FastQCFastQC Report
Thu 26 May 2016
SRR1546177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546177_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1107524
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163461.4759048110921298No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34860.3147561587830151No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC30340.2739444021077647No Hit
AACAGAGGGAAGTTTGCTGTTTGAGATTTTTTTTTTCCATTCTTAAAGTT14720.13290908368577115No Hit
TGGTAAGGGAAGACCCAAAGCATTCAACCAGAAGATGTTGATATTGTACC14280.12893625781472906No Hit
GTAAATGGGGGGGCATACTTTCATTATTGTGGTTTGTAGTGTTAAGATGA13500.1218935210433363No Hit
TATGTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT13400.12099060607264492No Hit
TGGCAGAGGGCAGTACTGATAACTCATGTACTATAAATACATATCATTTG12730.11494107576901268No Hit
AGGAGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT12500.1128643713364225No Hit
AGTAAAGGGATTTCCATGATCACACACTAATAATTGTTTTCCTAATTAGC12180.10997504343021008No Hit
AATCGAGGGTGGAAGGTTTTCATTGAGGATAAATTGCCATAATATGATGC11930.10771775600348164No Hit
GTTTACGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT11490.10374493013243957No Hit
GTGAATGGGCATCTACTCACAGTACTGTCTATTTTATTTTTGGTATAATT11310.10211968318519508No Hit
TAGCAGGGGACACTTGCATCTGTTTTTCTTTTCAGCCTGGTTAAAATAGC11270.10175851719691853No Hit
GTTCAAGGGATTTTTAAGATGGTTTAGCTGTTTAAATTTGTTTTGTCTCT11250.10157793420278026No Hit
TTCTGGGATATTTATTTTACTTATTTTACTCTACTTTGTTATTTTTTTGT11230.10139735120864198No Hit
AATTAAGGGTATTTTTTCCACGTATCCTATTATGGATACTGGTTTTGTTA11220.10130705971157285No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC11180.10094589372329629No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGT254.4427354E-544.028
CGTGGTT254.4427354E-544.032
GTGCACG254.4427354E-544.01
CGGGTAT1250.044.06
TGCCCGC207.856359E-444.034
GTCGATG302.5276331E-644.01
AGTTTCG207.856359E-444.043
CGAGTGT207.856359E-444.025
CGAGTAA207.856359E-444.044
CATGCAA700.044.019
GGCACAC207.856359E-444.08
GTTTACG2150.044.01
GACGTTG302.5276331E-644.01
CTATACG302.5276331E-644.01
ATTACCG207.856359E-444.01
TTGTACG502.7284841E-1144.01
TACGGAC254.4427354E-544.035
CCGCTAA207.856359E-444.033
CCTCGAA502.7284841E-1144.025
TAGCCAA351.4460238E-744.042
Produced by FastQC (version 0.11.5)