##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546183_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1694345
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85654043302869	31.0	31.0	34.0	30.0	34.0
2	32.21391334114363	33.0	31.0	34.0	30.0	34.0
3	32.15767804077682	33.0	31.0	34.0	30.0	34.0
4	35.90094756380784	37.0	35.0	37.0	35.0	37.0
5	35.88987071700274	37.0	35.0	37.0	35.0	37.0
6	35.93242226347054	37.0	35.0	37.0	35.0	37.0
7	36.154659765278026	37.0	35.0	37.0	35.0	37.0
8	36.05146059391682	37.0	35.0	37.0	35.0	37.0
9	37.78746418232414	39.0	38.0	39.0	35.0	39.0
10	37.483811148260834	39.0	37.0	39.0	35.0	39.0
11	37.33112028542003	39.0	37.0	39.0	34.0	39.0
12	36.82479188122844	39.0	35.0	39.0	33.0	39.0
13	36.64111205214994	39.0	35.0	39.0	33.0	39.0
14	37.81944055077331	40.0	35.0	41.0	33.0	41.0
15	37.979783338104106	40.0	35.0	41.0	33.0	41.0
16	38.06132399245726	40.0	35.0	41.0	34.0	41.0
17	37.97770701952672	40.0	35.0	41.0	34.0	41.0
18	37.81237233267133	39.0	36.0	41.0	34.0	41.0
19	37.62028925632029	39.0	36.0	41.0	34.0	41.0
20	37.35133930811021	39.0	35.0	41.0	34.0	41.0
21	37.253878047269005	39.0	35.0	41.0	33.0	41.0
22	37.179187237546074	39.0	35.0	41.0	33.0	41.0
23	37.11660907312265	38.0	35.0	41.0	33.0	41.0
24	37.060933871200966	38.0	35.0	41.0	33.0	41.0
25	37.05132307764948	38.0	35.0	41.0	33.0	41.0
26	36.988737830843185	38.0	35.0	41.0	33.0	41.0
27	36.929397495787455	38.0	35.0	41.0	33.0	41.0
28	36.88360811995196	38.0	35.0	41.0	33.0	41.0
29	36.81534162168862	38.0	35.0	41.0	32.0	41.0
30	36.627301995756476	38.0	35.0	41.0	32.0	41.0
31	36.3569025198528	38.0	35.0	41.0	31.0	41.0
32	36.03748705251882	38.0	35.0	41.0	30.0	41.0
33	35.64078331154517	38.0	35.0	41.0	28.0	41.0
34	35.19134296734136	38.0	35.0	41.0	24.0	41.0
35	34.952642466557876	38.0	35.0	41.0	23.0	41.0
36	34.78647441931838	38.0	35.0	41.0	22.0	41.0
37	34.75049473395324	38.0	35.0	41.0	22.0	41.0
38	34.72229150497685	38.0	34.0	41.0	22.0	41.0
39	34.636818947734966	38.0	34.0	41.0	21.0	41.0
40	34.52582915521927	38.0	34.0	40.0	21.0	41.0
41	34.416393945743046	38.0	34.0	40.0	19.0	41.0
42	34.3422171989766	38.0	34.0	40.0	18.0	41.0
43	34.22785914320873	38.0	34.0	40.0	18.0	41.0
44	34.100025673637894	38.0	34.0	40.0	18.0	41.0
45	34.061815037669426	38.0	34.0	40.0	18.0	41.0
46	33.99109213294813	37.0	34.0	40.0	18.0	41.0
47	33.937248907394896	37.0	34.0	40.0	18.0	41.0
48	33.86523464819739	37.0	34.0	40.0	18.0	41.0
49	33.78809274380365	37.0	34.0	40.0	18.0	41.0
50	33.628160734679184	37.0	33.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	1.0
11	8.0
12	7.0
13	14.0
14	16.0
15	37.0
16	91.0
17	222.0
18	528.0
19	1085.0
20	1954.0
21	3252.0
22	5384.0
23	8479.0
24	13582.0
25	23129.0
26	37170.0
27	46316.0
28	43276.0
29	37555.0
30	36399.0
31	39540.0
32	47216.0
33	61256.0
34	108332.0
35	222789.0
36	97115.0
37	123083.0
38	212294.0
39	524213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.21886923855531	24.21856233529771	28.458489858913033	19.104078567233945
2	33.508464923023354	25.12180223036041	29.75261826841641	11.617114578199836
3	24.71503737432459	24.715863652325826	39.24590328415996	11.323195689189628
4	22.15457890807362	26.670011125243086	39.46870324520685	11.706706721476442
5	19.621092516577203	30.286157777784332	38.39164987059897	11.7010998350395
6	19.09534362836376	37.52423502887547	35.01547795755882	8.36494338520195
7	78.13414623350026	4.792884566012235	15.158896210630065	1.9140729898574378
8	79.34558782302305	3.870581257063939	14.467478583169308	2.3163523367436976
9	73.93305377594291	6.0883114123746935	16.694828975208708	3.2838058364736815
10	37.0914424158008	33.14478456276614	21.2312722615524	8.532500759880662
11	32.619389793696094	21.484231369644316	33.16154620221974	12.734832634439858
12	30.327058538845396	20.683922105592426	35.8851355538571	13.103883801705082
13	16.226978566938847	36.64501621570577	33.209706405720205	13.918298811635173
14	12.08325341061	36.99305631379678	35.77500450026411	15.14868577532911
15	11.116449129309556	24.593515488286034	50.84749563990805	13.442539742496361
16	12.640282823155852	22.539211317647823	45.972868571630926	18.8476372875654
17	12.792908173955128	23.110346476071875	36.29567768075569	27.80106766921731
18	15.393500143123154	25.749360372297257	42.263057405664135	16.594082078915452
19	19.551567124759124	26.921081597903612	34.47078369517424	19.05656758216302
20	22.21566446030767	23.6646609751851	37.191008914949435	16.928665649557793
21	17.057092858892375	27.97854037991082	37.33401402902006	17.63035273217674
22	19.227961247561744	24.62373365518829	32.232042470689265	23.916262626560705
23	14.124160073656784	28.72880080503085	34.84449743116072	22.30254169015165
24	13.171520558091771	24.85875072668199	47.485252413174415	14.484476302051824
25	12.673511002776886	29.64349055239635	41.73866597416701	15.944332470659752
26	14.265276552296019	31.261224839097114	35.53083935089961	18.942659257707255
27	12.935677208596832	33.97938436386922	34.34530747870121	18.739630948832733
28	11.671471866709554	30.185646960920003	40.21713405475273	17.92574711761772
29	12.290295069776226	25.31981385137029	39.001797154652685	23.3880939242008
30	13.938483602808166	31.975424131449024	36.14978059368074	17.936311672062068
31	18.91728071909794	32.50270753595047	32.33461898255668	16.245392762394907
32	20.19388023100372	27.268649537136767	33.08529254667733	19.45217768518218
33	20.023076764177308	31.327268059338564	29.82975722181728	18.819897954666846
34	15.349648389200546	28.331892265152607	33.1782488218161	23.140210523830742
35	16.1243430352142	29.169561098831704	34.08060341902033	20.625492446933773
36	22.448674856655522	30.957567673643798	29.936347083976404	16.657410385724276
37	14.47326252917794	33.13103293603133	35.411147080435214	16.984557454355517
38	15.397513493414861	31.07070873995556	34.497755769928794	19.03402199670079
39	16.247871596398607	33.07059660222682	32.57783981420549	18.103691987169082
40	18.06798497354435	28.63460511289023	32.78812756551942	20.509282348046
41	13.8844214135846	29.308493842753396	33.50563196987626	23.30145277378574
42	15.786690431995845	30.13984755170877	30.849207215767745	23.22425480052764
43	16.2339429100921	28.726440010741616	32.97451227465481	22.065104804511478
44	15.028226246720708	30.337564073432503	31.88329413431149	22.750915545535292
45	14.69806916537069	36.04047581808899	28.562895986354608	20.698559030185706
46	18.768255579589756	33.48946052899498	30.688378104813363	17.053905786601902
47	16.10439432347013	31.14749357421304	32.40862988352431	20.33948221879251
48	16.334571766670898	28.148104429735383	34.47155095331824	21.045772850275476
49	17.73552611776232	27.36408464627924	35.39651015584193	19.503879080116505
50	16.58103869046741	31.529174990925696	32.34435725899979	19.545429059607105
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4518.0
1	5668.5
2	6819.0
3	21273.5
4	35728.0
5	26578.5
6	17429.0
7	18737.0
8	20045.0
9	21604.5
10	23164.0
11	22930.0
12	22696.0
13	21638.5
14	20581.0
15	19165.0
16	17749.0
17	16307.0
18	14865.0
19	14223.0
20	13581.0
21	14901.0
22	16221.0
23	17485.5
24	18750.0
25	21986.5
26	25223.0
27	28151.0
28	31079.0
29	40423.5
30	49768.0
31	62700.5
32	75633.0
33	88643.5
34	101654.0
35	107937.5
36	114221.0
37	110030.5
38	105840.0
39	114942.5
40	124045.0
41	134613.5
42	145182.0
43	142098.5
44	139015.0
45	136102.0
46	133189.0
47	122548.5
48	111908.0
49	102041.5
50	92175.0
51	75890.0
52	59605.0
53	50115.0
54	40625.0
55	33687.5
56	26750.0
57	24251.0
58	21752.0
59	20075.5
60	18399.0
61	16707.5
62	15016.0
63	12695.0
64	10374.0
65	8726.0
66	7078.0
67	6138.0
68	5198.0
69	4255.5
70	3313.0
71	2760.0
72	2207.0
73	1825.0
74	1443.0
75	1045.5
76	648.0
77	559.0
78	470.0
79	343.5
80	217.0
81	153.0
82	89.0
83	67.5
84	46.0
85	31.5
86	17.0
87	13.5
88	10.0
89	9.5
90	9.0
91	4.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1694345.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.458931776133355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.31774952093461	18.13791811095772
2	8.930021811795235	4.189775448845744
3	3.4601682451982216	2.435155523941445
4	1.807116168199389	1.6957205960534798
5	1.1433601489539413	1.3411003864930093
6	0.7883265002825534	1.1095978552467836
7	0.5940752225938426	0.9755459081704131
8	0.47496516569437536	0.891374033445138
9	0.3742348762828926	0.7901235387873076
>10	3.8150523144383963	19.855536068128483
>50	0.7204228753612079	11.70224829037948
>100	0.5107854065197055	24.572329370529435
>500	0.056894414058574316	9.095295509526164
>1k	0.006574465624546365	1.9572372647971392
>5k	0.0	0.0
>10k+	2.5286406248255255E-4	1.2510420946983383
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21090	1.2447287890010594	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3099	0.18290253755876165	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09366451342554202	0.0
2	0.0	0.0	0.0	0.3615556453968938	0.0
3	0.0	0.0	0.0	0.5112890231918529	0.0
4	0.0	0.0	0.0	0.8070965476334513	0.0
5	0.0	0.0	0.0	1.4383139207186257	0.0
6	0.0	0.0	0.0	2.0059078877088194	0.0
7	0.0	0.0	0.0	2.3690570692509496	0.0
8	0.0	0.0	0.0	3.20790630007466	0.0
9	0.0	0.0	0.0	3.510087969097203	0.0
10	0.0	0.0	0.0	4.0264527000109185	0.0
11	0.0	0.0	0.0	4.5974698187205085	0.0
12	0.0	0.0	0.0	5.114365728349303	0.0
13	5.9019857230965356E-5	0.0	0.0	5.304055549489626	0.0
14	5.9019857230965356E-5	0.0	0.0	5.374466239166168	0.0
15	5.9019857230965356E-5	0.0	0.0	5.491738695484096	0.0
16	5.9019857230965356E-5	0.0	0.0	5.747353697151406	0.0
17	5.9019857230965356E-5	0.0	0.0	6.074146646639262	0.0
18	5.9019857230965356E-5	0.0	0.0	6.511306729149022	0.0
19	5.9019857230965356E-5	0.0	0.0	6.720945262033411	0.0
20	5.9019857230965356E-5	0.0	0.0	6.978980077847192	0.0
21	5.9019857230965356E-5	0.0	0.0	7.320823090928943	0.0
22	5.9019857230965356E-5	0.0	0.0	7.671637122309801	0.0
23	5.9019857230965356E-5	0.0	0.0	8.09274380365274	0.0
24	5.9019857230965356E-5	0.0	0.0	8.384656017517093	0.0
25	5.9019857230965356E-5	0.0	0.0	8.633188636316689	0.0
26	5.9019857230965356E-5	0.0	0.0	8.904561939864667	0.0
27	5.9019857230965356E-5	0.0	0.0	9.15539633309627	0.0
28	5.9019857230965356E-5	0.0	0.0	9.450849738394483	0.0
29	5.9019857230965356E-5	0.0	0.0	9.74051919768406	0.0
30	5.9019857230965356E-5	0.0	0.0	10.146457775718641	0.0
31	5.9019857230965356E-5	0.0	0.0	10.524125842139588	0.0
32	5.9019857230965356E-5	0.0	0.0	10.843482289616341	0.0
33	5.9019857230965356E-5	0.0	0.0	11.146313177068425	0.0
34	5.9019857230965356E-5	0.0	0.0	11.472574947841201	0.0
35	5.9019857230965356E-5	0.0	0.0	11.897222820617998	0.0
36	5.9019857230965356E-5	0.0	0.0	12.233990125977884	0.0
37	5.9019857230965356E-5	0.0	0.0	12.57300018591255	0.0
38	5.9019857230965356E-5	0.0	0.0	12.878959125797875	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCTA	20	7.8575395E-4	44.000004	44
CGACGAA	65	0.0	44.000004	21
CTAACGG	40	8.3164196E-9	44.000004	2
TAGGCGA	20	7.8575395E-4	44.000004	17
ATCGGGT	65	0.0	44.000004	4
ACGACAG	65	0.0	44.000004	1
CGAAGTT	40	8.3164196E-9	44.000004	21
GTACGAT	20	7.8575395E-4	44.000004	24
AGTCCGT	25	4.4437365E-5	44.0	44
ACCGGTC	25	4.4437365E-5	44.0	34
TCCGCAT	25	4.4437365E-5	44.0	23
ACCGCTT	25	4.4437365E-5	44.0	13
TGCGGTA	60	0.0	44.0	4
TACGCGG	50	2.7284841E-11	44.0	2
TATCTCG	30	2.5284298E-6	44.0	1
GCGATAC	75	0.0	44.0	9
CGGTCTA	45	4.802132E-10	44.0	31
CGATACT	45	4.802132E-10	44.0	10
AGTCGTA	45	4.802132E-10	44.0	33
ACGGTCC	30	2.5284298E-6	44.0	33
>>END_MODULE