FastQCFastQC Report
Thu 26 May 2016
SRR1546170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546170_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3343110
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT528201.5799659598397897No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCC87060.2604161992874898No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTT62960.1883276350464089No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATG41580.12437520751635454No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34370.10280846277867016No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC254.4446693E-544.026
CGCCGAT207.858642E-444.030
TATCGTG254.4446693E-544.01
CGTTTTT302750.042.2995871
ATCGTAC557.8216544E-1140.044
GCGTAAG1600.039.8751
CGCACTA1450.039.44827743
CGGTCTA2350.039.31914531
TGCGTTG1800.039.111111
CCCTACA14350.038.78745727
AACGTGC2050.038.63414811
GGCGATT8600.038.6279078
TAACGCG404.127687E-738.51
CGCAATA800.038.530
ACGGGAT14600.038.4246565
TAACGGG6650.038.375943
TTATCGT750.038.1333340
TACGGGA14800.037.9054034
AAGGGAT46900.037.8550155
AGGGCGA13600.037.8529436