##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546172_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3764802
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80858541830354	31.0	31.0	34.0	30.0	34.0
2	32.2168533697124	33.0	31.0	34.0	30.0	34.0
3	32.28561448915507	34.0	31.0	34.0	30.0	34.0
4	35.948907273211184	37.0	35.0	37.0	35.0	37.0
5	35.864106797648326	37.0	35.0	37.0	35.0	37.0
6	35.935251574983226	37.0	35.0	37.0	35.0	37.0
7	36.15239473417194	37.0	35.0	37.0	35.0	37.0
8	36.0363108073147	37.0	35.0	37.0	35.0	37.0
9	37.7270329223157	39.0	38.0	39.0	35.0	39.0
10	37.42659056173472	39.0	37.0	39.0	34.0	39.0
11	37.349061916137956	39.0	37.0	39.0	34.0	39.0
12	37.060445941114565	39.0	37.0	39.0	33.0	39.0
13	36.94878376073961	39.0	37.0	39.0	33.0	39.0
14	38.117748024995734	40.0	37.0	41.0	33.0	41.0
15	38.25908347902492	40.0	37.0	41.0	33.0	41.0
16	38.308305722319524	40.0	37.0	41.0	34.0	41.0
17	38.243175338304646	40.0	37.0	41.0	34.0	41.0
18	38.10216818839344	40.0	37.0	41.0	34.0	41.0
19	37.95597006163936	40.0	36.0	41.0	34.0	41.0
20	37.7625699837601	40.0	35.0	41.0	34.0	41.0
21	37.664726325581	40.0	35.0	41.0	33.0	41.0
22	37.59868593354976	39.0	35.0	41.0	33.0	41.0
23	37.528179702411975	39.0	35.0	41.0	33.0	41.0
24	37.47125346830989	39.0	35.0	41.0	33.0	41.0
25	37.43826474805315	39.0	35.0	41.0	33.0	41.0
26	37.40656055750077	39.0	35.0	41.0	33.0	41.0
27	37.34279810731082	39.0	35.0	41.0	33.0	41.0
28	37.25228259016012	39.0	35.0	41.0	33.0	41.0
29	37.177151945839384	39.0	35.0	41.0	33.0	41.0
30	37.02023054598887	39.0	35.0	41.0	32.0	41.0
31	36.8324769801971	39.0	35.0	41.0	31.0	41.0
32	36.603587652152754	39.0	35.0	41.0	31.0	41.0
33	36.30882952144628	39.0	35.0	41.0	30.0	41.0
34	35.98991819490109	39.0	35.0	41.0	28.0	41.0
35	35.774559724522035	39.0	35.0	41.0	26.0	41.0
36	35.6522327070587	39.0	35.0	41.0	25.0	41.0
37	35.56177589153427	39.0	35.0	41.0	24.0	41.0
38	35.49466479246452	39.0	35.0	41.0	24.0	41.0
39	35.41610767312597	39.0	35.0	41.0	24.0	41.0
40	35.323471991355724	39.0	35.0	41.0	23.0	41.0
41	35.24637710030966	39.0	35.0	41.0	23.0	41.0
42	35.17774666503046	39.0	35.0	41.0	23.0	41.0
43	35.097670475100685	39.0	35.0	41.0	23.0	41.0
44	34.982892858641705	39.0	35.0	41.0	22.0	41.0
45	34.93312503552644	38.0	35.0	40.0	23.0	41.0
46	34.89022556830346	38.0	34.0	40.0	23.0	41.0
47	34.834096985711334	38.0	34.0	40.0	23.0	41.0
48	34.76431695478275	38.0	34.0	40.0	22.0	41.0
49	34.666836927944686	38.0	34.0	40.0	22.0	41.0
50	34.51533945211461	38.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	9.0
10	13.0
11	9.0
12	4.0
13	18.0
14	47.0
15	65.0
16	179.0
17	461.0
18	1088.0
19	2264.0
20	4088.0
21	6832.0
22	10901.0
23	16525.0
24	25529.0
25	41689.0
26	65287.0
27	82099.0
28	82370.0
29	76609.0
30	76382.0
31	83935.0
32	100443.0
33	127887.0
34	211669.0
35	345473.0
36	234758.0
37	301514.0
38	532648.0
39	1334006.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.956714855123856	23.868453108556572	29.350547518833658	16.824284517485914
2	35.25316869253682	23.337960402698467	30.18660742317923	11.222263481585486
3	27.001765298679718	23.08769491728914	37.597036975649715	12.313502808381424
4	24.459373959108607	26.226478842712048	36.789796648004334	12.524350550175015
5	21.223161271163796	29.64145259166352	36.086413043766974	13.048973093405708
6	20.0599925308157	38.04040690586118	33.25346193504997	8.646138628273146
7	78.38614089134037	4.802430512945966	14.676309670468726	2.1351189252449396
8	78.12174451671031	4.762401847427833	14.4423531436713	2.6735004921905587
9	71.6266087831445	7.550038488079851	16.85528216357726	3.9680705651983823
10	31.902262057871834	33.82610825217369	24.14219924447554	10.129430445478938
11	28.0579695824641	24.32608673709799	34.06184442103463	13.554099259403282
12	25.65473562753101	23.51472932706687	36.37073078477965	14.459804260622471
13	18.55752307823891	29.813015398950593	37.11828669874272	14.511174824067774
14	14.851006772733333	30.252852606856884	38.2297661337834	16.66637448662639
15	13.816662868326143	27.116220189003297	44.35667533113295	14.710441611537606
16	16.086662724892307	25.576803242242224	41.317206057582844	17.019327975282632
17	16.573487795639718	26.00088928979532	36.57278656354305	20.852836351021914
18	17.366092559449342	26.620390660650944	39.72721540203177	16.286301377867947
19	19.187224188682432	27.23303908147095	36.16163612322773	17.418100606618886
20	20.012181251497424	26.675931430125676	37.513871911457755	15.798015406919141
21	18.45565849146914	27.5254050545022	38.105270874802976	15.91366557922568
22	19.076434829773252	25.438920825052687	36.49798847323179	18.986655871942272
23	16.634420614948674	27.751924271183455	36.75678030345288	18.856874810414997
24	15.603237567340859	26.227700686516847	42.413412445063514	15.755649301078781
25	15.555745029884704	29.279893072729983	38.2780820877167	16.886279809668608
26	15.439908924825263	30.82467550750345	35.83203047597191	17.903385091699377
27	15.215328721138588	30.697311571763937	37.21555609033357	16.87180361676391
28	14.235888102481884	29.189078203847107	39.36709022147779	17.207943472193225
29	14.860808084993579	26.964180320771185	38.4330437563516	19.74196783788364
30	16.792516578561102	30.040650212149274	36.52045977451138	16.646373434778244
31	18.024374190196458	30.931241536739517	34.993553445838586	16.050830827225443
32	18.100872237105698	29.068593779965056	35.08598858585392	17.74454539707533
33	18.588733218905006	29.666712884236674	34.28573401735337	17.458819879504954
34	15.874858757512348	28.85551484513661	36.376043149148344	18.893583248202695
35	18.06166698806471	28.652795020827128	34.74472229880881	18.540815692299358
36	20.026923062620558	30.386246076154865	33.468240826476396	16.118590034748177
37	17.136226553215813	32.58301498989854	34.03703567943281	16.243722777452838
38	16.32269107379352	31.396418722684487	35.26578555791248	17.015104645609515
39	16.723269909015134	30.69805530277555	34.97886475835914	17.599810029850175
40	17.83809613360809	29.703872872995714	34.317634765387396	18.140396228008804
41	15.677026308422063	29.911772252564678	34.550927246638736	19.860274192374526
42	17.130303266944715	28.742919282342072	33.72987477163474	20.396902679078472
43	17.46939148459866	29.433261032054276	33.90704212333079	19.19030536001628
44	16.36375565036355	30.826402025923276	32.895966374858496	19.91387594885468
45	16.020603473967554	33.00330269692802	31.519957756078544	19.456136073025885
46	17.96734595869849	31.57860625870896	32.86959048576791	17.58445729682464
47	16.92888497190556	31.341913864261656	34.107265136386985	17.621936027445802
48	17.687676536508427	28.166899613844233	35.79242679960327	18.352997050044063
49	17.944741848309686	29.30180657575086	34.48959600000212	18.263855575937328
50	16.246830510608525	30.810757112857463	34.6099741765968	18.332438199937208
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8234.0
1	15301.5
2	22369.0
3	56053.5
4	89738.0
5	63388.0
6	37038.0
7	37565.5
8	38093.0
9	39024.5
10	39956.0
11	38502.0
12	37048.0
13	35246.0
14	33444.0
15	32541.5
16	31639.0
17	32821.5
18	34004.0
19	33244.0
20	32484.0
21	40004.5
22	47525.0
23	55416.0
24	63307.0
25	77460.5
26	91614.0
27	105417.0
28	119220.0
29	136536.5
30	153853.0
31	185000.0
32	216147.0
33	224653.5
34	233160.0
35	243903.0
36	254646.0
37	257390.0
38	260134.0
39	260134.0
40	260134.0
41	264549.0
42	268964.0
43	253645.5
44	238327.0
45	229491.0
46	220655.0
47	214366.0
48	208077.0
49	195235.5
50	182394.0
51	152681.0
52	122968.0
53	107318.5
54	91669.0
55	81971.0
56	72273.0
57	65537.0
58	58801.0
59	55262.5
60	51724.0
61	48205.5
62	44687.0
63	39295.0
64	33903.0
65	28482.0
66	23061.0
67	19763.5
68	16466.0
69	14140.5
70	11815.0
71	9273.0
72	6731.0
73	5222.5
74	3714.0
75	3098.0
76	2482.0
77	1867.0
78	1252.0
79	919.5
80	587.0
81	429.5
82	272.0
83	182.5
84	93.0
85	74.5
86	56.0
87	36.0
88	16.0
89	17.5
90	19.0
91	12.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	1.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3764802.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.9627965412769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.47309226239796	14.501436894541495
2	9.370239943680055	3.553719071899016
3	3.5814645141049337	2.0374374870232503
4	1.9071848970078416	1.4466223667422335
5	1.1839188700824108	1.12252063273756
6	0.843405358039991	0.9595994523801089
7	0.6048533537627145	0.8028797759297938
8	0.46471963087716517	0.704990704724879
9	0.3641215913683218	0.621428728806311
>10	3.6968397226114558	15.61265074972563
>50	0.755857214657476	9.92608636237606
>100	0.6114747577473434	24.99627971328782
>500	0.11431904094520778	15.047335857565871
>1k	0.028367007678711607	7.294192197394876
>5k	0.0	0.0
>10k+	1.4183503839355804E-4	1.372820004865134
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51042	1.3557685105352153	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4651	0.12353903339405366	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3934	0.10449420713227417	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06728109472955018	0.0
2	0.0	0.0	0.0	0.26290891260682503	0.0
3	0.0	0.0	0.0	0.38456205664999116	0.0
4	0.0	0.0	0.0	0.5993409480764195	0.0
5	0.0	0.0	0.0	1.0865644461514843	0.0
6	0.0	0.0	0.0	1.530678107374571	0.0
7	0.0	0.0	0.0	1.8208925728364997	0.0
8	0.0	0.0	0.0	2.45285143813672	0.0
9	0.0	0.0	0.0	2.6902078781301113	0.0
10	0.0	0.0	0.0	3.1300185242145537	0.0
11	0.0	0.0	0.0	3.645689733483992	0.0
12	0.0	0.0	0.0	4.0670399133872115	0.0
13	0.0	0.0	0.0	4.229545139425659	0.0
14	0.0	0.0	0.0	4.289707665901155	0.0
15	0.0	0.0	0.0	4.3963533806027515	0.0
16	0.0	0.0	0.0	4.6310005147681075	0.0
17	0.0	0.0	0.0	4.906260674532153	0.0
18	0.0	0.0	0.0	5.264181223873128	0.0
19	0.0	0.0	0.0	5.456887241347619	0.0
20	0.0	0.0	0.0	5.6840970653967995	0.0
21	0.0	0.0	0.0	5.987167452630975	0.0
22	0.0	0.0	0.0	6.306626483942582	0.0
23	0.0	0.0	0.0	6.642181979291341	0.0
24	0.0	0.0	0.0	6.907986130479106	0.0
25	0.0	0.0	0.0	7.149725271076673	0.0
26	0.0	0.0	0.0	7.39451902118624	0.0
27	0.0	0.0	0.0	7.644173584693166	0.0
28	0.0	0.0	0.0	7.90166388564392	0.0
29	0.0	0.0	0.0	8.197881322842477	0.0
30	2.656182184348606E-5	0.0	0.0	8.543424063204386	0.0
31	2.656182184348606E-5	0.0	0.0	8.865937704027994	0.0
32	2.656182184348606E-5	0.0	0.0	9.164572267014307	0.0
33	2.656182184348606E-5	0.0	0.0	9.447933782440616	0.0
34	2.656182184348606E-5	0.0	0.0	9.774192640144156	0.0
35	2.656182184348606E-5	0.0	0.0	10.143216031015708	0.0
36	2.656182184348606E-5	0.0	0.0	10.447800442094962	0.0
37	2.656182184348606E-5	0.0	0.0	10.763886122032448	0.0
38	2.656182184348606E-5	0.0	0.0	11.095218287708091	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGC	20	7.8587665E-4	44.000004	32
TCTACGA	20	7.8587665E-4	44.000004	25
CGTACGT	35	1.4472607E-7	44.0	42
GTCGGTA	50	2.7284841E-11	44.0	33
ATACCGG	180	0.0	41.555557	2
AATACGG	150	0.0	41.066666	2
CGTTTTT	29580	0.0	40.891144	1
CGAAATC	205	0.0	40.780487	24
CATTCGA	60	3.6379788E-12	40.333332	11
CATACCG	60	3.6379788E-12	40.333332	1
CGGTATG	115	0.0	40.173916	6
ACGGGAT	1245	0.0	40.11245	5
TATACGG	395	0.0	40.101265	2
CTCGCTA	55	7.8216544E-11	40.0	26
TGCGAAG	425	0.0	39.34118	1
TACGGGA	1505	0.0	39.17608	4
TATCTCG	45	2.3534085E-8	39.11111	43
TCACGAC	215	0.0	38.883724	25
ATTCGAC	85	0.0	38.823532	10
GCTACGA	250	0.0	38.72	10
>>END_MODULE