FastQCFastQC Report
Thu 26 May 2016
SRR1546174_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546174_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences853843
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129321.5145641528946188No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAA13680.1602168080080296No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8670.10154091560158016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGG302.5269492E-644.02
TTTACGC207.855343E-444.043
AGCCCGT207.855343E-444.024
GTCGTTA207.855343E-444.036
TTCGGTC351.4454963E-744.029
CGTCGTT207.855343E-444.035
ATGTTCG351.4454963E-744.026
TTACACG408.307325E-944.034
CGTTTTT72850.041.855871
ATAGGGC2050.041.8536574
CGGGTAT1450.040.9655156
TCGTAAG603.6379788E-1240.3333321
AACGGAT501.3478711E-939.64
CGGATGG501.3478711E-939.66
TATCCGG452.3497705E-839.111112
CGTAAGG1300.038.923082
GTTCGGT404.122885E-738.528
ATCGGGT404.122885E-738.54
TAGTACG404.122885E-738.51
TAGTGCG357.2880084E-637.7142871
Produced by FastQC (version 0.11.5)