FastQCFastQC Report
Thu 26 May 2016
SRR1546172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546172_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3764802
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510421.3557685105352153No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46510.12353903339405366No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39340.10449420713227417No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC207.8587665E-444.00000432
TCTACGA207.8587665E-444.00000425
CGTACGT351.4472607E-744.042
GTCGGTA502.7284841E-1144.033
ATACCGG1800.041.5555572
AATACGG1500.041.0666662
CGTTTTT295800.040.8911441
CGAAATC2050.040.78048724
CATTCGA603.6379788E-1240.33333211
CATACCG603.6379788E-1240.3333321
CGGTATG1150.040.1739166
ACGGGAT12450.040.112455
TATACGG3950.040.1012652
CTCGCTA557.8216544E-1140.026
TGCGAAG4250.039.341181
TACGGGA15050.039.176084
TATCTCG452.3534085E-839.1111143
TCACGAC2150.038.88372425
ATTCGAC850.038.82353210
GCTACGA2500.038.7210