FastQCFastQC Report
Thu 26 May 2016
SRR1546176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546176_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1125653
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166901.4826949335185888No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34860.3096869106198802No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC29100.2585166121353561No Hit
AACAGAGGGAAGTTTGCTGTTTGAGATTTTTTTTTTCCATTCTTAAAGTT14940.13272296169423436No Hit
TGGTAAGGGAAGACCCAAAGCATTCAACCAGAAGATGTTGATATTGTACC14680.13041319127653014No Hit
GTAAATGGGGGGGCATACTTTCATTATTGTGGTTTGTAGTGTTAAGATGA13700.12170713354826043No Hit
TATGTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT13430.11930852580679836No Hit
TGGCAGAGGGCAGTACTGATAACTCATGTACTATAAATACATATCATTTG12780.11353409976253782No Hit
AGTAAAGGGATTTCCATGATCACACACTAATAATTGTTTTCCTAATTAGC12420.11033595610725508No Hit
AGGAGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT12420.11033595610725508No Hit
GTTTACGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT12410.11024711878349722No Hit
AATTAAGGGTATTTTTTCCACGTATCCTATTATGGATACTGGTTTTGTTA11860.10536106597681524No Hit
AATCGAGGGTGGAAGGTTTTCATTGAGGATAAATTGCCATAATATGATGC11750.10438385541547883No Hit
TAGCAGGGGACACTTGCATCTGTTTTTCTTTTCAGCCTGGTTAAAATAGC11590.10296245823535317No Hit
GTGAATGGGCATCTACTCACAGTACTGTCTATTTTATTTTTGGTATAATT11550.10260710894032177No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAT650.044.00000414
AGCCGTA207.856413E-444.043
GATACTA502.7284841E-1144.09
ACGCCCG207.856413E-444.017
GTCGATG408.312782E-944.01
CGTATGG351.446042E-744.02
CGAAAAT408.312782E-944.039
CGACGGT302.5276695E-644.028
TAGGTCG454.802132E-1044.03
GACGTAT254.442782E-544.09
ACCGAGG254.442782E-544.02
TAAACGG351.446042E-744.02
AGGCGAG302.5276695E-644.01
CCGCTAA254.442782E-544.033
TCTAGCG207.856413E-444.01
ACCCTAC207.856413E-444.010
CCATGCG254.442782E-544.01
ATCGTAG254.442782E-544.01
GTAATCG207.856413E-444.017
TGGCACG207.856413E-444.01
Produced by FastQC (version 0.11.5)