.5743708121777247	0.0
12	0.0	0.0	0.0	2.8735790528476444	0.0
13	0.0	0.0	0.0	2.9914535033808045	0.0
14	0.0	0.0	0.0	3.0400886933365596	0.0
15	0.0	0.0	0.0	3.1153474571043014	0.0
16	0.0	0.0	0.0	3.269279031225349	0.0
17	0.0	0.0	0.0	3.447108931445346	0.0
18	0.0	0.0	0.0	3.6790544648242394	0.0
19	2.9947777066351694E-5	0.0	0.0	3.813639774960424	0.0
20	2.9947777066351694E-5	0.0	0.0	3.959335710388225	0.0
21	2.9947777066351694E-5	0.0	0.0	4.16106393670717	0.0
22	2.9947777066351694E-5	0.0	0.0	4.371806443923087	0.0
23	2.9947777066351694E-5	0.0	0.0	4.601326207359606	0.0
24	2.9947777066351694E-5	0.0	0.0	4.780743339764119	0.0
25	2.9947777066351694E-5	0.0	0.0	4.9424613359224185	0.0
26	2.9947777066351694E-5	0.0	0.0	5.101603823253011	0.0
27	2.9947777066351694E-5	0.0	0.0	5.264220252723301	0.0
28	5.989555413270339E-5	0.0	0.0	5.438306680810004	0.0
29	5.989555413270339E-5	0.0	0.0	5.632667753970626	0.0
30	5.989555413270339E-5	0.0	0.0	5.8728189782657	0.0
31	5.989555413270339E-5	0.0	0.0	6.080716446660314	0.0
32	5.989555413270339E-5	0.0	0.0	6.284630860705103	0.0
33	5.989555413270339E-5	0.0	0.0	6.482615614890753	0.0
34	5.989555413270339E-5	0.0	0.0	6.684134206770234	0.0
35	5.989555413270339E-5	0.0	0.0	6.933928615280673	0.0
36	5.989555413270339E-5	0.0	0.0	7.147186735770164	0.0
37	5.989555413270339E-5	0.0	0.0	7.371854959321935	0.0
38	8.984333119905509E-5	0.0	0.0	7.61479132688418	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGC	20	7.8586384E-4	44.000004	9
CGTTTTT	34275	0.0	42.20277	1
TACGTTG	80	0.0	41.250004	1
TACGGGA	1290	0.0	41.100773	4
TGCTACG	265	0.0	40.67925	1
TATACGG	430	0.0	39.90698	2
CGACGGT	290	0.0	39.448273	28
CTAAGCG	90	0.0	39.11111	1
GCCCTAC	2915	0.0	38.867924	26
GCCCTAG	2945	0.0	38.8455	20
CGAATAT	425	0.0	38.823532	14
TAACGTT	85	0.0	38.82353	12
CGGTCTA	290	0.0	38.689655	31
GCGTAAG	165	0.0	38.666668	1
CAATTAT	5875	0.0	38.645107	44
ATTAGCG	120	0.0	38.5	1
ATACTCG	355	0.0	38.422535	44
CCCTAGC	2995	0.0	38.196995	21
ACGGGAC	800	0.0	37.95	5
AGTACGG	470	0.0	37.91489	2
>>END_MODULE