##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546170_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3343110 Sequences flagged as poor quality 0 Sequence length 50 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78496011199153 31.0 31.0 34.0 30.0 34.0 2 32.18013645976352 33.0 31.0 34.0 30.0 34.0 3 32.23548432447631 33.0 31.0 34.0 30.0 34.0 4 35.931743496325275 37.0 35.0 37.0 35.0 37.0 5 35.847815955801636 37.0 35.0 37.0 35.0 37.0 6 35.91728570103885 37.0 35.0 37.0 35.0 37.0 7 36.1066776145565 37.0 35.0 37.0 35.0 37.0 8 35.96585275387289 37.0 35.0 37.0 35.0 37.0 9 37.64423635477146 39.0 38.0 39.0 35.0 39.0 10 37.378927705041114 39.0 37.0 39.0 34.0 39.0 11 37.305550819446566 39.0 37.0 39.0 34.0 39.0 12 37.08406723081203 39.0 37.0 39.0 33.0 39.0 13 36.96312475509331 39.0 37.0 39.0 33.0 39.0 14 38.16886043235191 40.0 37.0 41.0 33.0 41.0 15 38.284031336091246 40.0 37.0 41.0 33.0 41.0 16 38.32462706880719 40.0 37.0 41.0 34.0 41.0 17 38.206165815662665 40.0 37.0 41.0 33.0 41.0 18 38.04892540179653 39.0 37.0 41.0 34.0 41.0 19 37.870985399822324 39.0 36.0 41.0 34.0 41.0 20 37.62939957105809 39.0 35.0 41.0 33.0 41.0 21 37.55473585972343 39.0 35.0 41.0 33.0 41.0 22 37.46853349127011 39.0 35.0 41.0 33.0 41.0 23 37.379964464226425 39.0 35.0 41.0 33.0 41.0 24 37.31585619378363 39.0 35.0 41.0 33.0 41.0 25 37.309696360574435 39.0 35.0 41.0 33.0 41.0 26 37.27156749254437 39.0 35.0 41.0 33.0 41.0 27 37.19143641698897 39.0 35.0 41.0 33.0 41.0 28 37.09656846469306 39.0 35.0 41.0 33.0 41.0 29 37.004788953997924 39.0 35.0 41.0 32.0 41.0 30 36.84453966516208 39.0 35.0 41.0 32.0 41.0 31 36.64230402230259 39.0 35.0 41.0 31.0 41.0 32 36.354916828940716 39.0 35.0 41.0 30.0 41.0 33 35.99812031312162 39.0 35.0 41.0 29.0 41.0 34 35.62586035158879 39.0 35.0 41.0 25.0 41.0 35 35.3684966991813 39.0 35.0 41.0 23.0 41.0 36 35.227550394692365 39.0 35.0 41.0 23.0 41.0 37 35.12050366275713 39.0 35.0 41.0 22.0 41.0 38 35.03921976841923 39.0 35.0 41.0 22.0 41.0 39 34.92149196406938 39.0 35.0 41.0 21.0 41.0 40 34.80732252303992 39.0 35.0 41.0 20.0 41.0 41 34.71499083189007 38.0 34.0 41.0 20.0 41.0 42 34.648731271181624 38.0 34.0 41.0 18.0 41.0 43 34.54038455210866 38.0 34.0 41.0 18.0 41.0 44 34.420260775146495 38.0 34.0 40.0 18.0 41.0 45 34.38993332555614 38.0 34.0 40.0 18.0 41.0 46 34.31987430865272 38.0 34.0 40.0 18.0 41.0 47 34.24080452034184 38.0 34.0 40.0 18.0 41.0 48 34.182173186045326 38.0 34.0 40.0 18.0 41.0 49 34.11634884882639 38.0 34.0 40.0 18.0 41.0 50 33.95018769947743 38.0 33.0 40.0 18.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 1.0 9 1.0 10 8.0 11 7.0 12 17.0 13 30.0 14 37.0 15 76.0 16 211.0 17 488.0 18 1180.0 19 2436.0 20 4302.0 21 7558.0 22 12044.0 23 18565.0 24 27533.0 25 43620.0 26 66987.0 27 82531.0 28 81116.0 29 73634.0 30 71764.0 31 77169.0 32 90522.0 33 115246.0 34 188115.0 35 305063.0 36 211405.0 37 265812.0 38 465565.0 39 1130066.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.625100579998865 22.27485784194956 26.435385015748807 18.664656562302767 2 35.83441765302368 24.589528911701976 27.91649093209616 11.659562503178178 3 26.359467681290777 24.665805193367852 36.89755945810936 12.077167667232008 4 23.15918411299658 25.66996000729859 38.45829183006242 12.712564049642399 5 21.951655793557496 28.533461357837464 36.48435139735157 13.030531451253474 6 19.767432121587383 37.74883267376784 33.63469942658184 8.849035778062941 7 75.12597551381796 5.3692818961984505 16.970365916766124 2.5343766732174533 8 74.71040438394189 5.903903850007927 16.368740484159957 3.016951281890216 9 68.1431062693121 7.860465255405895 19.131287932493997 4.865140542788002 10 31.953360792794733 30.62465787844253 26.15657277205955 11.26540855670319 11 27.3240186532899 24.481575538944274 35.12788989892645 13.066515908839374 12 25.347416028787567 23.333423070135293 36.12812620583828 15.191034695238864 13 18.974667300806733 28.61820281115488 36.99061652174173 15.416513366296652 14 14.404192503387565 31.7326082599735 37.76235301859646 16.10084621804248 15 13.885334314455703 25.852185539811735 45.485939738746254 14.77654040698631 16 16.235840280457417 25.405386002853632 41.926020980464294 16.432752736224653 17 15.728617963513017 25.30239806647104 38.14035434071867 20.82862962929727 18 17.59281028742698 26.163033821800656 39.526578545127144 16.717577345645225 19 19.85316067972636 26.06946824962385 37.00168406065011 17.075687009999672 20 20.839039098324612 25.499370346772913 37.98364397222945 15.67794658267302 21 18.003834752670418 27.63498658434811 38.723972588398226 15.637206074583249 22 18.12874838099853 25.585158729446533 36.66083377453928 19.62525911501566 23 16.770013550257097 27.328086721645413 37.72831285838635 18.173586869711137 24 14.98155908719725 26.47636482197714 42.65429495290313 15.887781137922474 25 15.616237575191963 28.914334257622393 38.682334712288856 16.787093454896787 26 15.77504180239358 29.724597754785215 36.77210740896949 17.72825303385171 27 15.645910544373354 30.583678072214195 36.94981618911732 16.820595194295134 28 14.41977679466126 28.771951865179428 40.11342731767725 16.694844022482062 29 15.188761362922548 26.25779588467026 40.13756651740445 18.415876235002738 30 16.23554115778422 30.312912228434 36.72750821839544 16.72403839538633 31 18.423503863169323 29.463344012012765 34.78751820909273 17.325633915725177 32 19.238014902291578 27.939313992061283 35.965223998013826 16.857447107633313 33 19.344323100346685 27.849307979695553 34.98033268423713 17.826036235720633 34 16.171169958511687 28.10604497010269 35.53714954039801 20.185635530987614 35 17.417255190526188 27.338795313345955 35.93806365928731 19.30588583684055 36 19.852771820251203 29.958810807900427 32.85180565401677 17.336611717831598 37 16.673755874021495 30.96837974221608 34.83247634687462 17.525388036887808 38 16.62790036822001 30.786632805980062 34.37562628809701 18.209840537702917 39 17.485156037342474 29.780952466416 34.183918566843445 18.549972929398077 40 17.97245678425179 28.42999482517775 34.34469700368818 19.252851386882273 41 15.925350945676332 29.397297725770315 33.19292515053348 21.484426178019866 42 17.945864778604353 29.070625854369137 32.189907002760904 20.79360236426561 43 18.017953342845434 29.360685110570696 33.059247227880626 19.562114318703244 44 16.353545052361422 30.402260170918694 33.33662368273853 19.907571093981353 45 16.491560253775674 31.662164870435017 32.2651662673379 19.581108608451412 46 18.60692588637526 31.50898414948955 31.84181794795864 18.042272016176554 47 16.668401578171224 30.113457229944572 34.59383627819605 18.624304913688153 48 16.913831731531417 29.558674407961448 34.200729261077264 19.326764599429872 49 17.473191130414495 28.381536952119436 34.69236728674797 19.452904630718105 50 16.073476493444726 30.18162728716674 34.10976605615729 19.635130163231242 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5568.0 1 10915.0 2 16262.0 3 54337.5 4 92413.0 5 65054.0 6 37695.0 7 38343.0 8 38991.0 9 39865.5 10 40740.0 11 39901.5 12 39063.0 13 37313.5 14 35564.0 15 34029.5 16 32495.0 17 33293.0 18 34091.0 19 33071.0 20 32051.0 21 37274.0 22 42497.0 23 47175.5 24 51854.0 25 63043.5 26 74233.0 27 82714.5 28 91196.0 29 117151.0 30 143106.0 31 155620.0 32 168134.0 33 182963.5 34 197793.0 35 201786.5 36 205780.0 37 207072.5 38 208365.0 39 214812.0 40 221259.0 41 221849.5 42 222440.0 43 213863.5 44 205287.0 45 190053.0 46 174819.0 47 180833.5 48 186848.0 49 177232.0 50 167616.0 51 152925.5 52 138235.0 53 118707.0 54 99179.0 55 85364.0 56 71549.0 57 66866.0 58 62183.0 59 58856.5 60 55530.0 61 50663.0 62 45796.0 63 40531.5 64 35267.0 65 29460.5 66 23654.0 67 19498.0 68 15342.0 69 13679.0 70 12016.0 71 9888.0 72 7760.0 73 6451.5 74 5143.0 75 3985.0 76 2827.0 77 2058.0 78 1289.0 79 943.0 80 597.0 81 441.5 82 286.0 83 217.5 84 149.0 85 103.0 86 57.0 87 46.5 88 36.0 89 25.0 90 14.0 91 23.5 92 33.0 93 18.5 94 4.0 95 3.0 96 2.0 97 2.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3343110.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.500398258064234 #Duplication Level Percentage of deduplicated Percentage of total 1 78.70931217970909 16.135722463023424 2 8.315726764714306 3.4095142096377455 3 3.043223793605658 1.8716189927199918 4 1.624394716134715 1.3320295443622743 5 1.006833725934255 1.0320246180651462 6 0.7179674412015011 0.8831171088572454 7 0.5279599392798704 0.7576372313678528 8 0.4107199827702463 0.6735938575468263 9 0.3478264972150383 0.6417523545854179 >10 3.812167497732657 18.289076453574104 >50 0.7938161573370437 11.168739057978367 >100 0.5897651477660755 25.750048850544328 >500 0.08403950383675848 11.684753201331274 >1k 0.01580356223292295 4.316827624749297 >5k 2.9539368659669066E-4 0.4542371732434292 >10k+ 1.4769684329834533E-4 1.5993072584134072 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 52820 1.5799659598397897 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCC 8706 0.2604161992874898 No Hit GAATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTT 6296 0.1883276350464089 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATG 4158 0.12437520751635454 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3437 0.10280846277867016 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.05566672948242804 0.0 2 0.0 0.0 0.0 0.240315155648483 0.0 3 0.0 0.0 0.0 0.34300396935787336 0.0 4 0.0 0.0 0.0 0.5751829882953298 0.0 5 0.0 0.0 0.0 1.1730095629518622 0.0 6 0.0 0.0 0.0 1.5800856089090698 0.0 7 0.0 0.0 0.0 1.8782810018216571 0.0 8 0.0 0.0 0.0 2.492290113098283 0.0 9 2.9912267319950587E-5 0.0 0.0 2.7227043082638622 0.0 10 2.9912267319950587E-5 0.0 0.0 3.386846379568725 0.0 11 2.9912267319950587E-5 0.0 0.0 3.8031054915931573 0.0 12 2.9912267319950587E-5 0.0 0.0 4.292739395353428 0.0 13 2.9912267319950587E-5 0.0 0.0 4.4481635363478915 0.0 14 2.9912267319950587E-5 0.0 0.0 4.510081929700189 0.0 15 2.9912267319950587E-5 0.0 0.0 4.632602576642707 0.0 16 5.982453463990117E-5 0.0 0.0 4.839565554229445 0.0 17 5.982453463990117E-5 0.0 0.0 5.064804927148673 0.0 18 5.982453463990117E-5 0.0 0.0 5.370029702881449 0.0 19 5.982453463990117E-5 0.0 0.0 5.564130405520608 0.0 20 5.982453463990117E-5 0.0 0.0 5.7449500614697095 0.0 21 5.982453463990117E-5 0.0 0.0 5.991666442324662 0.0 22 5.982453463990117E-5 0.0 0.0 6.2574967619970625 0.0 23 8.973680195985175E-5 0.0 0.0 6.544714352803228 0.0 24 8.973680195985175E-5 0.0 0.0 6.764868640278064 0.0 25 8.973680195985175E-5 0.0 0.0 6.959657325065582 0.0 26 8.973680195985175E-5 0.0 0.0 7.162971006039287 0.0 27 8.973680195985175E-5 0.0 0.0 7.369844246824065 0.0 28 8.973680195985175E-5 0.0 0.0 7.5784523991134005 0.0 29 8.973680195985175E-5 0.0 0.0 7.81487896001029 0.0 30 8.973680195985175E-5 0.0 0.0 8.103233216974614 0.0 31 8.973680195985175E-5 0.0 0.0 8.37737914696196 0.0 32 8.973680195985175E-5 0.0 0.0 8.627206403618187 0.0 33 8.973680195985175E-5 0.0 0.0 8.865068753346435 0.0 34 8.973680195985175E-5 0.0 0.0 9.131527230632555 0.0 35 8.973680195985175E-5 0.0 0.0 9.434927358058813 0.0 36 8.973680195985175E-5 0.0 0.0 9.688523560397355 0.0 37 8.973680195985175E-5 0.0 0.0 9.937363712232024 0.0 38 8.973680195985175E-5 0.0 0.0 10.190750528699326 2.9912267319950587E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGATC 25 4.4446693E-5 44.0 26 CGCCGAT 20 7.858642E-4 44.0 30 TATCGTG 25 4.4446693E-5 44.0 1 CGTTTTT 30275 0.0 42.299587 1 ATCGTAC 55 7.8216544E-11 40.0 44 GCGTAAG 160 0.0 39.875 1 CGCACTA 145 0.0 39.448277 43 CGGTCTA 235 0.0 39.319145 31 TGCGTTG 180 0.0 39.11111 1 CCCTACA 1435 0.0 38.787457 27 AACGTGC 205 0.0 38.634148 11 GGCGATT 860 0.0 38.627907 8 TAACGCG 40 4.127687E-7 38.5 1 CGCAATA 80 0.0 38.5 30 ACGGGAT 1460 0.0 38.424656 5 TAACGGG 665 0.0 38.37594 3 TTATCGT 75 0.0 38.13333 40 TACGGGA 1480 0.0 37.905403 4 AAGGGAT 4690 0.0 37.855015 5 AGGGCGA 1360 0.0 37.852943 6 >>END_MODULE