##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546184_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3415206
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86114454003653	31.0	31.0	34.0	30.0	34.0
2	32.25917909490672	33.0	31.0	34.0	30.0	34.0
3	32.29402677320197	34.0	31.0	34.0	30.0	34.0
4	35.982436784193986	37.0	35.0	37.0	35.0	37.0
5	35.90936417891044	37.0	35.0	37.0	35.0	37.0
6	35.97799488522801	37.0	35.0	37.0	35.0	37.0
7	36.18101836316755	37.0	35.0	37.0	35.0	37.0
8	36.07078284589568	37.0	35.0	37.0	35.0	37.0
9	37.766496955088506	39.0	38.0	39.0	35.0	39.0
10	37.520477534883696	39.0	37.0	39.0	35.0	39.0
11	37.40361869825715	39.0	37.0	39.0	34.0	39.0
12	37.1681160667907	39.0	37.0	39.0	34.0	39.0
13	37.09164337378184	39.0	37.0	39.0	33.0	39.0
14	38.31959887631961	40.0	38.0	41.0	33.0	41.0
15	38.40306324128032	40.0	37.0	41.0	33.0	41.0
16	38.44593825379787	40.0	37.0	41.0	34.0	41.0
17	38.347689421955806	40.0	37.0	41.0	34.0	41.0
18	38.16288095066594	39.0	37.0	41.0	34.0	41.0
19	37.971114187548274	40.0	37.0	41.0	34.0	41.0
20	37.737046022992466	40.0	35.0	41.0	34.0	41.0
21	37.633408643578164	39.0	35.0	41.0	33.0	41.0
22	37.551753832711704	39.0	35.0	41.0	33.0	41.0
23	37.49149216767598	39.0	35.0	41.0	33.0	41.0
24	37.426835745779314	39.0	35.0	41.0	33.0	41.0
25	37.41456152278955	39.0	35.0	41.0	33.0	41.0
26	37.37188708382452	39.0	35.0	41.0	33.0	41.0
27	37.26767667894704	39.0	35.0	41.0	33.0	41.0
28	37.18910513743534	39.0	35.0	41.0	33.0	41.0
29	37.097404373264744	39.0	35.0	41.0	33.0	41.0
30	36.91297538128008	39.0	35.0	41.0	32.0	41.0
31	36.694450056599806	39.0	35.0	41.0	31.0	41.0
32	36.41509267669359	39.0	35.0	41.0	30.0	41.0
33	36.02121511850237	39.0	35.0	41.0	29.0	41.0
34	35.64810087590617	39.0	35.0	41.0	25.0	41.0
35	35.380319078849126	39.0	35.0	41.0	23.0	41.0
36	35.230259316714715	39.0	35.0	41.0	23.0	41.0
37	35.09729779111421	39.0	35.0	41.0	22.0	41.0
38	35.004670289288555	39.0	35.0	41.0	21.0	41.0
39	34.91227674113948	39.0	35.0	41.0	21.0	41.0
40	34.823970208532074	39.0	35.0	41.0	20.0	41.0
41	34.71589707912202	39.0	34.0	41.0	18.0	41.0
42	34.65599849613757	39.0	34.0	41.0	18.0	41.0
43	34.544645037517505	38.0	34.0	41.0	18.0	41.0
44	34.41609437322375	38.0	34.0	41.0	18.0	41.0
45	34.346156571521604	38.0	34.0	40.0	18.0	41.0
46	34.27349009108089	38.0	34.0	40.0	18.0	41.0
47	34.20842666591708	38.0	34.0	40.0	18.0	41.0
48	34.12221488249904	38.0	34.0	40.0	18.0	41.0
49	34.0365128779933	38.0	34.0	40.0	18.0	41.0
50	33.895987533402085	38.0	34.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	6.0
11	5.0
12	10.0
13	8.0
14	31.0
15	70.0
16	215.0
17	536.0
18	1100.0
19	2320.0
20	4275.0
21	6989.0
22	10914.0
23	17315.0
24	26152.0
25	43458.0
26	69584.0
27	86206.0
28	84233.0
29	75632.0
30	72982.0
31	76988.0
32	90045.0
33	113081.0
34	187827.0
35	302613.0
36	213297.0
37	270636.0
38	481434.0
39	1177239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.7344271472936	22.55395428562728	27.460949646961268	19.25066892011785
2	37.297691559454975	23.760206558550202	28.03599548607024	10.90610639592458
3	26.543669693716865	23.56616262679323	38.17239721410656	11.717770465383346
4	23.627857294699062	25.756689347582544	38.047075344796184	12.568378012922206
5	20.593808982532824	29.586414406627306	36.79441298709361	13.02536362374627
6	19.6715805722993	36.959791005286355	34.84595072742317	8.522677694991165
7	77.38502450511038	4.650554022217108	15.96571334203559	1.998708130636922
8	77.41717483513439	4.315640110728313	15.91186593136695	2.3553191227703394
9	70.75330155779768	7.779179352577853	18.167220366794858	3.300298722829604
10	31.14564685117091	33.65296266169596	24.601590650754304	10.59979983637883
11	26.5475054799037	24.050408672273356	36.66364488701414	12.738440960808806
12	25.729399632115896	22.83253191754758	37.23825151396431	14.199816936372214
13	18.942781196800425	29.008821136997298	36.31107464674166	15.73732301946061
14	14.897988583997568	30.617303904947462	38.14176948623304	16.34293802482193
15	14.421911884671085	27.06665425160298	44.12319491122937	14.388238952496572
16	17.059498021495628	25.351589333117825	41.502269555628565	16.08664308975798
17	16.421381316383258	25.97749594021561	37.521924006926675	20.079198736474464
18	18.067431364315944	26.55910068089597	39.59313142457585	15.780336530212233
19	19.671756257162816	27.672298537774882	36.044033654192454	16.611911550869845
20	21.03682764670711	26.136724988185193	37.915487382020295	14.910959983087405
21	18.927320928810737	27.538983007174384	38.473579631799666	15.060116432215217
22	19.409605159981567	24.84063918838278	37.16250205697694	18.587253594658712
23	17.180134961112156	27.807048828094118	36.91162407187151	18.10119213892222
24	15.568372742376301	26.234903546081846	42.986806652365914	15.20991705917593
25	16.261508090580772	29.942820433086613	38.24173417357547	15.55393730275714
26	16.218992353609124	30.150714188251015	36.330487824160535	17.299805633979325
27	15.468437335844456	31.375413371843457	36.67866594284503	16.477483349467057
28	14.489843365231847	29.70913028379547	39.586836050299745	16.214190300672932
29	16.1616312456701	26.508415597770675	38.61869532906653	18.711257827492688
30	16.94667905830571	29.65762533797376	37.473434984595364	15.922260619125172
31	18.773860200526705	30.536283902054517	34.66558093421012	16.02427496320866
32	19.19201945651302	29.140877592742576	34.37057676755077	17.296526183193635
33	18.722618782000268	31.000179784177	34.01349728244797	16.263704151374764
34	17.51809407690195	29.078743712677944	35.36896456611988	18.034197644300225
35	18.743759527243746	29.73694705385268	33.92577197393071	17.593521444972865
36	20.423072576002735	32.230559445023225	31.570628536023886	15.775739442950146
37	17.596625210895038	32.31239931061259	34.7156511203131	15.375324358179274
38	17.6573828928621	32.069163617070245	33.92070639369924	16.352747096368418
39	18.052029657947426	31.80279608316453	33.10339112779727	17.041783131090774
40	19.167218609946225	30.44838290867374	32.757086980990316	17.627311500389727
41	16.21714766254217	30.332870110909855	33.43198038419937	20.0180018423486
42	17.808471875488625	30.45500037186629	31.461147585240834	20.27538016740425
43	17.415347712553796	30.87787383835704	32.276647440886435	19.430131008202725
44	17.40389891561446	31.00340067334152	32.09630692848396	19.496393482560055
45	17.16930106119514	33.77389240941835	29.843002149797115	19.2138043795894
46	17.764521378798236	33.25796452688359	31.74496648225612	17.232547612062056
47	17.919211901126904	30.916202419414816	33.309323068652375	17.85526261080591
48	17.34601075308488	30.13876761753171	34.34314064803119	18.172080981352224
49	17.383080259287436	31.45414947151065	32.82709739910272	18.3356728700992
50	16.52471329694314	31.708424030644128	33.87007987219512	17.896782800217615
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5511.0
1	11383.5
2	17256.0
3	60693.0
4	104130.0
5	71854.0
6	39578.0
7	40272.0
8	40966.0
9	42209.0
10	43452.0
11	43017.5
12	42583.0
13	41710.0
14	40837.0
15	38865.0
16	36893.0
17	35778.5
18	34664.0
19	34875.0
20	35086.0
21	38394.0
22	41702.0
23	48150.5
24	54599.0
25	59931.5
26	65264.0
27	81078.0
28	96892.0
29	112140.5
30	127389.0
31	146112.5
32	164836.0
33	188910.0
34	212984.0
35	213238.0
36	213492.0
37	224948.5
38	236405.0
39	235090.0
40	233775.0
41	227436.5
42	221098.0
43	216163.5
44	211229.0
45	201383.5
46	191538.0
47	187180.0
48	182822.0
49	171319.5
50	159817.0
51	138760.5
52	117704.0
53	104854.0
54	92004.0
55	82763.0
56	73522.0
57	69206.5
58	64891.0
59	60392.5
60	55894.0
61	50852.5
62	45811.0
63	40090.5
64	34370.0
65	29952.0
66	25534.0
67	21854.0
68	18174.0
69	14887.0
70	11600.0
71	10210.0
72	8820.0
73	7158.0
74	5496.0
75	4297.5
76	3099.0
77	2536.0
78	1973.0
79	1360.5
80	748.0
81	609.5
82	471.0
83	328.5
84	186.0
85	123.0
86	60.0
87	45.0
88	30.0
89	19.0
90	8.0
91	5.5
92	3.0
93	5.5
94	8.0
95	5.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3415206.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.835458100630838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.44335908785702	15.55959962459927
2	8.012650733291878	3.1786919579039994
3	3.038475009529891	1.8080863122403212
4	1.5811660006078236	1.2545260782079408
5	1.0391385102745745	1.0305894190651637
6	0.7726972422809074	0.9196082264241561
7	0.5524163466834081	0.767020190911961
8	0.4556507667076152	0.7230433353239374
9	0.39730984156642507	0.7092740443823192
>10	4.073027636371407	18.591580348298788
>50	0.8868276712027633	12.161112836929426
>100	0.6469090232657724	26.503396809800744
>500	0.08827370393314435	11.583285474071218
>1k	0.011799699848931307	3.1528642891695187
>5k	1.493632892269786E-4	0.18543472111100476
>10k+	1.493632892269786E-4	1.8718863315602339
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63182	1.8500201744784943	No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAA	6259	0.1832685934611265	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3527	0.10327341893871117	No Hit
TTTCTAGGGATTATCAAACATTGATGGGGTTAATATTACAGTCTATGAAA	3494	0.10230715218935549	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04904535773244718	0.0
2	0.0	0.0	0.0	0.1653487373821667	0.0
3	0.0	0.0	0.0	0.24370418651173603	0.0
4	0.0	0.0	0.0	0.38515978245528965	0.0
5	0.0	0.0	0.0	0.6984644557312208	0.0
6	0.0	0.0	0.0	1.0408156931089956	0.0
7	0.0	0.0	0.0	1.2555025963294748	0.0
8	0.0	0.0	0.0	1.7281241600067463	0.0
9	0.0	0.0	0.0	1.940819968107341	0.0
10	0.0	0.0	0.0	2.289905791920019	0.0
11	0.0	0.0	0.0	2.6627383531183773	0.0
12	0.0	0.0	0.0	2.9776827517871545	0.0
13	0.0	0.0	0.0	3.102741093802248	0.0
14	0.0	0.0	0.0	3.154128916381618	0.0
15	0.0	0.0	0.0	3.231371694708899	0.0
16	0.0	0.0	0.0	3.3937045086006523	0.0
17	0.0	0.0	0.0	3.5829463874214325	0.0
18	0.0	0.0	0.0	3.8285831074318795	0.0
19	0.0	0.0	0.0	3.9673155879908855	0.0
20	0.0	0.0	0.0	4.126017581369909	0.0
21	0.0	0.0	0.0	4.3422563675514745	0.0
22	0.0	0.0	0.0	4.567777170688972	0.0
23	0.0	0.0	0.0	4.8057715991363334	0.0
24	0.0	0.0	0.0	4.995540532547671	0.0
25	0.0	0.0	0.0	5.162763241807376	0.0
26	0.0	0.0	0.0	5.329019684317725	0.0
27	0.0	0.0	0.0	5.499434001931363	0.0
28	0.0	0.0	0.0	5.6796866719020755	0.0
29	0.0	0.0	0.0	5.883539675205537	0.0
30	0.0	0.0	0.0	6.136115947324993	0.0
31	0.0	0.0	0.0	6.36087544938724	0.0
32	0.0	0.0	0.0	6.5676272529387685	0.0
33	0.0	0.0	0.0	6.779444636721768	0.0
34	0.0	0.0	0.0	6.9995192090901694	0.0
35	0.0	0.0	0.0	7.262665853831365	0.0
36	0.0	0.0	0.0	7.486546931576016	0.0
37	0.0	0.0	0.0	7.716401294680321	0.0
38	0.0	0.0	0.0	7.96768921113397	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGATA	20	7.858665E-4	44.0	20
CCGTCGA	20	7.858665E-4	44.0	14
CTCGCTA	20	7.858665E-4	44.0	33
TCGAGTA	20	7.858665E-4	44.0	26
ATCGCGC	20	7.858665E-4	44.0	10
CGTTTTT	34525	0.0	42.20941	1
CACGTAT	125	0.0	40.48	30
TAATGCG	125	0.0	40.48	1
TATACGG	365	0.0	40.383564	2
CTAGACG	60	3.6379788E-12	40.333332	1
TACGGGA	1165	0.0	40.034336	4
GACTACG	55	7.8216544E-11	40.0	1
GTCGATA	50	1.3496901E-9	39.6	5
AATGCGG	410	0.0	39.17073	2
GCGCGTA	45	2.3532266E-8	39.11111	26
CCCTAGC	2845	0.0	38.973637	21
CTATGCG	85	0.0	38.823532	1
GCCCTAC	2830	0.0	38.79152	26
GCCCTAG	2870	0.0	38.787457	20
AGGGCGA	1395	0.0	38.63799	6
>>END_MODULE