FastQCFastQC Report
Thu 26 May 2016
SRR1546171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546171_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3272612
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT516731.578952836449906No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCC88880.2715873436875499No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTT67150.205187782725236No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATG41970.128246183782251No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG800.044.01
TAACGCG408.3200575E-944.01
CGTTTTT293300.042.3798181
TATCGAT800.041.2511
GCGTAAG1600.041.251
CGCACTA1300.040.61538343
CCCTACA14500.040.20689827
TACACCG557.8216544E-1140.019
CGACGGT1800.039.1111128
AGGGCGA15150.039.0627066
AACGGGA13150.038.980994
TAACGGG7050.038.6950343
AAGGGCG6450.038.5426375
ATCGTAC800.038.544
GCCCTAC14400.038.526
ACGGGAC7000.038.3428575
TCGACCA1150.038.26086835
CGGTCTA1850.038.05405431
GGACTAA23350.037.9700248
GGGACTA27950.037.939187