##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546177_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1107524
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.956624867722958	33.0	31.0	34.0	30.0	34.0
2	32.31298373669555	33.0	31.0	34.0	31.0	34.0
3	32.24076047110491	34.0	31.0	34.0	30.0	34.0
4	35.98403556040321	37.0	35.0	37.0	35.0	37.0
5	35.959922313195925	37.0	35.0	37.0	35.0	37.0
6	35.99710254585905	37.0	35.0	37.0	35.0	37.0
7	36.19790722368093	37.0	35.0	37.0	35.0	37.0
8	36.103344938800426	37.0	35.0	37.0	35.0	37.0
9	37.85294765621332	39.0	38.0	39.0	35.0	39.0
10	37.60133505007566	39.0	37.0	39.0	35.0	39.0
11	37.40975545450934	39.0	37.0	39.0	35.0	39.0
12	36.691111885611505	39.0	35.0	39.0	33.0	39.0
13	36.45309176144264	39.0	35.0	39.0	33.0	39.0
14	37.55258937955295	40.0	35.0	41.0	33.0	41.0
15	37.7976125122345	40.0	35.0	41.0	33.0	41.0
16	37.90917036560833	40.0	35.0	41.0	34.0	41.0
17	37.858129485230116	40.0	35.0	41.0	34.0	41.0
18	37.6666790065046	39.0	36.0	41.0	34.0	41.0
19	37.44909275103745	38.0	36.0	41.0	34.0	41.0
20	37.13014345513054	38.0	35.0	41.0	34.0	41.0
21	37.02109480246026	38.0	35.0	41.0	33.0	41.0
22	36.9809457853735	38.0	35.0	41.0	33.0	41.0
23	36.89479144470007	38.0	35.0	41.0	33.0	41.0
24	36.81961022966545	38.0	35.0	41.0	33.0	41.0
25	36.85259642228972	38.0	35.0	41.0	33.0	41.0
26	36.78602721024556	38.0	35.0	41.0	33.0	41.0
27	36.72557344129789	38.0	35.0	41.0	33.0	41.0
28	36.69860607986825	38.0	35.0	41.0	33.0	41.0
29	36.6361243638964	38.0	35.0	41.0	33.0	41.0
30	36.460374673596235	38.0	35.0	41.0	32.0	41.0
31	36.14134682408688	38.0	35.0	41.0	31.0	41.0
32	35.76919506936193	38.0	35.0	41.0	30.0	41.0
33	35.341894171142116	38.0	35.0	41.0	27.0	41.0
34	34.906366814624334	37.0	35.0	41.0	23.0	41.0
35	34.67523322293693	37.0	35.0	41.0	23.0	41.0
36	34.51267963493342	37.0	35.0	40.0	21.0	41.0
37	34.48151733054995	37.0	34.0	40.0	21.0	41.0
38	34.444831895290754	37.0	34.0	40.0	21.0	41.0
39	34.390296733975966	37.0	34.0	40.0	21.0	41.0
40	34.27148215298269	37.0	34.0	40.0	20.0	41.0
41	34.154922150671226	37.0	34.0	40.0	18.0	41.0
42	34.07291489845818	37.0	34.0	40.0	18.0	41.0
43	33.939477609514555	36.0	34.0	40.0	18.0	41.0
44	33.786116598827654	36.0	34.0	40.0	18.0	41.0
45	33.74637840805256	36.0	34.0	40.0	18.0	41.0
46	33.6770670432424	36.0	34.0	40.0	18.0	41.0
47	33.66483254538954	36.0	33.0	40.0	18.0	41.0
48	33.58707982851839	36.0	34.0	40.0	18.0	41.0
49	33.5357455007747	36.0	34.0	40.0	17.0	41.0
50	33.35588845027286	35.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	8.0
12	4.0
13	4.0
14	9.0
15	10.0
16	44.0
17	131.0
18	304.0
19	651.0
20	1346.0
21	2233.0
22	3479.0
23	5506.0
24	8802.0
25	14933.0
26	24304.0
27	30981.0
28	29455.0
29	25354.0
30	24328.0
31	25790.0
32	31364.0
33	41044.0
34	78466.0
35	179048.0
36	61807.0
37	72642.0
38	125573.0
39	319899.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.89901076635811	24.898873523282568	28.611479299771382	19.59063641058794
2	34.78750799079749	24.58231153455817	29.929103116501317	10.70107735814303
3	23.16491561356684	24.616170845959097	40.641376620280916	11.577536920193152
4	21.298680660644827	27.10848703955851	38.6118946406579	12.980937659138764
5	20.306196524860862	29.22672556080049	39.39165200934698	11.075425904991674
6	19.14125562967484	36.55667958437018	35.336931750463194	8.965133035491782
7	75.82463224273243	6.140995590163283	16.092021482152983	1.9423506849512968
8	77.00645764787039	4.1993672371885395	16.175811991433143	2.618363123507933
9	72.52655472928802	6.669652305503086	17.797898736280207	3.005894228928673
10	42.85433092194842	25.757184494421793	22.067241883697328	9.321242699932462
11	38.60819268927806	18.298565087528576	31.461620696255792	11.631621526937565
12	35.36726969347843	18.818192653161468	33.90969405629133	11.904843597068778
13	16.126603125530462	38.578938244227665	32.95431972580278	12.34013890443909
14	11.089421087037392	38.754916372015416	37.47142274117762	12.684239799769575
15	10.152737096442154	23.167443775484774	54.66933447943341	12.010484648639668
16	11.22359425168213	20.034328827185686	51.76077448434526	16.981302436786923
17	12.28262322080605	21.114395715126715	35.97628584120976	30.626695222857474
18	15.271542648285727	24.461952968965008	43.52330062373366	16.743203759015607
19	20.616167234299212	24.756122666416257	35.88626521863183	18.7414448806527
20	22.109678887319824	21.412809112940216	39.9334009917618	16.544111007978156
21	15.98863771800882	27.267309782903126	39.680584799968216	17.06346769911984
22	19.654833665004098	23.747386061159848	32.46033494533753	24.137445328498526
23	14.361494649325884	28.401370986091496	34.37415351721498	22.86298084736764
24	12.268808621754472	23.993069224684973	49.96785622704339	13.770265926517167
25	12.651463986333479	26.224081825766305	44.337459052806075	16.786995135094138
26	12.156576290897533	32.30078987001636	37.41562259598889	18.127011243097215
27	12.970373553981673	34.66082902040949	36.510089171882505	15.85870825372633
28	10.39814938547607	28.658340586750263	43.558424016093554	17.38508601168011
29	10.797147511024592	24.10259281062984	43.326013702637596	21.774245975707977
30	13.410002853211308	32.079214536208696	38.68322492334252	15.827557687237478
31	19.001213517720608	30.365211047345248	35.12248944492399	15.511085990010148
32	20.207688501558433	25.96422289720132	37.4586013485938	16.369487252646444
33	21.31926712197659	30.020478111535283	31.779898223424503	16.880356543063627
34	14.45738421921331	27.04230337220683	35.4846486396683	23.015663768911555
35	16.259241334725026	26.3969900426537	36.57311263683676	20.770655985784504
36	23.528700055258398	27.50847837157479	33.32370224031263	15.639119332854188
37	15.16120643886724	31.75226902532135	37.88071409739202	15.205810438419393
38	14.91389802839487	31.405910842564133	33.148988193483845	20.531202935557154
39	15.849408229528208	31.76364575395206	34.2275201259747	18.159425890545037
40	19.519938168382808	25.597729710597694	32.27604999981942	22.60628212120008
41	13.581285823151463	28.419158411014116	34.20485696021034	23.794698805624076
42	16.29698318049993	27.948378545295633	33.20578154514033	22.54885672906411
43	16.65679479632044	26.81422705061019	34.87238199804248	21.65659615502689
44	13.94497997334595	29.88567290641106	35.416659142375245	20.752687977867748
45	15.17691715935727	36.557582499340874	28.8399167873563	19.425583553945557
46	19.21466261679205	30.980818474362632	33.25887294541698	16.54564596342833
47	16.46600886301335	30.2965895095727	33.45345112160098	19.783950505812967
48	16.938413975679083	26.9996857855902	36.73220625467258	19.32969398405813
49	19.193082948992526	25.758538866877828	35.812858231514625	19.23551995261502
50	17.27781971316197	30.41559370270983	32.65446166403617	19.652124920092024
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4320.0
1	4705.0
2	5090.0
3	16195.5
4	27301.0
5	19801.5
6	12302.0
7	13458.5
8	14615.0
9	15805.5
10	16996.0
11	17231.0
12	17466.0
13	16278.5
14	15091.0
15	13812.5
16	12534.0
17	11195.0
18	9856.0
19	10482.5
20	11109.0
21	11295.5
22	11482.0
23	11520.5
24	11559.0
25	12482.0
26	13405.0
27	16419.0
28	19433.0
29	24802.0
30	30171.0
31	39443.0
32	48715.0
33	54080.0
34	59445.0
35	59314.0
36	59183.0
37	59309.5
38	59436.0
39	70244.5
40	81053.0
41	96147.0
42	111241.0
43	110839.0
44	110437.0
45	102356.5
46	94276.0
47	83884.0
48	73492.0
49	60162.5
50	46833.0
51	39695.5
52	32558.0
53	27934.0
54	23310.0
55	20105.5
56	16901.0
57	15752.0
58	14603.0
59	13207.0
60	11811.0
61	10794.0
62	9777.0
63	8729.0
64	7681.0
65	6775.0
66	5869.0
67	4709.0
68	3549.0
69	2937.0
70	2325.0
71	1694.5
72	1064.0
73	767.5
74	471.0
75	436.5
76	402.0
77	301.0
78	200.0
79	135.0
80	70.0
81	69.0
82	68.0
83	42.0
84	16.0
85	11.5
86	7.0
87	3.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1107524.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.89612008745292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.8805540590339	17.052819641539752
2	8.999450151461842	3.9410608247490977
3	3.304298274550094	2.1705393547293705
4	1.6906137078737709	1.4807152307639257
5	1.0671361591605075	1.1683070745320874
6	0.7187828461938637	0.9443133310237227
7	0.5285458366499521	0.810117217770744
8	0.41150712485289687	0.7208327538097211
9	0.3413596381764318	0.6727006467468566
>10	3.643338504457247	18.499643245615303
>50	0.7952709891681287	11.944976721224181
>100	0.5283848415242781	23.88205890139485
>500	0.07873970187420615	11.917663440343155
>1k	0.011603745539356695	3.3109863526469594
>5k	0.0	0.0
>10k+	4.144194835484534E-4	1.483265263110261
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16346	1.4759048110921298	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3486	0.3147561587830151	No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC	3034	0.2739444021077647	No Hit
AACAGAGGGAAGTTTGCTGTTTGAGATTTTTTTTTTCCATTCTTAAAGTT	1472	0.13290908368577115	No Hit
TGGTAAGGGAAGACCCAAAGCATTCAACCAGAAGATGTTGATATTGTACC	1428	0.12893625781472906	No Hit
GTAAATGGGGGGGCATACTTTCATTATTGTGGTTTGTAGTGTTAAGATGA	1350	0.1218935210433363	No Hit
TATGTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1340	0.12099060607264492	No Hit
TGGCAGAGGGCAGTACTGATAACTCATGTACTATAAATACATATCATTTG	1273	0.11494107576901268	No Hit
AGGAGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1250	0.1128643713364225	No Hit
AGTAAAGGGATTTCCATGATCACACACTAATAATTGTTTTCCTAATTAGC	1218	0.10997504343021008	No Hit
AATCGAGGGTGGAAGGTTTTCATTGAGGATAAATTGCCATAATATGATGC	1193	0.10771775600348164	No Hit
GTTTACGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT	1149	0.10374493013243957	No Hit
GTGAATGGGCATCTACTCACAGTACTGTCTATTTTATTTTTGGTATAATT	1131	0.10211968318519508	No Hit
TAGCAGGGGACACTTGCATCTGTTTTTCTTTTCAGCCTGGTTAAAATAGC	1127	0.10175851719691853	No Hit
GTTCAAGGGATTTTTAAGATGGTTTAGCTGTTTAAATTTGTTTTGTCTCT	1125	0.10157793420278026	No Hit
TTCTGGGATATTTATTTTACTTATTTTACTCTACTTTGTTATTTTTTTGT	1123	0.10139735120864198	No Hit
AATTAAGGGTATTTTTTCCACGTATCCTATTATGGATACTGGTTTTGTTA	1122	0.10130705971157285	No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	1118	0.10094589372329629	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11376728630711389	0.0
2	0.0	0.0	0.0	0.37642525128123633	0.0
3	0.0	0.0	0.0	0.5641412736879743	0.0
4	0.0	0.0	0.0	0.9151043227957136	0.0
5	0.0	0.0	0.0	1.6486324449853909	0.0
6	0.0	0.0	0.0	2.3038778392161254	0.0
7	0.0	0.0	0.0	2.677142888099942	0.0
8	0.0	0.0	0.0	3.5546859481148942	0.0
9	0.0	0.0	0.0	3.8957169325450285	0.0
10	0.0	0.0	0.0	4.463831032104045	0.0
11	0.0	0.0	0.0	5.385797508677013	0.0
12	0.0	0.0	0.0	5.915266847490438	0.0
13	0.0	0.0	0.0	6.200949144217191	0.0
14	0.0	0.0	0.0	6.26803572653956	0.0
15	0.0	0.0	0.0	6.382886510811504	0.0
16	0.0	0.0	0.0	6.6674853095734266	0.0
17	0.0	0.0	0.0	6.975740480567464	0.0
18	0.0	0.0	0.0	7.440651398976455	0.0
19	0.0	0.0	0.0	7.644168433370293	0.0
20	0.0	0.0	0.0	7.914320592601154	0.0
21	0.0	0.0	0.0	8.212372824426378	0.0
22	0.0	0.0	0.0	8.503292027983141	0.0
23	0.0	0.0	0.0	8.844864761395689	0.0
24	0.0	0.0	0.0	9.074024580957163	0.0
25	0.0	0.0	0.0	9.258490109469411	0.0
26	0.0	0.0	0.0	9.483947977651049	0.0
27	0.0	0.0	0.0	9.68385335216212	0.0
28	0.0	0.0	0.0	9.933870507546564	0.0
29	0.0	0.0	0.0	10.152556513448015	0.0
30	0.0	0.0	0.0	10.474626283493631	0.0
31	0.0	0.0	0.0	10.816560182894456	0.0
32	0.0	0.0	0.0	11.074161824032707	0.0
33	0.0	0.0	0.0	11.311538169827472	0.0
34	9.0291497069138E-5	0.0	0.0	11.56724368952727	0.0
35	9.0291497069138E-5	0.0	0.0	11.942766025837814	0.0
36	9.0291497069138E-5	0.0	0.0	12.239915342692347	0.0
37	9.0291497069138E-5	0.0	0.0	12.511692748870454	0.0
38	9.0291497069138E-5	0.0	0.0	12.761168155272482	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGT	25	4.4427354E-5	44.0	28
CGTGGTT	25	4.4427354E-5	44.0	32
GTGCACG	25	4.4427354E-5	44.0	1
CGGGTAT	125	0.0	44.0	6
TGCCCGC	20	7.856359E-4	44.0	34
GTCGATG	30	2.5276331E-6	44.0	1
AGTTTCG	20	7.856359E-4	44.0	43
CGAGTGT	20	7.856359E-4	44.0	25
CGAGTAA	20	7.856359E-4	44.0	44
CATGCAA	70	0.0	44.0	19
GGCACAC	20	7.856359E-4	44.0	8
GTTTACG	215	0.0	44.0	1
GACGTTG	30	2.5276331E-6	44.0	1
CTATACG	30	2.5276331E-6	44.0	1
ATTACCG	20	7.856359E-4	44.0	1
TTGTACG	50	2.7284841E-11	44.0	1
TACGGAC	25	4.4427354E-5	44.0	35
CCGCTAA	20	7.856359E-4	44.0	33
CCTCGAA	50	2.7284841E-11	44.0	25
TAGCCAA	35	1.4460238E-7	44.0	42
>>END_MODULE