FastQCFastQC Report
Thu 26 May 2016
SRR1546183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546183_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1694345
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210901.2447287890010594No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30990.18290253755876165No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA207.8575395E-444.00000444
CGACGAA650.044.00000421
CTAACGG408.3164196E-944.0000042
TAGGCGA207.8575395E-444.00000417
ATCGGGT650.044.0000044
ACGACAG650.044.0000041
CGAAGTT408.3164196E-944.00000421
GTACGAT207.8575395E-444.00000424
AGTCCGT254.4437365E-544.044
ACCGGTC254.4437365E-544.034
TCCGCAT254.4437365E-544.023
ACCGCTT254.4437365E-544.013
TGCGGTA600.044.04
TACGCGG502.7284841E-1144.02
TATCTCG302.5284298E-644.01
GCGATAC750.044.09
CGGTCTA454.802132E-1044.031
CGATACT454.802132E-1044.010
AGTCGTA454.802132E-1044.033
ACGGTCC302.5284298E-644.033
Produced by FastQC (version 0.11.5)