##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546180_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2393997
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.778371067298746	31.0	31.0	34.0	30.0	34.0
2	32.178749597430574	33.0	31.0	34.0	30.0	34.0
3	32.23391215611381	33.0	31.0	34.0	30.0	34.0
4	35.942607279791915	37.0	35.0	37.0	35.0	37.0
5	35.844387858464316	37.0	35.0	37.0	35.0	37.0
6	35.91249696637047	37.0	35.0	37.0	35.0	37.0
7	36.10738568176986	37.0	35.0	37.0	35.0	37.0
8	35.98545737525987	37.0	35.0	37.0	35.0	37.0
9	37.712287024586914	39.0	38.0	39.0	35.0	39.0
10	37.422266193316034	39.0	37.0	39.0	34.0	39.0
11	37.33656433153425	39.0	37.0	39.0	34.0	39.0
12	37.035564372052264	39.0	35.0	39.0	33.0	39.0
13	36.87779266222974	39.0	35.0	39.0	33.0	39.0
14	38.03036052259046	40.0	37.0	41.0	33.0	41.0
15	38.14190535744197	40.0	37.0	41.0	33.0	41.0
16	38.22229351164601	40.0	37.0	41.0	33.0	41.0
17	38.10948008706778	40.0	36.0	41.0	33.0	41.0
18	37.96136837264207	39.0	36.0	41.0	33.0	41.0
19	37.76303311992454	39.0	36.0	41.0	33.0	41.0
20	37.50969362116995	39.0	35.0	41.0	33.0	41.0
21	37.447782098306725	39.0	35.0	41.0	33.0	41.0
22	37.36593446023533	39.0	35.0	41.0	33.0	41.0
23	37.29960355004622	39.0	35.0	41.0	33.0	41.0
24	37.240547920486115	39.0	35.0	41.0	33.0	41.0
25	37.24855920872081	39.0	35.0	41.0	33.0	41.0
26	37.17772620433526	39.0	35.0	41.0	33.0	41.0
27	37.10662586461052	39.0	35.0	41.0	33.0	41.0
28	37.03013746466683	39.0	35.0	41.0	33.0	41.0
29	36.95043143328918	39.0	35.0	41.0	32.0	41.0
30	36.77213755906962	39.0	35.0	41.0	32.0	41.0
31	36.562380821696934	39.0	35.0	41.0	31.0	41.0
32	36.279793165989766	39.0	35.0	41.0	30.0	41.0
33	35.881345715972074	39.0	35.0	41.0	29.0	41.0
34	35.466082037696786	39.0	35.0	41.0	25.0	41.0
35	35.168926694561435	39.0	35.0	41.0	23.0	41.0
36	34.993401411948305	38.0	35.0	41.0	22.0	41.0
37	34.9048282015391	38.0	35.0	41.0	21.0	41.0
38	34.840929207513625	38.0	35.0	41.0	21.0	41.0
39	34.74840402891065	38.0	35.0	41.0	20.0	41.0
40	34.64502670638267	38.0	34.0	41.0	20.0	41.0
41	34.523984365895195	38.0	34.0	41.0	18.0	41.0
42	34.45550015309125	38.0	34.0	41.0	18.0	41.0
43	34.36330037172144	38.0	34.0	41.0	18.0	41.0
44	34.23520121370244	38.0	34.0	40.0	18.0	41.0
45	34.187949274790235	38.0	34.0	40.0	18.0	41.0
46	34.11946506198629	38.0	34.0	40.0	18.0	41.0
47	34.05277032510901	38.0	34.0	40.0	18.0	41.0
48	34.01252048352609	38.0	34.0	40.0	18.0	41.0
49	33.90923463980949	37.0	33.0	40.0	18.0	41.0
50	33.74189483111299	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	6.0
12	8.0
13	10.0
14	24.0
15	55.0
16	151.0
17	368.0
18	833.0
19	1681.0
20	3184.0
21	5189.0
22	8300.0
23	12790.0
24	19066.0
25	31506.0
26	49567.0
27	61643.0
28	60491.0
29	54433.0
30	52833.0
31	57104.0
32	67499.0
33	85115.0
34	144802.0
35	231416.0
36	155270.0
37	190803.0
38	328165.0
39	771681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.09389610763923	22.316485776715677	29.09264297323681	19.49697514240828
2	34.72038603222978	24.773005145787568	29.262735082792503	11.24387373919015
3	25.493097944567182	23.832778403648796	39.03893780986359	11.635185841920437
4	23.02225107216091	25.548778883181555	38.843239987351694	12.585730057305836
5	21.142173528204086	28.633285672454896	37.392319205078365	12.832221594262649
6	18.410549386653365	37.57903623103955	34.91746230258434	9.092952079722739
7	76.5246572990693	4.636973229289762	16.670196328566828	2.168173143074114
8	76.74692157091258	4.79474285055495	15.701398122052785	2.7569374564796867
9	70.91725678854233	6.47682515892877	18.095093686416483	4.510824366112405
10	34.65146364009646	28.94468957145727	25.424259094727354	10.979587693718914
11	29.698032203047873	22.4489420830519	34.07268263076353	13.780343083136696
12	29.157012310374657	20.518613849557873	35.245198719964975	15.079175120102489
13	19.417735277028335	28.43270062577355	35.84946012881387	16.300103968384256
14	15.517229136043195	28.97898368293695	37.293196273846625	18.210590907173234
15	14.236191607591822	24.554959759765783	45.75903812744962	15.44981050519278
16	16.854741254897146	22.814230761358516	41.1501768799209	19.180851103823436
17	16.39442321774004	25.295478649304908	36.34728865575019	21.962809477204857
18	18.371576906737978	25.5282274789818	39.83977423530606	16.26042137897416
19	20.01698414826752	27.04999212613884	34.59636749753655	18.336656228057095
20	21.7351984985779	23.9985680850895	38.10054899818171	16.1656844181509
21	18.04902011155402	28.53863225392513	36.58688795349367	16.82545968102717
22	19.441043576913422	23.007213459331822	36.716796219878304	20.834946743876454
23	17.26698070214791	26.900367878489405	35.9495437964208	19.883107622941885
24	16.23631942730087	23.642051347599853	43.59065612864176	16.53097309645751
25	16.721574839066214	29.043520104661784	36.90727264904676	17.327632407225238
26	15.862718290791507	28.49740413208538	36.424565277233015	19.2153122998901
27	15.833144318894302	29.09393787878598	36.086469615459	18.98644818686072
28	14.706492948821573	26.51131141768348	40.207151470950045	18.5750441625449
29	16.520697394357637	23.772043156277974	38.64420047310001	21.063058976264383
30	17.119862723303328	28.12626749323412	37.40455815107538	17.349311632387174
31	19.49718399814202	28.071839689022166	35.14795549033688	17.283020822498944
32	21.382274079708537	25.04293029606971	34.77932512029046	18.79547050393129
33	20.33386006749382	27.748614555490253	33.853885364100286	18.063640012915638
34	17.09956194598406	25.832237885009878	36.197831492687754	20.870368676318307
35	18.55060804169763	27.113275413461253	35.80952691252328	18.526589632317837
36	20.844303480747886	29.9368796201499	31.727483367773644	17.491333531328568
37	16.628007470351886	30.005133673935262	36.83571867466835	16.531140181044503
38	17.114975499133873	28.99285170365711	35.327446107910745	18.564726689298276
39	17.65587007836685	28.838089604957734	34.635172892864944	18.870867423810473
40	18.95127688129935	27.777144248718777	34.22230687841296	19.04927199156891
41	16.21175799301336	27.40525572922606	34.893736291231775	21.489249986528804
42	17.534232499038218	26.976391365569796	32.73124402411532	22.758132111276662
43	17.66125855629727	27.568413828421672	33.77552269280204	20.99480492247902
44	18.129053628722176	27.46085312554694	32.78354149984315	21.626551745887735
45	17.1219512806407	31.030907724612856	31.097073221060846	20.7500677736856
46	18.474542783470486	30.501541981882184	32.363281992416866	18.66063324223046
47	16.778467140936268	28.56812268352884	35.65509898299789	18.998311192537
48	17.69417421993428	26.03637347916476	36.02343695501707	20.24601534588389
49	17.93820125923299	26.653625714652108	35.44966848329384	19.958504542821064
50	16.956746395254463	29.03449753696433	34.39423691842555	19.614519149355658
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4688.0
1	8285.5
2	11883.0
3	41212.0
4	70541.0
5	48707.0
6	26873.0
7	27396.5
8	27920.0
9	28758.5
10	29597.0
11	28655.5
12	27714.0
13	26825.0
14	25936.0
15	24452.5
16	22969.0
17	21439.5
18	19910.0
19	21862.5
20	23815.0
21	22315.5
22	20816.0
23	23041.5
24	25267.0
25	28568.0
26	31869.0
27	43554.0
28	55239.0
29	63559.5
30	71880.0
31	87162.0
32	102444.0
33	115045.0
34	127646.0
35	131865.0
36	136084.0
37	136394.5
38	136705.0
39	142283.0
40	147861.0
41	153664.0
42	159467.0
43	168909.0
44	178351.0
45	172749.5
46	167148.0
47	157346.0
48	147544.0
49	132323.0
50	117102.0
51	104370.0
52	91638.0
53	82719.5
54	73801.0
55	69410.5
56	65020.0
57	61544.5
58	58069.0
59	55204.5
60	52340.0
61	47498.0
62	42656.0
63	37314.5
64	31973.0
65	27599.0
66	23225.0
67	18651.0
68	14077.0
69	11755.5
70	9434.0
71	7784.5
72	6135.0
73	4876.5
74	3618.0
75	2909.0
76	2200.0
77	1734.0
78	1268.0
79	964.0
80	660.0
81	487.0
82	314.0
83	235.0
84	156.0
85	114.5
86	73.0
87	51.0
88	29.0
89	21.5
90	14.0
91	10.5
92	7.0
93	9.5
94	12.0
95	10.0
96	8.0
97	4.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2393997.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.42194870352399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75274607351001	17.97698881388354
2	8.791125095694374	4.1181056207523214
3	3.3230272259247586	2.3349531967807002
4	1.816569755605878	1.7019041452869594
5	1.1322374520913776	1.3259603761546475
6	0.8236833895505431	1.1575362058798555
7	0.6169332265190401	1.0114844869519954
8	0.4959374272697584	0.9292656785335954
9	0.43110931756932075	0.9087678289547879
>10	4.617989604940693	24.245875799832913
>50	0.6787892059444801	10.879368688429798
>100	0.46752425322967356	22.375715888467294
>500	0.04727561621856176	7.272466987756948
>1k	0.004691473365200785	1.747189094073351
>5k	1.804412832769533E-4	0.25070331404763113
>10k+	1.804412832769533E-4	1.7637138742137122
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41732	1.7431934960653668	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCC	5932	0.24778644250598478	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTT	4507	0.18826255839084177	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3212	0.1341689233528697	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATG	2867	0.11975787772499297	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0838764626689173	0.0
2	0.0	0.0	0.0	0.3065584459796733	0.0
3	0.0	0.0	0.0	0.4518802655141172	0.0
4	0.0	0.0	0.0	0.7445706907736309	0.0
5	0.0	0.0	0.0	1.4141621731355554	0.0
6	0.0	0.0	0.0	1.954388414020569	0.0
7	0.0	0.0	0.0	2.3174632215495676	0.0
8	0.0	0.0	0.0	3.110655527137252	0.0
9	0.0	0.0	0.0	3.436595785207751	0.0
10	0.0	0.0	0.0	4.235803135927071	0.0
11	0.0	0.0	0.0	4.8440328037169635	0.0
12	0.0	0.0	0.0	5.488060344269437	0.0
13	0.0	0.0	0.0	5.69858692387668	0.0
14	4.1771146747468774E-5	0.0	0.0	5.792613775205232	0.0
15	4.1771146747468774E-5	0.0	0.0	5.934844529880364	0.0
16	4.1771146747468774E-5	0.0	0.0	6.214669441941656	0.0
17	4.1771146747468774E-5	0.0	0.0	6.512873658571836	0.0
18	4.1771146747468774E-5	0.0	0.0	6.8995491640131545	0.0
19	4.1771146747468774E-5	0.0	0.0	7.154394930319461	0.0
20	4.1771146747468774E-5	0.0	0.0	7.408906527451789	0.0
21	4.1771146747468774E-5	0.0	0.0	7.757069035591941	0.0
22	4.1771146747468774E-5	0.0	0.0	8.13054485866106	0.0
23	4.1771146747468774E-5	0.0	0.0	8.506151010214298	0.0
24	4.1771146747468774E-5	0.0	0.0	8.803895744230257	0.0
25	4.1771146747468774E-5	0.0	0.0	9.064297073053977	0.0
26	4.1771146747468774E-5	0.0	0.0	9.327538839856524	0.0
27	4.1771146747468774E-5	0.0	0.0	9.624030439470058	0.0
28	4.1771146747468774E-5	0.0	0.0	9.899552923416362	0.0
29	4.1771146747468774E-5	0.0	0.0	10.216470613789408	0.0
30	4.1771146747468774E-5	0.0	0.0	10.582845341911456	0.0
31	4.1771146747468774E-5	0.0	0.0	10.894541638941067	0.0
32	4.1771146747468774E-5	0.0	0.0	11.198593816115894	0.0
33	4.1771146747468774E-5	0.0	0.0	11.500390351366356	0.0
34	4.1771146747468774E-5	0.0	0.0	11.823949654072248	0.0
35	4.1771146747468774E-5	0.0	0.0	12.21668197579195	0.0
36	4.1771146747468774E-5	0.0	0.0	12.513841913753442	0.0
37	4.1771146747468774E-5	0.0	0.0	12.836064539763417	0.0
38	4.1771146747468774E-5	0.0	0.0	13.198011526330234	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCTAA	80	0.0	44.000004	11
CGTTTTT	23820	0.0	42.309826	1
TCACGAC	220	0.0	42.0	25
CTAGACG	80	0.0	41.250004	2
TATACGA	80	0.0	41.250004	26
TCCGATT	65	0.0	40.615383	13
TCGTAAG	170	0.0	40.11765	1
CACGACC	105	0.0	39.809525	27
CGCTATA	50	1.3496901E-9	39.600002	27
CGTAAGG	420	0.0	39.285713	2
ATAGCGG	320	0.0	39.187504	2
CCGATGA	645	0.0	38.88372	18
CGAATCC	85	0.0	38.823532	30
TAATGCG	85	0.0	38.823532	1
GACCGAT	460	0.0	38.73913	9
ATCCTAG	3580	0.0	38.71508	14
GCGAATC	40	4.1270323E-7	38.500004	32
TAACGGG	520	0.0	38.499996	3
CCCTACA	3510	0.0	38.358974	27
GTCGTTT	155	0.0	38.322582	9
>>END_MODULE