FastQCFastQC Report
Thu 26 May 2016
SRR1546175_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546175_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences837749
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126831.5139379456137816No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAA13730.1638915713417742No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9290.11089240333321795No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTT207.8552583E-444.019
CTAACGT351.44546E-744.037
TACGCAT351.44546E-744.027
TCATCGG207.8552583E-444.043
TAGTGCG254.4418033E-544.01
TAAGACG207.8552583E-444.01
TGATGCG302.526891E-644.01
GCGTAAG454.802132E-1044.01
CGTTTTT71600.042.3100551
TTCACGG750.041.0666662
ATCGGGT700.040.8571434
CGGGTAT1250.040.4799966
TTAGCGG1200.040.3333322
GTTCACG603.6379788E-1240.3333321
GGTACGG557.8216544E-1140.02
GCTACGA557.8216544E-1140.010
ATAGGGC3000.039.64
CACGGGA1400.039.2857134
CGACAAT2300.039.21739220
GCGTTAG452.3495886E-839.111111
Produced by FastQC (version 0.11.5)