FastQCFastQC Report
Thu 26 May 2016
SRR1546181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546181_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2334461
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT403941.7303351822968986No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCC62460.2675564080959159No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTT46330.19846122938014388No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31100.13322133031993252No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATG28220.12088443542213813No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG600.044.01
CTATACG207.858153E-444.01
ACTACCG254.4442553E-544.01
ACGAATC1050.044.029
CGAATCC1100.042.030
CGTTTTT228000.041.983331
TCGCTAA750.041.06666611
CTCGTAG650.040.6153831
TCACGAC2350.040.2553225
TACGTTG1150.040.1739161
CGTAAGG3900.039.487182
ACGACGG2400.039.41666427
TAATACG950.039.3684243
CGTACTA950.039.3684228
GATGCCA50400.039.111119
TACGTAG850.038.8235321
TAACGGG4500.038.6222233
CACGACG2450.038.61224426
TCGATAG404.1269777E-738.51
CGAATCA404.1269777E-738.533
Produced by FastQC (version 0.11.5)