FastQCFastQC Report
Thu 26 May 2016
SRR1546184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546184_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3415206
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT631821.8500201744784943No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAA62590.1832685934611265No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35270.10327341893871117No Hit
TTTCTAGGGATTATCAAACATTGATGGGGTTAATATTACAGTCTATGAAA34940.10230715218935549No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGATA207.858665E-444.020
CCGTCGA207.858665E-444.014
CTCGCTA207.858665E-444.033
TCGAGTA207.858665E-444.026
ATCGCGC207.858665E-444.010
CGTTTTT345250.042.209411
CACGTAT1250.040.4830
TAATGCG1250.040.481
TATACGG3650.040.3835642
CTAGACG603.6379788E-1240.3333321
TACGGGA11650.040.0343364
GACTACG557.8216544E-1140.01
GTCGATA501.3496901E-939.65
AATGCGG4100.039.170732
GCGCGTA452.3532266E-839.1111126
CCCTAGC28450.038.97363721
CTATGCG850.038.8235321
GCCCTAC28300.038.7915226
GCCCTAG28700.038.78745720
AGGGCGA13950.038.637996
Produced by FastQC (version 0.11.5)