##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546171_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3272612
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84465833407688	31.0	31.0	34.0	30.0	34.0
2	32.21038393796759	33.0	31.0	34.0	30.0	34.0
3	32.1495175718967	33.0	31.0	34.0	30.0	34.0
4	35.89884929835862	37.0	35.0	37.0	35.0	37.0
5	35.88366326347273	37.0	35.0	37.0	35.0	37.0
6	35.92707018124972	37.0	35.0	37.0	35.0	37.0
7	36.12549578135141	37.0	35.0	37.0	35.0	37.0
8	35.98335274698009	37.0	35.0	37.0	35.0	37.0
9	37.67147801205887	39.0	38.0	39.0	35.0	39.0
10	37.38483480473701	39.0	37.0	39.0	34.0	39.0
11	37.31014064606498	39.0	37.0	39.0	34.0	39.0
12	37.09840457713899	39.0	37.0	39.0	33.0	39.0
13	36.99561634559795	39.0	37.0	39.0	33.0	39.0
14	38.21856975406801	40.0	37.0	41.0	33.0	41.0
15	38.30906016356354	40.0	37.0	41.0	33.0	41.0
16	38.36581085689352	40.0	37.0	41.0	34.0	41.0
17	38.23579208289892	40.0	37.0	41.0	34.0	41.0
18	38.06126635238152	39.0	37.0	41.0	34.0	41.0
19	37.884981476569784	39.0	36.0	41.0	34.0	41.0
20	37.64418513407639	39.0	35.0	41.0	33.0	41.0
21	37.57083699503638	39.0	35.0	41.0	33.0	41.0
22	37.47760626679851	39.0	35.0	41.0	33.0	41.0
23	37.40713595134407	39.0	35.0	41.0	33.0	41.0
24	37.33570371311967	39.0	35.0	41.0	33.0	41.0
25	37.33936103638317	39.0	35.0	41.0	33.0	41.0
26	37.280021279638405	39.0	35.0	41.0	33.0	41.0
27	37.20785873791333	39.0	35.0	41.0	33.0	41.0
28	37.12413784463297	39.0	35.0	41.0	33.0	41.0
29	37.03088572675282	39.0	35.0	41.0	32.0	41.0
30	36.86774875848405	39.0	35.0	41.0	32.0	41.0
31	36.663286695764725	39.0	35.0	41.0	31.0	41.0
32	36.369481930641335	39.0	35.0	41.0	30.0	41.0
33	36.03096945192403	39.0	35.0	41.0	29.0	41.0
34	35.59142055336838	39.0	35.0	41.0	25.0	41.0
35	35.356403386652616	39.0	35.0	41.0	23.0	41.0
36	35.233132739230925	39.0	35.0	41.0	23.0	41.0
37	35.18421982196484	39.0	35.0	41.0	23.0	41.0
38	35.09335814939259	39.0	35.0	41.0	22.0	41.0
39	34.971483328912804	39.0	35.0	41.0	21.0	41.0
40	34.83754536131995	39.0	35.0	41.0	21.0	41.0
41	34.722255494999104	38.0	34.0	41.0	20.0	41.0
42	34.670720818722174	38.0	34.0	41.0	19.0	41.0
43	34.53784561078429	38.0	34.0	41.0	18.0	41.0
44	34.40847647078236	38.0	34.0	40.0	18.0	41.0
45	34.38777068592305	38.0	34.0	40.0	18.0	41.0
46	34.30855170120992	38.0	34.0	40.0	18.0	41.0
47	34.24978671470984	38.0	34.0	40.0	18.0	41.0
48	34.17119414094919	38.0	34.0	40.0	18.0	41.0
49	34.10988256475256	38.0	34.0	40.0	18.0	41.0
50	33.97314102618948	38.0	34.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	7.0
11	14.0
12	20.0
13	22.0
14	43.0
15	86.0
16	202.0
17	464.0
18	1109.0
19	2309.0
20	4364.0
21	7294.0
22	11534.0
23	17779.0
24	26887.0
25	42893.0
26	65938.0
27	81194.0
28	78968.0
29	71255.0
30	69907.0
31	75035.0
32	87549.0
33	112155.0
34	181041.0
35	301257.0
36	204536.0
37	258955.0
38	454569.0
39	1115217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.52909296916347	22.218399248062404	26.39756866991871	18.85493911285542
2	35.811058567285095	24.650004339041722	27.88680723532151	11.652129858351676
3	26.38821834057933	24.710170347111116	36.84824232142399	12.053368990885568
4	23.164829805672042	25.65391192111989	38.491333528080936	12.689924745127135
5	21.911518994613477	28.488803438965572	36.51844459410404	13.081232972316915
6	19.750615104998698	37.81080678063883	33.657763278995496	8.780814835366979
7	75.15486101010447	5.351169035620477	16.94686079498578	2.547109159289277
8	74.72725150430298	5.880134889195541	16.40121102043261	2.991402586068865
9	68.15711731179866	7.860999104079555	19.127993174870713	4.853890409251081
10	31.86140000708914	30.673235935087934	26.198889449772842	11.266474608050084
11	27.280196980271416	24.501713004780278	35.163166302635325	13.054923712312977
12	25.31708616847949	23.35247808172799	36.1341643922347	15.196271357557817
13	18.896740585196167	28.675473902803024	37.02666249466787	15.401123017332944
14	14.405679622271139	31.728417545373546	37.745812824740604	16.120090007614714
15	13.899172893089679	25.849443808187466	45.5467987039099	14.70458459481295
16	16.241827628817592	25.423728813559322	41.90991782710569	16.4245257305174
17	15.723556596382341	25.341959266787505	38.15524113460441	20.779243002225744
18	17.571316123023443	26.236412993657666	39.51611128969765	16.676159593621243
19	19.790766519220732	26.104225004369596	37.0395573932993	17.06545108311037
20	20.83888954755406	25.459021723320703	38.02934781147292	15.672740917652323
21	18.01209553714281	27.621300661367737	38.7192248882544	15.647378913235055
22	18.120021560759415	25.605418546408803	36.6266456274071	19.64791426542468
23	16.70647177239465	27.360713705138277	37.745232248735874	18.1875822737312
24	14.99138914115086	26.44795655580313	42.735771915521916	15.824882387524095
25	15.63038942593867	28.93942208853356	38.69630741438338	16.733881071144392
26	15.797442532142522	29.71552998033375	36.742210808980715	17.74481667854301
27	15.66513231632714	30.62718709092309	36.92927850903193	16.77840208371784
28	14.478923868762934	28.77545520214434	40.13020791954561	16.615413009547115
29	15.173537223477759	26.250591270825872	40.14887802159254	18.42699348410383
30	16.24992513625202	30.332865613155484	36.70474837835955	16.712460872232946
31	18.458955720995952	29.477860497975318	34.75104900917066	17.312134771858076
32	19.16658620086952	27.973618626344948	36.02773564357767	16.832059529207864
33	19.343172976203718	27.88289598644752	34.99449369494459	17.779437342404172
34	16.17249463119979	28.112651301162494	35.622463035642475	20.092391031995238
35	17.392407043670318	27.41006266554055	36.07078993782336	19.126740352965765
36	19.828595629423837	29.98843125918991	32.93457947352146	17.2483936378648
37	16.67557901761651	31.010764490260378	34.8036369725467	17.51001951957641
38	16.68022362565437	30.80340718667535	34.37462186168113	18.14174732598915
39	17.545801335447038	29.86803812978746	34.13169663864827	18.45446389611723
40	17.967941204151302	28.484556067141476	34.34324020079374	19.20426252791348
41	15.916277273321738	29.427228159036268	33.19519698638274	21.46129758125925
42	17.96127985841279	29.087346743213065	32.266336492074224	20.68503690629992
43	17.994678257000828	29.41824450927883	33.115505290575236	19.471571943145108
44	16.364665288766282	30.432571902810356	33.380125722205996	19.82263708621737
45	16.49718940100446	31.714086485046195	32.32253013800597	19.466193975943373
46	18.633709098420468	31.48674514424564	31.879611759658644	17.999933997675253
47	16.713072004869506	30.114355138953226	34.6132080429944	18.559364813182864
48	16.968005984210777	29.589208864356664	34.19024314523078	19.25254200620177
49	17.460303879592203	28.523546329354044	34.66445151457001	19.351698276483738
50	16.0375565450472	30.248712649101083	34.157883672124896	19.55584713372682
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5362.0
1	10554.0
2	15746.0
3	52709.0
4	89672.0
5	63655.0
6	37638.0
7	38331.0
8	39024.0
9	39604.0
10	40184.0
11	39357.5
12	38531.0
13	36736.5
14	34942.0
15	33482.5
16	32023.0
17	32802.5
18	33582.0
19	32382.5
20	31183.0
21	36415.5
22	41648.0
23	46207.5
24	50767.0
25	61567.5
26	72368.0
27	80933.0
28	89498.0
29	115187.0
30	140876.0
31	153561.5
32	166247.0
33	180487.0
34	194727.0
35	198654.0
36	202581.0
37	203421.5
38	204262.0
39	210515.0
40	216768.0
41	216959.0
42	217150.0
43	208879.5
44	200609.0
45	186141.5
46	171674.0
47	176597.5
48	181521.0
49	172159.0
50	162797.0
51	148057.0
52	133317.0
53	114446.0
54	95575.0
55	82369.0
56	69163.0
57	64822.0
58	60481.0
59	57395.0
60	54309.0
61	49756.0
62	45203.0
63	39843.5
64	34484.0
65	28981.5
66	23479.0
67	19389.0
68	15299.0
69	13575.5
70	11852.0
71	9837.5
72	7823.0
73	6435.5
74	5048.0
75	3872.0
76	2696.0
77	1971.5
78	1247.0
79	937.0
80	627.0
81	469.0
82	311.0
83	234.0
84	157.0
85	105.0
86	53.0
87	43.5
88	34.0
89	29.5
90	25.0
91	34.0
92	43.0
93	24.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3272612.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.31232796096517
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.53057928327195	15.95138881366397
2	8.390881778827103	3.4087668514644585
3	3.040301188654552	1.852667845321905
4	1.6190368449615051	1.3154562950297763
5	1.012499069706549	1.0283106582025778
6	0.7283427549174437	0.8876602143525597
7	0.5309703523647964	0.7549670754348089
8	0.4220357523745657	0.6858022890787893
9	0.35686332974005225	0.6523852491829798
>10	3.8566608306865033	18.36135837946754
>50	0.8120056893843476	11.290497789658636
>100	0.5983918968247687	25.827065545495675
>500	0.0846783227131614	11.570465192474174
>1k	0.01629600814803646	4.33199102798985
>5k	3.0459828314086834E-4	0.4826866239366838
>10k+	1.5229914157043417E-4	1.5985301492456745
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51673	1.578952836449906	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCC	8888	0.2715873436875499	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTT	6715	0.205187782725236	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATG	4197	0.128246183782251	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.054971380658629866	0.0
2	0.0	0.0	0.0	0.23326932737519754	0.0
3	0.0	0.0	0.0	0.3377424515952395	0.0
4	0.0	0.0	0.0	0.5716840248706537	0.0
5	0.0	0.0	0.0	1.180799923730647	0.0
6	0.0	0.0	0.0	1.5802056583548554	0.0
7	0.0	0.0	0.0	1.8773077896188122	0.0
8	0.0	0.0	0.0	2.4945823091768897	0.0
9	0.0	0.0	0.0	2.717309598571416	0.0
10	0.0	0.0	0.0	3.3730243609691586	0.0
11	0.0	0.0	0.0	3.771696736429494	0.0
12	0.0	0.0	0.0	4.247371824096471	0.0
13	0.0	0.0	0.0	4.395693714989739	0.0
14	0.0	0.0	0.0	4.456440299063867	0.0
15	3.0556631828032165E-5	0.0	0.0	4.5764667488843775	0.0
16	3.0556631828032165E-5	0.0	0.0	4.770562474256038	0.0
17	3.0556631828032165E-5	0.0	0.0	4.98341997157011	0.0
18	3.0556631828032165E-5	0.0	0.0	5.273921870359212	0.0
19	6.111326365606433E-5	0.0	0.0	5.458667266391494	0.0
20	6.111326365606433E-5	0.0	0.0	5.633603983606978	0.0
21	6.111326365606433E-5	0.0	0.0	5.867301103827768	0.0
22	6.111326365606433E-5	0.0	0.0	6.12364068823313	0.0
23	6.111326365606433E-5	0.0	0.0	6.389452828505182	0.0
24	6.111326365606433E-5	0.0	0.0	6.599224106004622	0.0
25	6.111326365606433E-5	0.0	0.0	6.787116835115192	0.0
26	6.111326365606433E-5	0.0	0.0	6.987996132752676	0.0
27	6.111326365606433E-5	0.0	0.0	7.186614239634885	0.0
28	6.111326365606433E-5	0.0	0.0	7.38297115576182	0.0
29	6.111326365606433E-5	0.0	0.0	7.609701363925819	0.0
30	6.111326365606433E-5	0.0	0.0	7.885963872283057	0.0
31	9.16698954840965E-5	0.0	0.0	8.146123035666923	0.0
32	9.16698954840965E-5	0.0	0.0	8.385992595516976	0.0
33	9.16698954840965E-5	0.0	0.0	8.614464531695171	0.0
34	1.2222652731212866E-4	0.0	0.0	8.867198433544827	0.0
35	1.2222652731212866E-4	0.0	0.0	9.154766895678437	0.0
36	1.2222652731212866E-4	0.0	0.0	9.39943384672549	0.0
37	1.5278315914016082E-4	0.0	0.0	9.640006209107588	0.0
38	1.5278315914016082E-4	0.0	0.0	9.886048208586903	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	80	0.0	44.0	1
TAACGCG	40	8.3200575E-9	44.0	1
CGTTTTT	29330	0.0	42.379818	1
TATCGAT	80	0.0	41.25	11
GCGTAAG	160	0.0	41.25	1
CGCACTA	130	0.0	40.615383	43
CCCTACA	1450	0.0	40.206898	27
TACACCG	55	7.8216544E-11	40.0	19
CGACGGT	180	0.0	39.11111	28
AGGGCGA	1515	0.0	39.062706	6
AACGGGA	1315	0.0	38.98099	4
TAACGGG	705	0.0	38.695034	3
AAGGGCG	645	0.0	38.542637	5
ATCGTAC	80	0.0	38.5	44
GCCCTAC	1440	0.0	38.5	26
ACGGGAC	700	0.0	38.342857	5
TCGACCA	115	0.0	38.260868	35
CGGTCTA	185	0.0	38.054054	31
GGACTAA	2335	0.0	37.970024	8
GGGACTA	2795	0.0	37.93918	7
>>END_MODULE