FastQCFastQC Report
Thu 26 May 2016
SRR1546168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546168_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1888999
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299801.5870839529295675No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA146310.7745372019784023No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA119900.6347277049908444No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA69620.36855498600052194No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA64770.34288001211223507No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA48290.2556380389825511No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG41230.21826374709568402No Hit
AGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG33020.17480157480231595No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27510.1456326869416024No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA27160.14377985377440644No Hit
AGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG26400.13975655889706665No Hit
GGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA26000.1376390352774141No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA25200.13340398803810907No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA22760.12048709395822868No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA22270.11789312752415433No Hit
TAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA21400.11328751365141009No Hit
AAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA20270.10730550942589169No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA19280.1020646384672517No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCGT650.044.0000045
TAATACG408.3182385E-944.01
TACCCGC502.7284841E-1144.030
CTCCGTA207.85777E-444.017
ACGTTAG351.4467514E-744.01
CGCATTA207.85777E-444.021
GCACGAG551.8189894E-1244.01
CTAAGCG502.7284841E-1144.01
ACTTACC302.5285863E-644.042
TAGCACG207.85777E-444.01
AACGGAT207.85777E-444.017
CTAACGG302.5285863E-644.02
TATCTCG207.85777E-444.01
TCGCTAA351.4467514E-744.027
GTCGTAG600.044.01
TTAACGA302.5285863E-644.040
CGTAATC502.7284841E-1144.038
TATAGCG1100.044.01
GGACCGT600.044.08
ATACGAC254.443932E-544.024
Produced by FastQC (version 0.11.5)