##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546182_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1746165
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79539734217557	31.0	31.0	34.0	30.0	34.0
2	32.18563022394791	33.0	31.0	34.0	30.0	34.0
3	32.24725269376032	33.0	31.0	34.0	30.0	34.0
4	35.93818224509138	37.0	35.0	37.0	35.0	37.0
5	35.84873365346345	37.0	35.0	37.0	35.0	37.0
6	35.920367777386446	37.0	35.0	37.0	35.0	37.0
7	36.13468772996824	37.0	35.0	37.0	35.0	37.0
8	36.036172412114546	37.0	35.0	37.0	35.0	37.0
9	37.76364032035919	39.0	38.0	39.0	35.0	39.0
10	37.48659548209935	39.0	37.0	39.0	35.0	39.0
11	37.33555706362228	39.0	37.0	39.0	34.0	39.0
12	36.81477179991582	39.0	35.0	39.0	33.0	39.0
13	36.595310866957014	39.0	35.0	39.0	33.0	39.0
14	37.76119496152998	40.0	35.0	41.0	33.0	41.0
15	37.95331139955273	40.0	35.0	41.0	33.0	41.0
16	38.02443182631653	40.0	35.0	41.0	34.0	41.0
17	37.94690879727861	40.0	35.0	41.0	34.0	41.0
18	37.79752600699246	39.0	36.0	41.0	34.0	41.0
19	37.613713480684815	39.0	36.0	41.0	34.0	41.0
20	37.345432991727584	39.0	35.0	41.0	34.0	41.0
21	37.24488579257974	39.0	35.0	41.0	33.0	41.0
22	37.17972642906025	39.0	35.0	41.0	33.0	41.0
23	37.10567672585351	38.0	35.0	41.0	33.0	41.0
24	37.04968946233603	38.0	35.0	41.0	33.0	41.0
25	37.03355467553181	38.0	35.0	41.0	33.0	41.0
26	36.99319136507718	38.0	35.0	41.0	33.0	41.0
27	36.92046685164346	38.0	35.0	41.0	33.0	41.0
28	36.87066686137908	38.0	35.0	41.0	33.0	41.0
29	36.812851591917145	38.0	35.0	41.0	32.0	41.0
30	36.625645915477634	38.0	35.0	41.0	32.0	41.0
31	36.36214790698474	38.0	35.0	41.0	31.0	41.0
32	36.049467261112206	38.0	35.0	41.0	30.0	41.0
33	35.64857673816621	38.0	35.0	41.0	28.0	41.0
34	35.27504789066325	38.0	35.0	41.0	25.0	41.0
35	34.99353153911572	38.0	35.0	41.0	23.0	41.0
36	34.82159761534563	38.0	35.0	41.0	22.0	41.0
37	34.71583842305853	38.0	34.0	41.0	22.0	41.0
38	34.701176578387496	38.0	34.0	41.0	22.0	41.0
39	34.62646657102851	38.0	34.0	41.0	21.0	41.0
40	34.53316553704833	38.0	34.0	40.0	21.0	41.0
41	34.45183645302706	38.0	34.0	40.0	19.0	41.0
42	34.36262266166141	38.0	34.0	40.0	18.0	41.0
43	34.27544934184341	38.0	34.0	40.0	18.0	41.0
44	34.14663390916666	38.0	34.0	40.0	18.0	41.0
45	34.09987945010924	38.0	34.0	40.0	18.0	41.0
46	34.03550294502524	37.0	34.0	40.0	18.0	41.0
47	33.96263926948484	37.0	34.0	40.0	18.0	41.0
48	33.90389682532865	37.0	34.0	40.0	18.0	41.0
49	33.82558177491818	37.0	34.0	40.0	18.0	41.0
50	33.643573201845186	37.0	33.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	7.0
12	5.0
13	10.0
14	10.0
15	41.0
16	87.0
17	253.0
18	514.0
19	1173.0
20	2067.0
21	3399.0
22	5432.0
23	8738.0
24	13855.0
25	23379.0
26	37584.0
27	46205.0
28	44770.0
29	39266.0
30	38402.0
31	41216.0
32	49470.0
33	63964.0
34	113860.0
35	225781.0
36	101049.0
37	128118.0
38	219367.0
39	538140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.493584512345628	24.218444419628156	28.43150561373066	18.856465454295556
2	33.46459240678859	25.143271111263825	29.777827410353545	11.614309071594036
3	24.73305787253782	24.750868331457795	39.16245028390788	11.353623512096508
4	22.194466158696343	26.72949005391816	39.31799114058522	11.758052646800273
5	19.699742006053263	30.24307553982585	38.31608124089075	11.741101213230136
6	19.07878121483365	37.5177603491079	34.959296515506836	8.44416192055161
7	78.20125818579572	4.791872474823398	15.062093215704127	1.944776123676743
8	79.37932555056366	3.9026667010276808	14.388617341431079	2.3293904069775766
9	73.93837352140261	6.1390532967961216	16.603585571810225	3.318987609991037
10	37.06843282278594	33.16880134466101	21.15286928783935	8.6098965447137
11	32.55746163735959	21.475576477595187	33.1428587790959	12.824103105949321
12	30.342149796840506	20.661735861158594	35.83693408125807	13.159180260742827
13	16.33642868801058	36.5794755936581	33.20195972316476	13.88213599516655
14	12.101777323448815	36.93751735947061	35.81190780939946	15.148797507681119
15	11.252258520815616	24.49568053419923	50.78179897088763	13.470261974097522
16	12.67182654560136	22.58429186245286	45.91593577926484	18.827945812680934
17	12.800794884790385	23.099306193859114	36.1975529231201	27.90234599823041
18	15.332915274329745	25.694421775719935	42.29949632480321	16.67316662514711
19	19.59728891599591	26.862180836289813	34.484370033759696	19.05616021395458
20	22.186047710267932	23.713566587349995	37.107375305311926	16.99301039707015
21	17.115278338530437	27.960244306809496	37.32276159469466	17.601715759965412
22	19.196754029544746	24.59790455082996	32.29706241964534	23.908278999979956
23	14.181420427050135	28.667393974796195	34.75530662909863	22.395878969055044
24	13.1964619609258	24.848052732702808	47.395349236755976	14.560136069615414
25	12.682650265009322	29.62045396626321	41.6761875309607	16.020708237766762
26	14.231931117620613	31.20558481014108	35.58145994221623	18.981024130022078
27	12.968992048288678	33.89456322856088	34.30867071553948	18.827774007610966
28	11.658405706219058	30.217648389470643	40.10783631558301	18.016109588727296
29	12.355132533294391	25.298926504654485	38.92673372791231	23.419207234138813
30	13.92806521720456	31.95643023425621	36.06944360928091	18.046060939258318
31	18.831381914080282	32.48450175098	32.3176217596848	16.366494575254915
32	20.14895499566192	27.34426586261894	33.01200058413723	19.494778557581903
33	20.017409580423383	31.203866759441407	29.82787995407078	18.950843706064433
34	15.30926344303087	28.366276955499625	33.0740794827522	23.250380118717302
35	16.121156935341162	29.15990184203669	34.00091056687083	20.718030655751317
36	22.34279120243505	30.94867896218284	29.902500622793376	16.80602921258873
37	14.552462109823527	33.03479339008628	35.33039546663689	17.082349033453312
38	15.424945523475731	31.057259766402375	34.405568775001214	19.112225935120676
39	16.251900593586516	32.9916703175244	32.601042856774704	18.155386232114378
40	18.040448640306042	28.629825932829945	32.78023554475092	20.549489882113086
41	13.902237188352762	29.321627681232876	33.43240759034799	23.343727540066375
42	15.783388167784832	30.049050347475752	30.93716802249501	23.230393462244404
43	16.153284483425107	28.702442209069588	32.935833669784934	22.208439637720375
44	15.096626034767619	30.325083826557055	31.758453525296865	22.81983661337846
45	14.694373097616777	35.942250589148216	28.523822204659925	20.83955410857508
46	18.721541206014322	33.46648226255823	30.651914337992114	17.160062193435326
47	16.169835038498654	31.14659840278553	32.31298302279567	20.370583535920144
48	16.259288211595123	28.12534897904837	34.46581508620319	21.14954772315331
49	17.791445825566313	27.249028585500223	35.33251439583315	19.62701119310031
50	16.62420218020634	31.360037568041964	32.37483284798401	19.640927403767687
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4590.0
1	5775.5
2	6961.0
3	21910.0
4	36859.0
5	27099.5
6	17340.0
7	18848.0
8	20356.0
9	21969.5
10	23583.0
11	23438.5
12	23294.0
13	22153.5
14	21013.0
15	19558.5
16	18104.0
17	16599.5
18	15095.0
19	14387.0
20	13679.0
21	15077.5
22	16476.0
23	18014.5
24	19553.0
25	22889.5
26	26226.0
27	29209.5
28	32193.0
29	41462.0
30	50731.0
31	63913.0
32	77095.0
33	90560.0
34	104025.0
35	110787.5
36	117550.0
37	113154.0
38	108758.0
39	117975.5
40	127193.0
41	137623.0
42	148053.0
43	145662.0
44	143271.0
45	140786.5
46	138302.0
47	127937.5
48	117573.0
49	107567.5
50	97562.0
51	80211.0
52	62860.0
53	52707.0
54	42554.0
55	35249.0
56	27944.0
57	25212.5
58	22481.0
59	20620.5
60	18760.0
61	16926.5
62	15093.0
63	12730.5
64	10368.0
65	8674.0
66	6980.0
67	6017.5
68	5055.0
69	4223.5
70	3392.0
71	2843.5
72	2295.0
73	1908.5
74	1522.0
75	1064.0
76	606.0
77	539.5
78	473.0
79	342.5
80	212.0
81	137.0
82	62.0
83	49.0
84	36.0
85	29.0
86	22.0
87	12.5
88	3.0
89	6.5
90	10.0
91	6.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1746165.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.49184982174882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.26182926591385	18.15023290068446
2	8.953551768508634	4.206709870341168
3	3.472386948587536	2.4471837815765713
4	1.8443938662571828	1.7331289487307364
5	1.1613281161899376	1.364087284965424
6	0.8036329516944763	1.1327294767809217
7	0.6082569668070187	1.000235692208406
8	0.46806869121013156	0.8796639520136746
9	0.3757948260181255	0.7945304054947228
>10	3.769973192480241	19.697739634018912
>50	0.6972559453657654	11.283790997742047
>100	0.5201646306934059	24.85719546634857
>500	0.05599505931166933	9.026140582978382
>1k	0.0071221785966596955	2.1633013352638413
>5k	0.0	0.0
>10k+	2.455923654020585E-4	1.2633296708521529
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21897	1.25400520569362	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3172	0.18165522731242467	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09810069495150801	0.0
2	0.0	0.0	0.0	0.3657157256043959	0.0
3	0.0	0.0	0.0	0.5218292658483018	0.0
4	0.0	0.0	0.0	0.8193956470322106	0.0
5	0.0	0.0	0.0	1.4558188945489114	0.0
6	0.0	0.0	0.0	2.0386389602357164	0.0
7	0.0	0.0	0.0	2.4069890302462826	0.0
8	0.0	0.0	0.0	3.268476919420559	0.0
9	0.0	0.0	0.0	3.585342736797496	0.0
10	0.0	0.0	0.0	4.123035337439474	0.0
11	0.0	0.0	0.0	4.737066657503729	0.0
12	0.0	0.0	0.0	5.262332024751384	0.0
13	0.0	0.0	0.0	5.467295473222748	0.0
14	0.0	0.0	0.0	5.538880919042588	0.0
15	0.0	0.0	0.0	5.659316273089885	0.0
16	0.0	0.0	0.0	5.929393843078976	0.0
17	0.0	0.0	0.0	6.278788087036449	0.0
18	0.0	0.0	0.0	6.746155145705017	0.0
19	0.0	0.0	0.0	6.971677934215839	0.0
20	0.0	0.0	0.0	7.23327978741986	0.0
21	0.0	0.0	0.0	7.58908808732279	0.0
22	0.0	0.0	0.0	7.954173861003971	0.0
23	0.0	0.0	0.0	8.40224148347951	0.0
24	0.0	0.0	0.0	8.707481824455307	0.0
25	0.0	0.0	0.0	8.977273052661117	0.0
26	0.0	0.0	0.0	9.265848301850054	0.0
27	0.0	0.0	0.0	9.521952392815113	0.0
28	0.0	0.0	0.0	9.829426199700487	0.0
29	0.0	0.0	0.0	10.141137864978395	0.0
30	0.0	0.0	0.0	10.554959010173723	0.0
31	0.0	0.0	0.0	10.942780321447286	0.0
32	0.0	0.0	0.0	11.283469775193065	0.0
33	0.0	0.0	0.0	11.599648372290133	0.0
34	0.0	0.0	0.0	11.933408354880553	0.0
35	0.0	0.0	0.0	12.367731571758682	0.0
36	0.0	0.0	0.0	12.720275575332227	0.0
37	0.0	0.0	0.0	13.0696698192897	0.0
38	0.0	0.0	0.0	13.385275732820208	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGACT	30	2.5284753E-6	44.000004	11
TCGAGTA	30	2.5284753E-6	44.000004	39
AGGTACG	20	7.8576076E-4	44.0	1
GTACCGG	20	7.8576076E-4	44.0	2
CACGGTC	25	4.4437937E-5	44.0	32
ACGTTAG	90	0.0	44.0	1
CTATACG	50	2.7284841E-11	44.0	1
TCGGGTA	25	4.4437937E-5	44.0	5
AAGCGAC	20	7.8576076E-4	44.0	15
TACGGAC	80	0.0	44.0	4
TCGATAG	70	0.0	44.0	1
CTGCGTA	20	7.8576076E-4	44.0	20
TACGAAG	55	1.8189894E-12	44.0	1
GTGTCGA	20	7.8576076E-4	44.0	42
TCGCTAG	25	4.4437937E-5	44.0	14
CATACGT	35	1.4466605E-7	44.0	13
GCGATCG	40	8.3164196E-9	44.0	9
ACGTACT	75	0.0	44.0	39
GCGTATC	35	1.4466605E-7	44.0	9
CCCATAG	35	1.4466605E-7	44.0	25
>>END_MODULE