##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546174_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	853843
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.809828036301756	31.0	31.0	34.0	30.0	34.0
2	32.21505944301236	33.0	31.0	34.0	30.0	34.0
3	32.27702750974125	34.0	31.0	34.0	30.0	34.0
4	35.97196908565158	37.0	35.0	37.0	35.0	37.0
5	35.885784623168426	37.0	35.0	37.0	35.0	37.0
6	35.94827269181805	37.0	35.0	37.0	35.0	37.0
7	36.13089408708627	37.0	35.0	37.0	35.0	37.0
8	36.00449262920701	37.0	35.0	37.0	35.0	37.0
9	37.66160406538439	39.0	38.0	39.0	35.0	39.0
10	37.43986657968737	39.0	37.0	39.0	34.0	39.0
11	37.34689164167183	39.0	37.0	39.0	34.0	39.0
12	37.1311646286261	39.0	37.0	39.0	34.0	39.0
13	37.047030894438436	39.0	37.0	39.0	33.0	39.0
14	38.242872518718315	40.0	37.0	41.0	33.0	41.0
15	38.33346411459718	40.0	37.0	41.0	33.0	41.0
16	38.34319892532936	40.0	37.0	41.0	34.0	41.0
17	38.249935878141535	40.0	37.0	41.0	34.0	41.0
18	38.07940335635474	39.0	37.0	41.0	34.0	41.0
19	37.94636718928421	40.0	37.0	41.0	34.0	41.0
20	37.74149580192143	40.0	35.0	41.0	34.0	41.0
21	37.64297651910246	39.0	35.0	41.0	33.0	41.0
22	37.58310134298694	39.0	35.0	41.0	33.0	41.0
23	37.50113896817096	39.0	35.0	41.0	33.0	41.0
24	37.42174146769371	39.0	35.0	41.0	33.0	41.0
25	37.390514415413605	39.0	35.0	41.0	33.0	41.0
26	37.375762288851696	39.0	35.0	41.0	33.0	41.0
27	37.30179435797916	39.0	35.0	41.0	33.0	41.0
28	37.206987701486106	39.0	35.0	41.0	33.0	41.0
29	37.124436225395065	39.0	35.0	41.0	33.0	41.0
30	36.963509685035774	39.0	35.0	41.0	32.0	41.0
31	36.7697914019322	39.0	35.0	41.0	31.0	41.0
32	36.520990392847395	39.0	35.0	41.0	31.0	41.0
33	36.179826970532055	39.0	35.0	41.0	30.0	41.0
34	35.830693698958704	39.0	35.0	41.0	26.0	41.0
35	35.590331009330754	39.0	35.0	41.0	25.0	41.0
36	35.462813421202725	39.0	35.0	41.0	24.0	41.0
37	35.36592909937775	39.0	35.0	41.0	23.0	41.0
38	35.27342848743856	39.0	35.0	41.0	23.0	41.0
39	35.18961448416161	39.0	35.0	41.0	23.0	41.0
40	35.11220915320498	39.0	35.0	41.0	23.0	41.0
41	35.006504708711084	39.0	35.0	41.0	22.0	41.0
42	34.92854658291981	39.0	35.0	41.0	21.0	41.0
43	34.84243707566848	38.0	35.0	41.0	21.0	41.0
44	34.722071856301454	38.0	34.0	41.0	21.0	41.0
45	34.66525579058445	38.0	34.0	40.0	20.0	41.0
46	34.62699934297055	38.0	34.0	40.0	20.0	41.0
47	34.55092212502767	38.0	34.0	40.0	20.0	41.0
48	34.464066578984664	38.0	34.0	40.0	20.0	41.0
49	34.39336388539814	38.0	34.0	40.0	20.0	41.0
50	34.252006516420465	38.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	6.0
12	2.0
13	9.0
14	12.0
15	26.0
16	63.0
17	141.0
18	274.0
19	570.0
20	1024.0
21	1655.0
22	2672.0
23	4035.0
24	6166.0
25	9991.0
26	15868.0
27	19581.0
28	19490.0
29	18172.0
30	17803.0
31	19202.0
32	22388.0
33	28629.0
34	48459.0
35	74444.0
36	54807.0
37	71562.0
38	125788.0
39	290997.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.70074240814763	21.56977336582955	27.400236343215322	18.329247882807493
2	37.041353035628326	23.553393305326622	27.324109935901564	12.081143723143482
3	27.112478523569322	23.745114734207576	36.79973953056944	12.342667211653664
4	24.725857095508193	25.939663380738615	35.744159055001916	13.59032046875128
5	21.695791849321246	29.487153961559677	35.45300482641422	13.364049362704852
6	21.138546547784546	36.30971970256827	33.566006865430765	8.985726884216419
7	76.99694206077699	5.103162993665112	15.356687353529864	2.543207592028043
8	76.57613870465647	5.187604747008525	15.470525611851361	2.765730936483639
9	69.23169716212465	8.421805882346051	17.99581421877324	4.350682736756055
10	32.92221169465581	30.35183283109424	25.13307481586193	11.592880658388017
11	27.377281303471477	24.81334390514415	34.11294582259268	13.696428968791686
12	25.816924188638897	23.362843051942804	35.7501320500373	15.070100709380998
13	20.046893866905275	27.814129763902734	36.1943589161005	15.944617453091494
14	15.988068064035192	29.558829901984325	37.881554337272775	16.571547696707707
15	15.775851063954379	26.57104409124394	42.7814012646353	14.871703580166376
16	18.35372545069761	25.162119968190872	40.55347411643593	15.93068046467559
17	18.393311182500767	25.167273140378267	36.611063157981036	19.828352519139937
18	19.08161102216684	25.603301777961523	38.64750311239889	16.667584087472758
19	21.988351488505497	26.58954866409867	35.2510941707082	16.171005676687635
20	22.47825419895695	25.80532955121726	36.123619916073565	15.592796333752224
21	20.264146921623766	26.904126402629053	37.3296964430229	15.502030232724284
22	19.93633489997576	25.113399067510066	36.300116063491764	18.650149969022408
23	18.13377869233571	27.639858849929084	36.082043185925286	18.144319271809923
24	17.245090725109886	25.869509968460243	40.06661646227702	16.818782844152846
25	17.825056831290997	28.116644394812628	37.14711018301959	16.911188590876776
26	16.86164786734798	29.528613574158246	35.92170926036754	17.68802929812624
27	16.707169819275908	29.45705475128332	36.50284654204579	17.33292888739499
28	15.834409838811117	27.73203036155359	39.210955644070395	17.2226041555649
29	16.754368191810435	26.58498107965984	38.40670943018799	18.253941298341733
30	18.326319944064657	28.499267429726544	36.389242518823714	16.78517010738508
31	19.214070970892774	28.76688103082183	34.895056819579246	17.123991178706156
32	20.857815781121353	27.966616813629674	33.99032374804267	17.1852436572063
33	20.625220327390398	28.697078971192596	33.948512782794964	16.729187918622042
34	18.34353622387254	27.981256507343854	34.85617379307437	18.819033475709233
35	18.38417601362311	29.0109540044247	34.272342807752715	18.332527174199473
36	19.96233499601215	30.90603307633839	32.29762380203386	16.8340081256156
37	18.6613932537949	31.976019010520666	33.11112230234364	16.25146543334079
38	17.75630882960919	31.43528728349357	33.1750684844872	17.633335402410044
39	17.80046214585117	30.858014880955864	33.51494361375569	17.82657935943727
40	18.784718033643188	29.42379336716469	33.54258335548807	18.24890524370405
41	17.008630392238384	29.207477252843905	33.59692589855512	20.186966456362587
42	17.818615366056758	30.032101920376462	32.04113636816136	20.10814634540542
43	18.157787790026973	29.21122501443474	32.510894860062095	20.120092335476194
44	17.334568533090977	30.851690533271338	32.21774963312928	19.59599130050841
45	16.870314566026774	31.973793777076114	32.11632583507741	19.0395658218197
46	18.409473404361222	31.08756527839427	32.33053383350335	18.172427483741156
47	17.67748871865202	30.74780726667549	33.321816774278176	18.252887240394312
48	18.115039884381552	29.392054511192338	33.67820547805627	18.814700126369836
49	17.79448915081578	29.604505746372578	33.960810125514875	18.640194977296762
50	16.617223541095964	30.471409849351694	33.78782750458808	19.123539104964262
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1438.0
1	2931.5
2	4425.0
3	13678.5
4	22932.0
5	15882.0
6	8832.0
7	9053.5
8	9275.0
9	9476.5
10	9678.0
11	9506.5
12	9335.0
13	9116.5
14	8898.0
15	8209.0
16	7520.0
17	7750.0
18	7980.0
19	7956.5
20	7933.0
21	8758.0
22	9583.0
23	11488.0
24	13393.0
25	16499.5
26	19606.0
27	21340.0
28	23074.0
29	26533.0
30	29992.0
31	34228.5
32	38465.0
33	44940.0
34	51415.0
35	51311.5
36	51208.0
37	51762.5
38	52317.0
39	52463.5
40	52610.0
41	54688.0
42	56766.0
43	55295.5
44	53825.0
45	51879.0
46	49933.0
47	47296.0
48	44659.0
49	40554.0
50	36449.0
51	33076.5
52	29704.0
53	27404.5
54	25105.0
55	23556.5
56	22008.0
57	21355.0
58	20702.0
59	20245.5
60	19789.0
61	18359.5
62	16930.0
63	14839.5
64	12749.0
65	10791.0
66	8833.0
67	7271.0
68	5709.0
69	4884.0
70	4059.0
71	3383.5
72	2708.0
73	2187.0
74	1666.0
75	1434.5
76	1203.0
77	888.0
78	573.0
79	438.5
80	304.0
81	209.5
82	115.0
83	104.0
84	93.0
85	58.0
86	23.0
87	24.0
88	25.0
89	13.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	853843.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.5450277592237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.66460443287794	20.566386017350798
2	7.3459069057945445	4.046864192735676
3	3.3988307076170203	2.808626585706383
4	2.0666429282499834	2.2770294730819662
5	1.5884728539062185	2.187726442781004
6	1.2888104628374624	2.130019198514151
7	1.04559462529728	2.0160653085096083
8	0.9194608601850298	2.026125993385308
9	0.7344564224189967	1.8207560289124254
>10	5.997938949732451	31.444796523319614
>50	0.5935674239175445	11.45535041303381
>100	0.3527242387019671	15.123123321273294
>500	0.002135134616839995	0.4150975118866109
>1k	4.2702692336799887E-4	0.16091056850690955
>5k	0.0	0.0
>10k+	4.2702692336799887E-4	1.5211224210024519
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12932	1.5145641528946188	No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAA	1368	0.1602168080080296	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	867	0.10154091560158016	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.030216327826075754	0.0
2	0.0	0.0	0.0	0.10036974010444544	0.0
3	0.0	0.0	0.0	0.16419880469828763	0.0
4	0.0	0.0	0.0	0.2927938742836798	0.0
5	0.0	0.0	0.0	0.5614615333263844	0.0
6	0.0	0.0	0.0	0.9084808331274017	0.0
7	0.0	0.0	0.0	1.1276077686413075	0.0
8	0.0	0.0	0.0	1.6581502688433354	0.0
9	0.0	0.0	0.0	1.9033944179433455	0.0
10	0.0	0.0	0.0	2.272080464441355	0.0
11	0.0	0.0	0.0	2.618748411593232	0.0
12	0.0	0.0	0.0	2.9171639282631583	0.0
13	0.0	0.0	0.0	3.0313535392337934	0.0
14	1.1711754971347191E-4	0.0	0.0	3.0773807362711882	0.0
15	1.1711754971347191E-4	0.0	0.0	3.137462039274199	0.0
16	1.1711754971347191E-4	0.0	0.0	3.271678751245838	0.0
17	1.1711754971347191E-4	0.0	0.0	3.4194810989842397	0.0
18	1.1711754971347191E-4	0.0	0.0	3.6163557000525857	0.0
19	1.1711754971347191E-4	0.0	0.0	3.731248016321502	0.0
20	1.1711754971347191E-4	0.0	0.0	3.867572844187983	0.0
21	1.1711754971347191E-4	0.0	0.0	4.042780698559337	0.0
22	1.1711754971347191E-4	0.0	0.0	4.232511129095162	0.0
23	1.1711754971347191E-4	0.0	0.0	4.418610915589868	0.0
24	1.1711754971347191E-4	0.0	0.0	4.572737611012798	0.0
25	1.1711754971347191E-4	0.0	0.0	4.711990377622116	0.0
26	1.1711754971347191E-4	0.0	0.0	4.845504384295474	0.0
27	1.1711754971347191E-4	0.0	0.0	4.992252674086454	0.0
28	1.1711754971347191E-4	0.0	0.0	5.142982960567692	0.0
29	1.1711754971347191E-4	0.0	0.0	5.318893520237327	0.0
30	1.1711754971347191E-4	0.0	0.0	5.52747987627702	0.0
31	1.1711754971347191E-4	0.0	0.0	5.716390483964851	0.0
32	1.1711754971347191E-4	0.0	0.0	5.891364103236778	0.0
33	1.1711754971347191E-4	0.0	0.0	6.0687971910526874	0.0
34	1.1711754971347191E-4	0.0	0.0	6.259347444436506	0.0
35	1.1711754971347191E-4	0.0	0.0	6.486321255781215	0.0
36	1.1711754971347191E-4	0.0	0.0	6.681673328703286	0.0
37	1.1711754971347191E-4	0.0	0.0	6.881124515865329	0.0
38	1.1711754971347191E-4	0.0	0.0	7.104116330519779	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGG	30	2.5269492E-6	44.0	2
TTTACGC	20	7.855343E-4	44.0	43
AGCCCGT	20	7.855343E-4	44.0	24
GTCGTTA	20	7.855343E-4	44.0	36
TTCGGTC	35	1.4454963E-7	44.0	29
CGTCGTT	20	7.855343E-4	44.0	35
ATGTTCG	35	1.4454963E-7	44.0	26
TTACACG	40	8.307325E-9	44.0	34
CGTTTTT	7285	0.0	41.85587	1
ATAGGGC	205	0.0	41.853657	4
CGGGTAT	145	0.0	40.965515	6
TCGTAAG	60	3.6379788E-12	40.333332	1
AACGGAT	50	1.3478711E-9	39.6	4
CGGATGG	50	1.3478711E-9	39.6	6
TATCCGG	45	2.3497705E-8	39.11111	2
CGTAAGG	130	0.0	38.92308	2
GTTCGGT	40	4.122885E-7	38.5	28
ATCGGGT	40	4.122885E-7	38.5	4
TAGTACG	40	4.122885E-7	38.5	1
TAGTGCG	35	7.2880084E-6	37.714287	1
>>END_MODULE