##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546175_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	837749
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87199387883483	31.0	31.0	34.0	30.0	34.0
2	32.24534198190627	33.0	31.0	34.0	30.0	34.0
3	32.199839092616045	33.0	31.0	34.0	30.0	34.0
4	35.94228939694347	37.0	35.0	37.0	35.0	37.0
5	35.93309093773911	37.0	35.0	37.0	35.0	37.0
6	35.96694236579214	37.0	35.0	37.0	35.0	37.0
7	36.15622817812972	37.0	35.0	37.0	35.0	37.0
8	36.0301343242427	37.0	35.0	37.0	35.0	37.0
9	37.69516645200412	39.0	38.0	39.0	35.0	39.0
10	37.44224821515753	39.0	37.0	39.0	35.0	39.0
11	37.356433728957	39.0	37.0	39.0	34.0	39.0
12	37.15420131805588	39.0	37.0	39.0	34.0	39.0
13	37.08841431025283	39.0	37.0	39.0	33.0	39.0
14	38.298191940545436	40.0	37.0	41.0	33.0	41.0
15	38.36542090769431	40.0	37.0	41.0	33.0	41.0
16	38.39377904360375	40.0	37.0	41.0	34.0	41.0
17	38.29556943666898	40.0	37.0	41.0	34.0	41.0
18	38.105134115349585	40.0	37.0	41.0	34.0	41.0
19	37.96625958371779	40.0	37.0	41.0	34.0	41.0
20	37.757506126536704	40.0	35.0	41.0	34.0	41.0
21	37.668752812596615	40.0	35.0	41.0	33.0	41.0
22	37.59315021563738	39.0	35.0	41.0	33.0	41.0
23	37.528160582704366	39.0	35.0	41.0	33.0	41.0
24	37.44641414075099	39.0	35.0	41.0	33.0	41.0
25	37.42337740779159	39.0	35.0	41.0	33.0	41.0
26	37.39173785943045	39.0	35.0	41.0	33.0	41.0
27	37.323100355834505	39.0	35.0	41.0	33.0	41.0
28	37.2385762322605	39.0	35.0	41.0	33.0	41.0
29	37.15665073906385	39.0	35.0	41.0	33.0	41.0
30	36.99576722860905	39.0	35.0	41.0	32.0	41.0
31	36.78934859964023	39.0	35.0	41.0	31.0	41.0
32	36.54404481533252	39.0	35.0	41.0	31.0	41.0
33	36.21202412655819	39.0	35.0	41.0	30.0	41.0
34	35.783555695082896	39.0	35.0	41.0	26.0	41.0
35	35.57608066377877	39.0	35.0	41.0	24.0	41.0
36	35.465321952040526	39.0	35.0	41.0	24.0	41.0
37	35.43783042414852	39.0	35.0	41.0	23.0	41.0
38	35.33274405579714	39.0	35.0	41.0	23.0	41.0
39	35.244249769322316	39.0	35.0	41.0	23.0	41.0
40	35.14281485265873	39.0	35.0	41.0	23.0	41.0
41	35.016126548644046	39.0	35.0	41.0	22.0	41.0
42	34.95611692762391	39.0	35.0	41.0	22.0	41.0
43	34.84658889476442	38.0	35.0	41.0	21.0	41.0
44	34.72847356427761	38.0	34.0	41.0	21.0	41.0
45	34.67659406337698	38.0	34.0	40.0	20.0	41.0
46	34.622949117217686	38.0	34.0	40.0	20.0	41.0
47	34.562857132625645	38.0	34.0	40.0	20.0	41.0
48	34.45661289956777	38.0	34.0	40.0	20.0	41.0
49	34.39203269714437	38.0	34.0	40.0	20.0	41.0
50	34.27646108798697	38.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	4.0
12	8.0
13	11.0
14	12.0
15	16.0
16	53.0
17	137.0
18	289.0
19	549.0
20	998.0
21	1694.0
22	2508.0
23	3812.0
24	5994.0
25	9911.0
26	15778.0
27	19262.0
28	18949.0
29	17461.0
30	17130.0
31	18745.0
32	21797.0
33	27365.0
34	46469.0
35	73896.0
36	53599.0
37	69941.0
38	123510.0
39	287845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.67231593233773	21.49056578999199	27.349361204847757	18.48775707282253
2	36.87715532934089	23.68119806767898	27.354135904668343	12.087510698311785
3	27.220444309691803	23.73097431330864	36.738450299552724	12.310131077446824
4	24.596866125772756	26.061147193252392	35.73803131964347	13.603955361331376
5	21.72774900357983	29.59669304290426	35.33289803986636	13.342659913649554
6	21.16206644233535	36.381064017981515	33.50920144339176	8.947668096291371
7	77.05780609705293	5.069060064530069	15.375727097257055	2.4974067411599417
8	76.63452895795757	5.15751137870651	15.468714376263057	2.7392452870728583
9	69.23076004865419	8.466378354375834	17.99488868384206	4.307972913127918
10	32.920361588017414	30.305616598766456	25.20504351542049	11.56897829779564
11	27.285022124765295	24.84992521626406	34.17658511081482	13.688467548155833
12	25.765593274357833	23.360218872239777	35.798670007364976	15.075517846037418
13	19.968152752196662	27.84473631123403	36.26300956491741	15.924101371651892
14	16.01995347055025	29.634532538982437	37.794613899867386	16.550900090599928
15	15.91455197201071	26.471652010327674	42.78930801469175	14.824488002969863
16	18.278267118194115	25.143091785248327	40.63723143805603	15.941409658501533
17	18.485489090407746	25.24729960883272	36.538271009574466	19.72894029118507
18	18.98623573409219	25.632021046876808	38.755880341247796	16.625862877783202
19	22.12213920876062	26.48573737479842	35.24540166565403	16.14672175078693
20	22.53753809315201	25.692301632111764	36.19843174984393	15.5717285248923
21	20.252963596494894	27.017758302307733	37.27811074677499	15.451167354422388
22	20.038579574550372	25.070158245488805	36.189598555175834	18.701663624784988
23	18.110555786995867	27.676666877549245	36.11236778557778	18.10040954987711
24	17.09664828009344	25.820502322294626	40.23030764584619	16.85254175176574
25	17.83493623985227	28.10412187898762	37.113741705451154	16.947200175708954
26	16.858629494036997	29.584040088379695	35.84438775814713	17.71294265943618
27	16.725296001547	29.402959597683793	36.64773100296151	17.224013397807695
28	15.911329049631812	27.648376781112244	39.314042750274844	17.126251418981102
29	16.754182935461575	26.45768601335245	38.532066287157605	18.256064764028366
30	18.294739832575154	28.47129629519104	36.49553744618018	16.738426426053625
31	19.381938981723643	28.712538003626385	34.85029525550016	17.055227759149815
32	20.87427141064925	27.936768650275916	33.97509277838589	17.21386716068894
33	20.703814627054165	28.73342731534147	33.918273850520855	16.644484207083508
34	18.35036508548503	27.927816088112312	34.9363592197663	18.78545960663636
35	18.25964578889381	29.124475230647846	34.29141664150002	18.324462338958327
36	19.956454737636214	30.82677508418393	32.34345848219455	16.87331169598531
37	18.64078620207246	31.966376563863403	33.162438868921356	16.230398365142783
38	17.681549008115795	31.525671770422882	33.1506214868654	17.642157734595923
39	17.88220576807612	30.79000989556538	33.5176765355733	17.8101078007852
40	18.753827220324943	29.438292376356163	33.49750342883131	18.310376974487585
41	17.009868110854207	29.196573197938765	33.62904640888858	20.16451228231845
42	17.767612972381944	30.062226275411845	32.077746437178675	20.092414315027533
43	18.19112884646833	29.214000852283917	32.55533578673326	20.03953451451449
44	17.35281092546813	30.819493666957527	32.283774734437166	19.54392067313718
45	16.956988310341163	32.00469352992364	32.0609156203111	18.977402539424098
46	18.449678841753318	31.073746432403976	32.39526397524796	18.08131075059475
47	17.703512627290515	30.7247158755188	33.39007268286802	18.181698814322665
48	18.138249045955295	29.38624814831173	33.704606033549425	18.770896772183555
49	17.788621651592543	29.63524874395553	33.98404534054949	18.592084263902432
50	16.634934807442324	30.517732638296195	33.785179093021895	19.062153461239582
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1472.0
1	2780.0
2	4088.0
3	13237.5
4	22387.0
5	15695.5
6	9004.0
7	9073.0
8	9142.0
9	9384.0
10	9626.0
11	9361.5
12	9097.0
13	9003.5
14	8910.0
15	8136.0
16	7362.0
17	7595.0
18	7828.0
19	7845.0
20	7862.0
21	8715.0
22	9568.0
23	11406.0
24	13244.0
25	16251.0
26	19258.0
27	20951.0
28	22644.0
29	26125.5
30	29607.0
31	33581.0
32	37555.0
33	43969.5
34	50384.0
35	50466.5
36	50549.0
37	51059.0
38	51569.0
39	51086.0
40	50603.0
41	53289.5
42	55976.0
43	54369.0
44	52762.0
45	50898.0
46	49034.0
47	46191.0
48	43348.0
49	39545.5
50	35743.0
51	32375.0
52	29007.0
53	26806.0
54	24605.0
55	23195.5
56	21786.0
57	21018.0
58	20250.0
59	19699.0
60	19148.0
61	17849.5
62	16551.0
63	14673.5
64	12796.0
65	10688.0
66	8580.0
67	7160.5
68	5741.0
69	4887.5
70	4034.0
71	3369.0
72	2704.0
73	2157.0
74	1610.0
75	1360.5
76	1111.0
77	864.5
78	618.0
79	467.5
80	317.0
81	222.5
82	128.0
83	105.0
84	82.0
85	50.0
86	18.0
87	24.0
88	30.0
89	18.0
90	6.0
91	5.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	837749.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.500880162629286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.64856174527921	20.52901150869556
2	7.358397991657208	4.047248427149937
3	3.259115014765409	2.688855943718678
4	2.138896730513562	2.352861706643722
5	1.5628334661776488	2.148964793374904
6	1.2755413204524129	2.1047105397746235
7	1.066793731907439	2.0536436605601383
8	0.9178595437637579	2.0193556255338154
9	0.7491882989146176	1.8543003864925487
>10	6.0634603635392805	31.661456785215325
>50	0.605263767691309	11.572791227867352
>100	0.3510355423611594	14.861434503728974
>500	0.0021803449836096857	0.41972984275207914
>1k	4.3606899672193715E-4	0.1646540211710299
>5k	0.0	0.0
>10k+	4.3606899672193715E-4	1.52098102732132
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12683	1.5139379456137816	No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAA	1373	0.1638915713417742	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	929	0.11089240333321795	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.030319343860750653	0.0
2	0.0	0.0	0.0	0.10337225111578767	0.0
3	0.0	0.0	0.0	0.16210105890905271	0.0
4	0.0	0.0	0.0	0.28767566419058693	0.0
5	0.0	0.0	0.0	0.5636533138207267	0.0
6	0.0	0.0	0.0	0.9102965207956082	0.0
7	0.0	0.0	0.0	1.117279758018213	0.0
8	0.0	0.0	0.0	1.6191007091622909	0.0
9	0.0	0.0	0.0	1.8548515128039544	0.0
10	0.0	0.0	0.0	2.2231002364670087	0.0
11	0.0	0.0	0.0	2.5588810013500463	0.0
12	0.0	0.0	0.0	2.856822270154903	0.0
13	0.0	0.0	0.0	2.9680727759746652	0.0
14	0.0	0.0	0.0	3.013313056774762	0.0
15	0.0	0.0	0.0	3.0743098469828074	0.0
16	0.0	0.0	0.0	3.1964227948944135	0.0
17	0.0	0.0	0.0	3.3319049023036733	0.0
18	0.0	0.0	0.0	3.520028075234945	0.0
19	0.0	0.0	0.0	3.6322335210188252	0.0
20	0.0	0.0	0.0	3.761389151165803	0.0
21	0.0	0.0	0.0	3.9254000900030914	0.0
22	0.0	0.0	0.0	4.110061605564435	0.0
23	0.0	0.0	0.0	4.295200591107838	0.0
24	0.0	0.0	0.0	4.444648695492325	0.0
25	0.0	0.0	0.0	4.572252548197611	0.0
26	0.0	0.0	0.0	4.707376553120326	0.0
27	0.0	0.0	0.0	4.843574865502674	0.0
28	0.0	0.0	0.0	4.985502817669731	0.0
29	0.0	0.0	0.0	5.151423636435257	0.0
30	0.0	0.0	0.0	5.359958651099554	0.0
31	0.0	0.0	0.0	5.544023329183323	0.0
32	0.0	0.0	0.0	5.711854027877085	0.0
33	0.0	0.0	0.0	5.875029394245771	0.0
34	0.0	0.0	0.0	6.058377867356452	0.0
35	0.0	0.0	0.0	6.266316044543174	0.0
36	0.0	0.0	0.0	6.459691387277096	0.0
37	0.0	0.0	0.0	6.647098355235279	0.0
38	0.0	0.0	0.0	6.864466564567669	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACTT	20	7.8552583E-4	44.0	19
CTAACGT	35	1.44546E-7	44.0	37
TACGCAT	35	1.44546E-7	44.0	27
TCATCGG	20	7.8552583E-4	44.0	43
TAGTGCG	25	4.4418033E-5	44.0	1
TAAGACG	20	7.8552583E-4	44.0	1
TGATGCG	30	2.526891E-6	44.0	1
GCGTAAG	45	4.802132E-10	44.0	1
CGTTTTT	7160	0.0	42.310055	1
TTCACGG	75	0.0	41.066666	2
ATCGGGT	70	0.0	40.857143	4
CGGGTAT	125	0.0	40.479996	6
TTAGCGG	120	0.0	40.333332	2
GTTCACG	60	3.6379788E-12	40.333332	1
GGTACGG	55	7.8216544E-11	40.0	2
GCTACGA	55	7.8216544E-11	40.0	10
ATAGGGC	300	0.0	39.6	4
CACGGGA	140	0.0	39.285713	4
CGACAAT	230	0.0	39.217392	20
GCGTTAG	45	2.3495886E-8	39.11111	1
>>END_MODULE