##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546169_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1849078
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97462140591149	33.0	31.0	34.0	30.0	34.0
2	32.33217041141585	33.0	31.0	34.0	31.0	34.0
3	32.254836194038326	34.0	31.0	34.0	30.0	34.0
4	35.98779878404264	37.0	35.0	37.0	35.0	37.0
5	35.966418398791184	37.0	35.0	37.0	35.0	37.0
6	36.022959009841664	37.0	35.0	37.0	35.0	37.0
7	36.20471608012209	37.0	35.0	37.0	35.0	37.0
8	36.08628408320255	37.0	35.0	37.0	35.0	37.0
9	37.74092926312465	39.0	38.0	39.0	35.0	39.0
10	37.592826803412294	39.0	37.0	39.0	35.0	39.0
11	37.48077149801144	39.0	37.0	39.0	35.0	39.0
12	37.220780302399355	39.0	37.0	39.0	34.0	39.0
13	37.12636027252501	39.0	37.0	39.0	33.0	39.0
14	38.41630693783605	40.0	38.0	41.0	34.0	41.0
15	38.476423385060016	40.0	37.0	41.0	34.0	41.0
16	38.49055529296222	40.0	37.0	41.0	34.0	41.0
17	38.414446010390044	40.0	37.0	41.0	34.0	41.0
18	38.21255890773672	40.0	37.0	41.0	34.0	41.0
19	38.005576292617185	40.0	36.0	41.0	34.0	41.0
20	37.77837495227351	40.0	35.0	41.0	34.0	41.0
21	37.71840073809758	40.0	35.0	41.0	34.0	41.0
22	37.63704018975944	39.0	35.0	41.0	33.0	41.0
23	37.58489095646587	39.0	35.0	41.0	33.0	41.0
24	37.50901638546346	39.0	35.0	41.0	33.0	41.0
25	37.49024432717279	39.0	35.0	41.0	33.0	41.0
26	37.41330490114533	39.0	35.0	41.0	33.0	41.0
27	37.35519269603554	39.0	35.0	41.0	33.0	41.0
28	37.272669946860006	39.0	35.0	41.0	33.0	41.0
29	37.166803131073976	39.0	35.0	41.0	33.0	41.0
30	36.99771237340988	39.0	35.0	41.0	33.0	41.0
31	36.78661797933889	39.0	35.0	41.0	32.0	41.0
32	36.485736134441055	39.0	35.0	41.0	31.0	41.0
33	36.09929002454196	39.0	35.0	41.0	29.0	41.0
34	35.678177989246535	39.0	35.0	41.0	25.0	41.0
35	35.42536604729492	39.0	35.0	41.0	23.0	41.0
36	35.239606982506956	39.0	35.0	41.0	22.0	41.0
37	35.17024484635045	39.0	35.0	41.0	22.0	41.0
38	35.10502910098979	39.0	35.0	41.0	21.0	41.0
39	35.03264113249955	39.0	35.0	41.0	21.0	41.0
40	34.88723190692875	39.0	35.0	41.0	20.0	41.0
41	34.81359899366063	39.0	35.0	41.0	19.0	41.0
42	34.757065953951106	39.0	35.0	41.0	18.0	41.0
43	34.64155379059185	39.0	35.0	41.0	18.0	41.0
44	34.50443031608185	38.0	34.0	41.0	18.0	41.0
45	34.442118179979424	38.0	34.0	40.0	18.0	41.0
46	34.39053896049815	38.0	34.0	40.0	18.0	41.0
47	34.39202023927601	38.0	34.0	40.0	18.0	41.0
48	34.284380648085154	38.0	34.0	40.0	18.0	41.0
49	34.15224776888806	38.0	34.0	40.0	18.0	41.0
50	34.05437899320634	38.0	34.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	12.0
12	7.0
13	11.0
14	18.0
15	45.0
16	91.0
17	219.0
18	530.0
19	1098.0
20	2075.0
21	3470.0
22	5661.0
23	8801.0
24	14077.0
25	23830.0
26	38492.0
27	47918.0
28	45422.0
29	38932.0
30	36531.0
31	38520.0
32	45543.0
33	58081.0
34	97864.0
35	180464.0
36	108796.0
37	138994.0
38	244924.0
39	668650.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.07438193521312	24.552236303714608	27.330377626038487	19.043004135033783
2	35.571133289131126	27.719490470385782	26.892537794511647	9.81683844597145
3	27.64139749648203	24.805930306888083	37.21508773561742	10.337584461012462
4	26.398561877865617	26.426305434384055	36.25871921033077	10.916413477419558
5	21.920600428970545	31.049852953742352	35.78897158475738	11.240575032529726
6	19.132886768432698	40.50202317046658	32.40858417005664	7.956505891044078
7	75.85710283719779	6.992079295735497	15.451376307543544	1.6994415595231787
8	75.99214311132359	7.038048151565267	15.105960916737962	1.8638478203731805
9	67.34653703088782	12.403641166029773	17.138162911461823	3.1116588916205807
10	31.34443219810089	37.577051914521725	23.039860946915166	8.03865494046222
11	31.470981754149907	23.65005694730022	33.06848061574471	11.81048068280516
12	31.655992878613016	24.447265069402153	32.283602963206526	11.613139088778299
13	18.843661543753157	34.69631892218716	31.8152073628046	14.644812171255078
14	13.931105123742752	37.28571753057469	32.8319302917454	15.95124705393715
15	16.257291471749706	28.628916681719215	42.314115467276125	12.79967637925496
16	16.66381840030545	26.684974890188517	39.64727285706714	17.00393385243889
17	15.72367417707636	29.366419372249304	32.95864209081499	21.951264359859344
18	16.178657687777367	32.13590773347582	36.56984724278803	15.115587335958786
19	22.11334513741443	28.354347409898338	33.40459407337062	16.12771337931661
20	21.790643769489442	29.036308906384694	35.80032859619767	13.37271872792819
21	18.026281206092985	32.476509914670984	33.89613634470801	15.601072534528019
22	19.09097398811732	30.612067203222367	31.66351013856635	18.633448670093962
23	17.850572014809543	31.25963317934668	32.73636915262634	18.153425653217443
24	15.32661142472086	29.03939152377563	42.52611301416165	13.107884037341854
25	14.9124049931912	35.25589510015262	35.26341236010595	14.568287546550227
26	14.892395020653538	35.5301939669392	32.67439231876643	16.90301869364083
27	17.126914062035244	33.72253631269205	33.60480196076098	15.545747664511719
28	14.416644403318843	33.57949204955118	37.710091191393765	14.293772355736209
29	14.386304958471193	30.506284753807034	36.08744466160973	19.01996562611204
30	14.58754038499187	36.8235412459615	33.61031822346055	14.978600145586071
31	20.896035754035257	33.70220185411324	31.631494182506092	13.770268209345415
32	19.96346287176636	32.382733448778254	31.863069053874415	15.790734625580965
33	18.980973220167023	35.74808634357231	29.360416380488008	15.91052405577266
34	16.572691903748787	31.733599123455043	33.99964739183528	17.69406158096089
35	22.16985978958162	29.87040027516416	31.37244615965362	16.5872937756006
36	21.820388323261646	34.89901453589302	29.071894208897625	14.208702931947705
37	16.808106526604067	36.90266175899556	32.441735827260935	13.847495887139427
38	15.593501193567821	35.620130681344975	33.52595185276122	15.26041627232599
39	19.709444382551737	32.21205379113266	32.72106422768537	15.357437598630236
40	18.186901796462887	33.399726782753355	32.11898037832909	16.294391042454674
41	15.345269372087062	33.72242815067834	32.660006770942054	18.27229570629254
42	16.61784954447568	33.634438352519474	29.77456873101081	19.97314337199404
43	19.944101871311	31.620083090058937	30.36351089570045	18.072304142929614
44	17.60131265419847	34.61465660183075	29.080871656036145	18.703159087934633
45	15.838650397657645	39.69567535820555	27.26688652398655	17.198787720150257
46	17.72656426608288	38.296004819699334	29.150636154883674	14.826794759334112
47	19.52513631117779	32.17495422042769	31.811205368297063	16.488704100097454
48	17.971280822117834	31.577088689606388	33.351594686649236	17.10003580162654
49	17.29991920297575	34.59237522700503	31.54355846535408	16.564147104665135
50	16.130471510666396	34.960991369752925	31.427987353697358	17.48054976588332
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4388.0
1	7414.0
2	10440.0
3	29831.0
4	49222.0
5	35912.0
6	22602.0
7	22698.0
8	22794.0
9	23400.0
10	24006.0
11	24173.5
12	24341.0
13	22775.0
14	21209.0
15	19906.0
16	18603.0
17	16942.0
18	15281.0
19	17720.0
20	20159.0
21	18062.0
22	15965.0
23	21139.0
24	26313.0
25	33461.0
26	40609.0
27	46859.0
28	53109.0
29	80027.5
30	106946.0
31	113840.0
32	120734.0
33	121922.5
34	123111.0
35	125221.5
36	127332.0
37	115331.5
38	103331.0
39	112692.0
40	122053.0
41	125347.0
42	128641.0
43	124392.0
44	120143.0
45	109885.0
46	99627.0
47	92725.0
48	85823.0
49	84459.0
50	83095.0
51	75790.5
52	68486.0
53	55687.5
54	42889.0
55	36944.5
56	31000.0
57	28845.0
58	26690.0
59	27066.5
60	27443.0
61	24954.0
62	22465.0
63	19056.0
64	15647.0
65	12343.0
66	9039.0
67	7225.5
68	5412.0
69	4590.0
70	3768.0
71	3392.5
72	3017.0
73	2353.0
74	1689.0
75	1256.0
76	823.0
77	560.0
78	297.0
79	288.5
80	280.0
81	226.0
82	172.0
83	104.0
84	36.0
85	38.0
86	40.0
87	21.0
88	2.0
89	3.0
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1849078.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.26799553242576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.95149422219887	16.791400263960643
2	8.030018196038165	3.415647822372745
3	2.9368208956913944	1.873808810672976
4	1.5278671345929145	1.2997868557064902
5	1.0197259699704357	1.0843763686814878
6	0.7335914045511901	0.9361211228772389
7	0.5831726890470308	0.8682039901699478
8	0.4594814622895005	0.7817799749764441
9	0.39018930650961237	0.7468689984882069
>10	4.088407964134901	19.55763205223928
>50	0.6462612622783516	9.514551492925458
>100	0.5461213783099611	24.835420131984048
>500	0.072543719076791	10.02603338219059
>1k	0.013027217158156128	4.526336875246438
>5k	5.108712611041619E-4	0.7253612659724241
>10k+	7.663068916562428E-4	3.016670591535555
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29462	1.593334624066697	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	14237	0.7699512946452232	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	11830	0.6397783111366854	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	6934	0.3749977015572085	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	6418	0.347091902018195	No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4841	0.2618061542022565	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4081	0.22070458898975598	No Hit
AGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3263	0.1764663253794594	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2647	0.14315242515459056	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2503	0.1353647601669589	No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2502	0.1353106791601003	No Hit
AGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2491	0.13471578808465626	No Hit
GGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	2424	0.1310923606251332	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	2208	0.11941086314368567	No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2195	0.11870781005452447	No Hit
TAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2013	0.10886506680626777	No Hit
AAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1963	0.1061610164633401	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0509443084607572	0.0
2	0.0	0.0	0.0	0.21340365306385128	0.0
3	0.0	0.0	0.0	0.31150659950526693	0.0
4	0.0	0.0	0.0	0.46807111436077875	0.0
5	0.0	0.0	0.0	0.8005070635203058	0.0
6	0.0	0.0	0.0	1.106659643346576	0.0
7	0.0	0.0	0.0	1.2946452231869072	0.0
8	0.0	0.0	0.0	1.7540633764503173	0.0
9	0.0	0.0	0.0	1.954109020820106	0.0
10	0.0	0.0	0.0	2.355552334731147	0.0
11	0.0	0.0	0.0	2.8316815191138502	0.0
12	0.0	0.0	0.0	3.19056308062721	0.0
13	0.0	0.0	0.0	3.338041986330485	0.0
14	0.0	0.0	0.0	3.3992076050875086	0.0
15	0.0	0.0	0.0	3.4796801432930358	0.0
16	0.0	0.0	0.0	3.6706401785105873	0.0
17	0.0	0.0	0.0	3.882096915327531	0.0
18	0.0	0.0	0.0	4.148175469071613	0.0
19	0.0	0.0	0.0	4.294518673630858	0.0
20	0.0	0.0	0.0	4.452002565602966	0.0
21	0.0	0.0	0.0	4.6653521376599585	0.0
22	0.0	0.0	0.0	4.885191430539977	0.0
23	0.0	0.0	0.0	5.11346736048993	0.0
24	0.0	0.0	0.0	5.300263158179374	0.0
25	0.0	0.0	0.0	5.475918268455954	0.0
26	0.0	0.0	0.0	5.635240914661253	0.0
27	0.0	0.0	0.0	5.801540010751304	0.0
28	0.0	0.0	0.0	5.972652316451767	0.0
29	0.0	0.0	0.0	6.152958393318183	0.0
30	0.0	0.0	0.0	6.386047532878548	0.0
31	0.0	0.0	0.0	6.602912370381347	0.0
32	0.0	0.0	0.0	6.817505805596086	0.0
33	0.0	0.0	0.0	7.011115810149707	0.0
34	0.0	0.0	0.0	7.222680708980367	0.0
35	0.0	0.0	0.0	7.479835896592788	0.0
36	0.0	0.0	0.0	7.713303603201163	0.0
37	0.0	0.0	0.0	7.936171432465261	0.0
38	0.0	0.0	0.0	8.144275146856975	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAG	30	2.5285553E-6	44.000004	1
ATCGATC	60	0.0	44.000004	28
TAATACG	20	7.857727E-4	44.0	1
GTCGATT	25	4.443896E-5	44.0	31
GCCGGTT	20	7.857727E-4	44.0	17
CGTTAGG	180	0.0	44.0	2
TGCATCG	25	4.443896E-5	44.0	34
CCCGTTG	20	7.857727E-4	44.0	23
TACGCAC	45	4.802132E-10	44.0	11
TCTAACG	20	7.857727E-4	44.0	1
TATTACG	50	2.7284841E-11	44.0	1
ATGCGTC	20	7.857727E-4	44.0	28
TATAGCG	95	0.0	44.0	1
ATACGTC	20	7.857727E-4	44.0	23
GCGTCCA	20	7.857727E-4	44.0	30
CATCTCG	35	1.4467332E-7	43.999996	23
TCGCTAA	35	1.4467332E-7	43.999996	27
TAATGCG	170	0.0	42.70588	1
GCGATAA	110	0.0	42.0	9
CGTTTTT	18740	0.0	41.851654	1
>>END_MODULE