FastQCFastQC Report
Thu 26 May 2016
SRR1546180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546180_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2393997
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417321.7431934960653668No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCC59320.24778644250598478No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTT45070.18826255839084177No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32120.1341689233528697No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATG28670.11975787772499297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAA800.044.00000411
CGTTTTT238200.042.3098261
TCACGAC2200.042.025
CTAGACG800.041.2500042
TATACGA800.041.25000426
TCCGATT650.040.61538313
TCGTAAG1700.040.117651
CACGACC1050.039.80952527
CGCTATA501.3496901E-939.60000227
CGTAAGG4200.039.2857132
ATAGCGG3200.039.1875042
CCGATGA6450.038.8837218
CGAATCC850.038.82353230
TAATGCG850.038.8235321
GACCGAT4600.038.739139
ATCCTAG35800.038.7150814
GCGAATC404.1270323E-738.50000432
TAACGGG5200.038.4999963
CCCTACA35100.038.35897427
GTCGTTT1550.038.3225829