FastQCFastQC Report
Thu 26 May 2016
SRR1546169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546169_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1849078
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294621.593334624066697No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA142370.7699512946452232No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA118300.6397783111366854No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA69340.3749977015572085No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA64180.347091902018195No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA48410.2618061542022565No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG40810.22070458898975598No Hit
AGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG32630.1764663253794594No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26470.14315242515459056No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA25030.1353647601669589No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA25020.1353106791601003No Hit
AGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG24910.13471578808465626No Hit
GGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA24240.1310923606251332No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA22080.11941086314368567No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA21950.11870781005452447No Hit
TAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA20130.10886506680626777No Hit
AAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA19630.1061610164633401No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAG302.5285553E-644.0000041
ATCGATC600.044.00000428
TAATACG207.857727E-444.01
GTCGATT254.443896E-544.031
GCCGGTT207.857727E-444.017
CGTTAGG1800.044.02
TGCATCG254.443896E-544.034
CCCGTTG207.857727E-444.023
TACGCAC454.802132E-1044.011
TCTAACG207.857727E-444.01
TATTACG502.7284841E-1144.01
ATGCGTC207.857727E-444.028
TATAGCG950.044.01
ATACGTC207.857727E-444.023
GCGTCCA207.857727E-444.030
CATCTCG351.4467332E-743.99999623
TCGCTAA351.4467332E-743.99999627
TAATGCG1700.042.705881
GCGATAA1100.042.09
CGTTTTT187400.041.8516541
Produced by FastQC (version 0.11.5)