FastQCFastQC Report
Thu 26 May 2016
SRR1546173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546173_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3673607
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT501621.3654699590892547No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46200.12576195548407873No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37950.10330446343335038No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGC207.8587444E-444.010
ATCGCGC207.8587444E-444.012
ATACCGG2450.044.02
CCCGCTA207.8587444E-444.025
AACACGC302.529241E-644.017
CATACCG1550.042.5806471
CGGTATG1400.042.4285746
ATTCGAC800.041.2510
TACGCTA750.041.06666619
CGTAAGG2700.040.7407462
CGTTTTT289300.040.6235731
GCGTAAG1200.040.3333321
CGCATTA1100.040.026
CGCAAAT2050.039.70731733
TTACGTC501.3496901E-939.60000242
ACGGGAT13800.039.5362325
AATACGG1800.039.111112
CGGGATC8950.038.8379866
TCACGAC2100.038.761925
TATCGCG2500.038.721
Produced by FastQC (version 0.11.5)