##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546176_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1125653
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8939513331373	31.0	31.0	34.0	30.0	34.0
2	32.28830998540403	33.0	31.0	34.0	31.0	34.0
3	32.32659620682395	34.0	31.0	34.0	30.0	34.0
4	36.018022427870754	37.0	35.0	37.0	35.0	37.0
5	35.92594964878164	37.0	35.0	37.0	35.0	37.0
6	35.98771557487076	37.0	35.0	37.0	35.0	37.0
7	36.180441930150764	37.0	35.0	37.0	35.0	37.0
8	36.08884176562404	37.0	35.0	37.0	35.0	37.0
9	37.82836629049982	39.0	38.0	39.0	35.0	39.0
10	37.599168660324274	39.0	37.0	39.0	35.0	39.0
11	37.413330751128456	39.0	37.0	39.0	35.0	39.0
12	36.689847581803626	39.0	35.0	39.0	33.0	39.0
13	36.40182187583562	39.0	35.0	39.0	33.0	39.0
14	37.50675474591193	40.0	35.0	41.0	33.0	41.0
15	37.77387880634618	40.0	35.0	41.0	33.0	41.0
16	37.88286088163937	40.0	35.0	41.0	34.0	41.0
17	37.837387720727435	40.0	35.0	41.0	34.0	41.0
18	37.649636255577875	39.0	36.0	41.0	34.0	41.0
19	37.441541043287764	38.0	36.0	41.0	34.0	41.0
20	37.129500831961536	38.0	35.0	41.0	34.0	41.0
21	37.018587433249856	38.0	35.0	41.0	33.0	41.0
22	36.98169595781293	38.0	35.0	41.0	33.0	41.0
23	36.88385230617251	38.0	35.0	41.0	33.0	41.0
24	36.8110821007895	38.0	35.0	41.0	33.0	41.0
25	36.83077200522719	38.0	35.0	41.0	33.0	41.0
26	36.78623696645414	38.0	35.0	41.0	33.0	41.0
27	36.71650410917041	38.0	35.0	41.0	33.0	41.0
28	36.67491491605317	38.0	35.0	41.0	33.0	41.0
29	36.619196146592245	38.0	35.0	41.0	33.0	41.0
30	36.455645745180796	38.0	35.0	41.0	32.0	41.0
31	36.13744022358578	38.0	35.0	41.0	31.0	41.0
32	35.76962527528466	38.0	35.0	41.0	30.0	41.0
33	35.33119620344813	37.0	35.0	41.0	27.0	41.0
34	34.97183501487581	37.0	35.0	41.0	24.0	41.0
35	34.71533412161652	37.0	35.0	41.0	23.0	41.0
36	34.54571168912622	37.0	35.0	41.0	21.0	41.0
37	34.44106931709861	37.0	34.0	40.0	21.0	41.0
38	34.41274620153813	37.0	34.0	40.0	21.0	41.0
39	34.37137554823733	37.0	34.0	40.0	21.0	41.0
40	34.26349150226579	37.0	34.0	40.0	20.0	41.0
41	34.173988786953	37.0	34.0	40.0	18.0	41.0
42	34.069734634030205	37.0	34.0	40.0	18.0	41.0
43	33.9554400867763	36.0	34.0	40.0	18.0	41.0
44	33.799383113623826	36.0	34.0	40.0	18.0	41.0
45	33.75908828031374	36.0	34.0	40.0	18.0	41.0
46	33.70017225557076	36.0	34.0	40.0	18.0	41.0
47	33.6628046120785	36.0	33.0	40.0	18.0	41.0
48	33.610566488962405	36.0	34.0	40.0	18.0	41.0
49	33.55253972583025	36.0	34.0	40.0	17.0	41.0
50	33.35426992154776	35.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	3.0
13	2.0
14	5.0
15	21.0
16	39.0
17	128.0
18	319.0
19	704.0
20	1408.0
21	2281.0
22	3501.0
23	5341.0
24	8945.0
25	15156.0
26	24399.0
27	30900.0
28	30492.0
29	26202.0
30	24911.0
31	26563.0
32	32040.0
33	42430.0
34	81261.0
35	178767.0
36	63317.0
37	74562.0
38	128096.0
39	323856.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.011432475194397	24.900391150736507	28.655367151333493	19.432809222735603
2	34.74569871887695	24.55330372681457	30.076853168782918	10.624144385525556
3	23.07691624328279	24.68114063570212	40.60016719184331	11.641775929171779
4	21.438844830511712	27.041459490624554	38.54429384543905	12.975401833424687
5	20.365156935574287	29.13908637919501	39.43186754710377	11.063889138126937
6	19.161411198655358	36.548741041866364	35.31256968177583	8.977278077702454
7	75.77175204081541	6.128353942111823	16.116867276149932	1.9830267409228244
8	76.9229060820697	4.240649649581176	16.180830149255588	2.6556141190935394
9	72.45767567802866	6.6977123500759115	17.796958743058475	3.0476532288369507
10	42.851127301219826	25.65168839775668	22.088334504505386	9.40884979651811
11	38.54464919473408	18.42255117696128	31.417941408231485	11.614858220073149
12	35.31976550500021	18.745919035439872	33.958511193058605	11.97580426650131
13	16.224005088601906	38.46816025897857	32.92266799804203	12.385166654377503
14	11.169427878751268	38.64077117904008	37.462610591363415	12.727190350845245
15	10.216114557505731	23.22953876549878	54.52239722187922	12.031949455116276
16	11.328979712220374	20.076702145332533	51.58818925548104	17.006128886966053
17	12.339948456584755	21.175886352188463	35.86718109399611	30.616984097230677
18	15.24626150332296	24.50151156706374	43.456287150658326	16.79593977895497
19	20.609814925203416	24.73844070952594	35.99270823246595	18.659036132804694
20	22.0455149144541	21.4924137367377	39.86450531380452	16.597566035003684
21	16.007153181308983	27.221710420529238	39.70770743737191	17.06342896078987
22	19.603643396321957	23.767981784795136	32.5045107151138	24.1238641037691
23	14.334524049596101	28.30978996191544	34.45733276595896	22.8983532225295
24	12.203050140673902	24.099167327764416	49.849287480244804	13.848495051316881
25	12.692099607960891	26.205322599415627	44.258577021515514	16.84400077110797
26	12.171957077358652	32.286681597259545	37.43089566678186	18.110465658599942
27	13.006139547444906	34.591121775538284	36.47234094343461	15.930397733582197
28	10.456952542213276	28.62649502111219	43.50505884140139	17.411493595273146
29	10.855743288562284	24.15051530089646	43.2015017061208	21.792239704420457
30	13.371882809355991	31.99511750068627	38.75945784358057	15.87354184637717
31	18.901917375958664	30.369660987888807	35.265219388212884	15.46320224793964
32	20.186860426792272	25.864631462804255	37.497701334247765	16.45080677615571
33	21.183615199355398	29.99032561544277	31.79114700533823	17.0349121798636
34	14.511310323874232	26.993043149176522	35.43285541814395	23.06279110880529
35	16.259540018105046	26.423684741212433	36.44462369842216	20.872151542260358
36	23.584177361940135	27.483958200262425	33.26282611071085	15.66903832708659
37	15.154048361262307	31.643410535928922	37.84958597365263	15.352955129156143
38	14.876342887195253	31.374144607618863	33.14538316870297	20.604129336482913
39	15.840139012644217	31.676102671071817	34.27823672126312	18.205521595020848
40	19.479537655032235	25.629034880198425	32.3392732929242	22.552154171845142
41	13.604103573659025	28.399426821587113	34.25682692623748	23.739642678516383
42	16.34846617918666	27.87946196563239	33.22569210938007	22.54637974580088
43	16.673966133435435	26.82416339671284	34.76959595896782	21.732274510883904
44	14.003960367893125	29.848097060106443	35.33504552468656	20.812897047313868
45	15.237466608270934	36.4365395019602	28.80123803694389	19.52475585282498
46	19.23132617245279	30.94044079303302	33.19895207492895	16.629280959585234
47	16.477013786664273	30.23711570084209	33.42308864277002	19.862781869723616
48	16.95593579904287	26.92161794087521	36.72845894782851	19.393987312253422
49	19.224752210494707	25.74630014755879	35.799398215968864	19.22954942597763
50	17.315193936319627	30.321155809117016	32.679164893621746	19.684485360941608
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4336.0
1	4838.5
2	5341.0
3	16605.0
4	27869.0
5	20033.0
6	12197.0
7	13364.5
8	14532.0
9	15840.0
10	17148.0
11	17344.5
12	17541.0
13	16442.5
14	15344.0
15	14041.0
16	12738.0
17	11417.5
18	10097.0
19	10667.5
20	11238.0
21	11473.0
22	11708.0
23	11733.0
24	11758.0
25	12866.5
26	13975.0
27	16807.0
28	19639.0
29	25200.0
30	30761.0
31	40032.0
32	49303.0
33	54732.0
34	60161.0
35	59878.5
36	59596.0
37	59769.0
38	59942.0
39	70930.0
40	81918.0
41	97232.0
42	112546.0
43	112361.0
44	112176.0
45	104330.5
46	96485.0
47	86031.0
48	75577.0
49	61965.0
50	48353.0
51	40931.0
52	33509.0
53	28633.0
54	23757.0
55	20750.5
56	17744.0
57	16236.5
58	14729.0
59	13357.5
60	11986.0
61	10859.5
62	9733.0
63	8744.5
64	7756.0
65	6870.0
66	5984.0
67	4808.5
68	3633.0
69	2963.0
70	2293.0
71	1665.5
72	1038.0
73	791.0
74	544.0
75	460.5
76	377.0
77	265.5
78	154.0
79	105.0
80	56.0
81	57.5
82	59.0
83	37.0
84	15.0
85	9.5
86	4.0
87	2.5
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1125653.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.083771656530292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.78409590412016	17.17766212456243
2	9.094826809532345	4.016961570348045
3	3.3103314232579004	2.1931380977599324
4	1.7355264830258628	1.5330788222001699
5	1.0626383115004332	1.173353091232824
6	0.7231427140255647	0.9581831142974496
7	0.5296305108635203	0.8187367484969029
8	0.4340325915039134	0.766806131381161
9	0.3450182520872219	0.6857373866786474
>10	3.5842041118521086	18.280884989110053
>50	0.7776122762444134	11.801668027176323
>100	0.5289055457941222	23.867255727173458
>500	0.0779227254490577	11.878971523421619
>1k	0.011708596051930949	3.3594479359559837
>5k	0.0	0.0
>10k+	4.0374469144589483E-4	1.4881147102050367
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16690	1.4826949335185888	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3486	0.3096869106198802	No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC	2910	0.2585166121353561	No Hit
AACAGAGGGAAGTTTGCTGTTTGAGATTTTTTTTTTCCATTCTTAAAGTT	1494	0.13272296169423436	No Hit
TGGTAAGGGAAGACCCAAAGCATTCAACCAGAAGATGTTGATATTGTACC	1468	0.13041319127653014	No Hit
GTAAATGGGGGGGCATACTTTCATTATTGTGGTTTGTAGTGTTAAGATGA	1370	0.12170713354826043	No Hit
TATGTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1343	0.11930852580679836	No Hit
TGGCAGAGGGCAGTACTGATAACTCATGTACTATAAATACATATCATTTG	1278	0.11353409976253782	No Hit
AGTAAAGGGATTTCCATGATCACACACTAATAATTGTTTTCCTAATTAGC	1242	0.11033595610725508	No Hit
AGGAGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1242	0.11033595610725508	No Hit
GTTTACGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT	1241	0.11024711878349722	No Hit
AATTAAGGGTATTTTTTCCACGTATCCTATTATGGATACTGGTTTTGTTA	1186	0.10536106597681524	No Hit
AATCGAGGGTGGAAGGTTTTCATTGAGGATAAATTGCCATAATATGATGC	1175	0.10438385541547883	No Hit
TAGCAGGGGACACTTGCATCTGTTTTTCTTTTCAGCCTGGTTAAAATAGC	1159	0.10296245823535317	No Hit
GTGAATGGGCATCTACTCACAGTACTGTCTATTTTATTTTTGGTATAATT	1155	0.10260710894032177	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1003861758463754	0.0
2	0.0	0.0	0.0	0.3685860562713376	0.0
3	0.0	0.0	0.0	0.5529235030688854	0.0
4	0.0	0.0	0.0	0.9075620995102398	0.0
5	0.0	0.0	0.0	1.657615623997804	0.0
6	0.0	0.0	0.0	2.325405786685595	0.0
7	0.0	0.0	0.0	2.704829996455391	0.0
8	0.0	0.0	0.0	3.5883171812272523	0.0
9	0.0	0.0	0.0	3.92874180586735	0.0
10	0.0	0.0	0.0	4.516844889144346	0.0
11	0.0	0.0	0.0	5.44244096537743	0.0
12	0.0	0.0	0.0	5.977952352989775	0.0
13	0.0	0.0	0.0	6.264363884785098	0.0
14	0.0	0.0	0.0	6.336588629000234	0.0
15	0.0	0.0	0.0	6.4677125188668265	0.0
16	0.0	0.0	0.0	6.761675223181567	0.0
17	0.0	0.0	0.0	7.075448650694308	0.0
18	0.0	0.0	0.0	7.569917194730525	0.0
19	0.0	0.0	0.0	7.772377455574675	0.0
20	0.0	0.0	0.0	8.064385738766742	0.0
21	0.0	0.0	0.0	8.370696831083825	0.0
22	0.0	0.0	0.0	8.660928367800734	0.0
23	0.0	0.0	0.0	9.010236724816618	0.0
24	0.0	0.0	0.0	9.260935652461283	0.0
25	0.0	0.0	0.0	9.46597219569441	0.0
26	0.0	0.0	0.0	9.700591567738904	0.0
27	0.0	0.0	0.0	9.903407177878085	0.0
28	0.0	0.0	0.0	10.160058206214526	0.0
29	0.0	0.0	0.0	10.38179616631413	0.0
30	0.0	0.0	0.0	10.722309628277987	0.0
31	0.0	0.0	0.0	11.067620305724766	0.0
32	0.0	0.0	0.0	11.326048080536363	0.0
33	0.0	0.0	0.0	11.566974902567665	0.0
34	0.0	0.0	0.0	11.836507342848995	0.0
35	0.0	0.0	0.0	12.219218533597832	0.0
36	0.0	0.0	0.0	12.525796137886188	0.0
37	0.0	0.0	0.0	12.8002146309742	0.0
38	0.0	0.0	0.0	13.05686565931064	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGAT	65	0.0	44.000004	14
AGCCGTA	20	7.856413E-4	44.0	43
GATACTA	50	2.7284841E-11	44.0	9
ACGCCCG	20	7.856413E-4	44.0	17
GTCGATG	40	8.312782E-9	44.0	1
CGTATGG	35	1.446042E-7	44.0	2
CGAAAAT	40	8.312782E-9	44.0	39
CGACGGT	30	2.5276695E-6	44.0	28
TAGGTCG	45	4.802132E-10	44.0	3
GACGTAT	25	4.442782E-5	44.0	9
ACCGAGG	25	4.442782E-5	44.0	2
TAAACGG	35	1.446042E-7	44.0	2
AGGCGAG	30	2.5276695E-6	44.0	1
CCGCTAA	25	4.442782E-5	44.0	33
TCTAGCG	20	7.856413E-4	44.0	1
ACCCTAC	20	7.856413E-4	44.0	10
CCATGCG	25	4.442782E-5	44.0	1
ATCGTAG	25	4.442782E-5	44.0	1
GTAATCG	20	7.856413E-4	44.0	17
TGGCACG	20	7.856413E-4	44.0	1
>>END_MODULE