FastQCFastQC Report
Thu 26 May 2016
SRR1546182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546182_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1746165
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218971.25400520569362No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31720.18165522731242467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACT302.5284753E-644.00000411
TCGAGTA302.5284753E-644.00000439
AGGTACG207.8576076E-444.01
GTACCGG207.8576076E-444.02
CACGGTC254.4437937E-544.032
ACGTTAG900.044.01
CTATACG502.7284841E-1144.01
TCGGGTA254.4437937E-544.05
AAGCGAC207.8576076E-444.015
TACGGAC800.044.04
TCGATAG700.044.01
CTGCGTA207.8576076E-444.020
TACGAAG551.8189894E-1244.01
GTGTCGA207.8576076E-444.042
TCGCTAG254.4437937E-544.014
CATACGT351.4466605E-744.013
GCGATCG408.3164196E-944.09
ACGTACT750.044.039
GCGTATC351.4466605E-744.09
CCCATAG351.4466605E-744.025
Produced by FastQC (version 0.11.5)