##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546168_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1888999
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91094701479461	31.0	31.0	34.0	30.0	34.0
2	32.30154330415209	33.0	31.0	34.0	31.0	34.0
3	32.34198747590655	34.0	31.0	34.0	30.0	34.0
4	36.02337957828458	37.0	35.0	37.0	35.0	37.0
5	35.92592161245189	37.0	35.0	37.0	35.0	37.0
6	36.01000635786467	37.0	35.0	37.0	35.0	37.0
7	36.186984746947985	37.0	35.0	37.0	35.0	37.0
8	36.06521284553354	37.0	35.0	37.0	35.0	37.0
9	37.71576480453404	39.0	38.0	39.0	35.0	39.0
10	37.59464933544168	39.0	37.0	39.0	35.0	39.0
11	37.48201878349327	39.0	37.0	39.0	35.0	39.0
12	37.20944796688617	39.0	37.0	39.0	34.0	39.0
13	37.091701477872675	39.0	37.0	39.0	33.0	39.0
14	38.37063492357593	40.0	37.0	41.0	33.0	41.0
15	38.456164349478215	40.0	37.0	41.0	34.0	41.0
16	38.45510876395382	40.0	37.0	41.0	34.0	41.0
17	38.3886799304817	40.0	37.0	41.0	34.0	41.0
18	38.19605410061096	40.0	37.0	41.0	34.0	41.0
19	37.99584806556277	40.0	36.0	41.0	34.0	41.0
20	37.76590882260923	40.0	35.0	41.0	34.0	41.0
21	37.70113430446496	40.0	35.0	41.0	34.0	41.0
22	37.63114273750277	39.0	35.0	41.0	33.0	41.0
23	37.56562338042529	39.0	35.0	41.0	33.0	41.0
24	37.49531630244378	39.0	35.0	41.0	33.0	41.0
25	37.469155356884784	39.0	35.0	41.0	33.0	41.0
26	37.41093510372425	39.0	35.0	41.0	33.0	41.0
27	37.34765979230269	39.0	35.0	41.0	33.0	41.0
28	37.25467244821199	39.0	35.0	41.0	33.0	41.0
29	37.15281320953584	39.0	35.0	41.0	33.0	41.0
30	36.98532238503038	39.0	35.0	41.0	33.0	41.0
31	36.78020316580369	39.0	35.0	41.0	32.0	41.0
32	36.48825065550591	39.0	35.0	41.0	31.0	41.0
33	36.084319790534565	39.0	35.0	41.0	29.0	41.0
34	35.71693261881028	39.0	35.0	41.0	25.0	41.0
35	35.448922418699006	39.0	35.0	41.0	23.0	41.0
36	35.25860521895459	39.0	35.0	41.0	23.0	41.0
37	35.10666866419728	39.0	35.0	41.0	22.0	41.0
38	35.054199075806814	39.0	35.0	41.0	21.0	41.0
39	34.99598888088347	39.0	35.0	41.0	21.0	41.0
40	34.87073524125741	39.0	35.0	41.0	20.0	41.0
41	34.816797679617615	39.0	35.0	41.0	19.0	41.0
42	34.73984740066035	39.0	35.0	41.0	18.0	41.0
43	34.6542147454816	39.0	35.0	41.0	18.0	41.0
44	34.513742992981996	38.0	34.0	41.0	18.0	41.0
45	34.446759897702435	38.0	34.0	41.0	18.0	41.0
46	34.40211720599111	38.0	34.0	40.0	18.0	41.0
47	34.38972175210257	38.0	34.0	40.0	18.0	41.0
48	34.30067300194442	38.0	34.0	40.0	18.0	41.0
49	34.16659034758621	38.0	34.0	40.0	18.0	41.0
50	34.04560192991103	38.0	34.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	2.0
11	5.0
12	4.0
13	8.0
14	13.0
15	34.0
16	77.0
17	238.0
18	545.0
19	1176.0
20	2057.0
21	3458.0
22	5818.0
23	9156.0
24	14119.0
25	24497.0
26	38861.0
27	48452.0
28	46263.0
29	40287.0
30	38042.0
31	39754.0
32	47140.0
33	60515.0
34	102055.0
35	181931.0
36	112311.0
37	142522.0
38	251221.0
39	678435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.21150302355904	24.551045289065794	27.364651860588594	18.87279982678657
2	35.63035237181174	27.673439742424428	26.904566916128598	9.79164096963524
3	27.63606545053756	24.796572152764508	37.19705515990215	10.370307236795783
4	26.367086483370294	26.4968377431645	36.26338605790686	10.872689715558346
5	21.932197952460537	31.04326683073946	35.7573508509004	11.267184365899611
6	19.149771916237118	40.4296137795732	32.45692559921948	7.963688704970198
7	75.81655681130587	6.999897829485352	15.477509516945217	1.706035842263548
8	75.94471992838535	7.079781408036744	15.10429597898146	1.8712026845964451
9	67.33508064324015	12.418164329361742	17.133624739875458	3.113130287522651
10	31.386305657123163	37.581226882597605	22.998953413950986	8.033514046328241
11	31.44750208973112	23.69853027979369	32.9754012574914	11.878566372983787
12	31.593505343306166	24.451839307485077	32.3373913908901	11.617263958318665
13	18.924361526925107	34.675190405076975	31.82754464136826	14.57290342662966
14	13.947810454108234	37.2540694833613	32.87958331370213	15.918536748828346
15	16.334894830542527	28.6157377531698	42.316380262774096	12.73298715351358
16	16.757129040301237	26.67492147957728	39.67604006142936	16.891909418692123
17	15.779838951741107	29.34008964536244	32.937074079975694	21.942997322920764
18	16.21292547005054	32.114469091831175	36.575244348991184	15.097361089127098
19	22.14993231865131	28.395356482454464	33.37958357839258	16.075127620501654
20	21.770419147919082	29.087627891809365	35.72834077731116	13.413612182960394
21	18.082963516656175	32.4317800062361	33.96740813520812	15.517848341899597
22	18.975499722339713	30.696945842745283	31.674130055124433	18.653424379790568
23	17.920655331209808	31.18567029416109	32.75205545370855	18.141618920920553
24	15.412554479912377	29.1209259507284	42.31124526799643	13.155274301362784
25	14.931929556341744	35.181913807259825	35.33156978907876	14.554586847319664
26	14.907419220444268	35.57010882483262	32.665131109121816	16.857340845601296
27	17.23579525452369	33.70097072576534	33.616163904798256	15.447070114912714
28	14.424888525615948	33.63733914099478	37.64798181470715	14.289790518682116
29	14.4830674870659	30.556289336309867	35.995889886654254	18.96475328996998
30	14.6579749380492	36.732788106293334	33.58207177452185	15.027165181135619
31	20.787147055133435	33.74898557384096	31.6383968440428	13.825470526982809
32	19.958401248491924	32.41616326954117	31.853484305709003	15.7719511762579
33	19.019914780261928	35.60510090264738	29.436278155785157	15.938706161305538
34	16.65728780163462	31.79980508195081	33.897529855759586	17.645377260654982
35	22.09921762796063	29.944219134049305	31.30996893063469	16.64659430735538
36	21.932886147636925	34.85735037445758	28.961846988802005	14.247916489103488
37	16.855435074343607	36.95687504334306	32.34670849481657	13.840981387496765
38	15.646434963703	35.6604212072108	33.40615849981921	15.286985329266983
39	19.78529369258533	32.21632197793646	32.579212588254414	15.419171741223792
40	18.198633244379696	33.28884769129047	32.17915943841156	16.333359625918277
41	15.422506840924743	33.68535398907041	32.56026075185852	18.33187841814633
42	16.69265044608282	33.58164826979792	29.800809846908336	19.924891437210928
43	19.969941752219032	31.61605696985546	30.343478212534787	18.070523065390717
44	17.598844679113117	34.6585678446627	29.054859213795243	18.687728262428937
45	15.940506056382242	39.57572237994832	27.261157893678078	17.222613669991354
46	17.796991951822104	38.25618753636185	29.12087301263791	14.825947499178135
47	19.592228476563513	32.16555434915529	31.754860643123685	16.487356531157506
48	18.004615142729033	31.60832800864373	33.291971038629455	17.09508580999778
49	17.404932453643436	34.510129438925055	31.51473346465509	16.570204642776414
50	16.22091912171473	34.970373197656535	31.353325226747074	17.45538245388166
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4490.0
1	7814.5
2	11139.0
3	30774.0
4	50409.0
5	36662.0
6	22915.0
7	22964.5
8	23014.0
9	23574.0
10	24134.0
11	24385.5
12	24637.0
13	23123.0
14	21609.0
15	20331.0
16	19053.0
17	17223.5
18	15394.0
19	17916.0
20	20438.0
21	18308.5
22	16179.0
23	21279.5
24	26380.0
25	34131.0
26	41882.0
27	48426.5
28	54971.0
29	81845.0
30	108719.0
31	115867.0
32	123015.0
33	123873.0
34	124731.0
35	127172.0
36	129613.0
37	117507.0
38	105401.0
39	115021.5
40	124642.0
41	127690.0
42	130738.0
43	126692.0
44	122646.0
45	112366.5
46	102087.0
47	95366.0
48	88645.0
49	87645.5
50	86646.0
51	78870.0
52	71094.0
53	57590.0
54	44086.0
55	38118.5
56	32151.0
57	29836.0
58	27521.0
59	27482.5
60	27444.0
61	25115.5
62	22787.0
63	19277.0
64	15767.0
65	12437.5
66	9108.0
67	7322.0
68	5536.0
69	4624.5
70	3713.0
71	3269.5
72	2826.0
73	2285.0
74	1744.0
75	1321.5
76	899.0
77	605.0
78	311.0
79	268.5
80	226.0
81	192.5
82	159.0
83	104.5
84	50.0
85	44.5
86	39.0
87	21.5
88	4.0
89	3.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1888999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.088368918742862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.58456799595685	16.572203612187714
2	8.211366895030164	3.4632866881909634
3	3.013331253082736	1.9063872341815935
4	1.613187754463428	1.360779940052926
5	0.9900482935924931	1.043925183132517
6	0.7629819147652867	0.9654026458139512
7	0.5610828587643396	0.8282625623723703
8	0.4707083278545714	0.7941176696737414
9	0.4040064573277926	0.7667853495914553
>10	4.087888197664648	19.303964415854075
>50	0.664458500736759	9.636053963927308
>100	0.5473402368588728	24.724233179269767
>500	0.07410845162868793	10.17869346828842
>1k	0.013658212928154088	4.720055663889928
>5k	5.058597380797811E-4	0.7168199166819231
>10k+	7.587896071196715E-4	3.0190285068914005
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29980	1.5870839529295675	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	14631	0.7745372019784023	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	11990	0.6347277049908444	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	6962	0.36855498600052194	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	6477	0.34288001211223507	No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4829	0.2556380389825511	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4123	0.21826374709568402	No Hit
AGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3302	0.17480157480231595	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2751	0.1456326869416024	No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2716	0.14377985377440644	No Hit
AGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2640	0.13975655889706665	No Hit
GGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	2600	0.1376390352774141	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2520	0.13340398803810907	No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2276	0.12048709395822868	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	2227	0.11789312752415433	No Hit
TAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2140	0.11328751365141009	No Hit
AAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2027	0.10730550942589169	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	1928	0.1020646384672517	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04875598134249939	0.0
2	0.0	0.0	0.0	0.20979365261707392	0.0
3	0.0	0.0	0.0	0.30598216303978987	0.0
4	0.0	0.0	0.0	0.46299653943702457	0.0
5	0.0	0.0	0.0	0.8055059849158205	0.0
6	0.0	0.0	0.0	1.1191641710768507	0.0
7	0.0	0.0	0.0	1.3076237732259255	0.0
8	0.0	0.0	0.0	1.7830607639284086	0.0
9	0.0	0.0	0.0	1.9877194217678251	0.0
10	0.0	0.0	0.0	2.393754575836197	0.0
11	0.0	0.0	0.0	2.8967193735941628	0.0
12	0.0	0.0	0.0	3.2750149682450864	0.0
13	0.0	0.0	0.0	3.4232945597112545	0.0
14	0.0	0.0	0.0	3.485073311314617	0.0
15	0.0	0.0	0.0	3.5752798175118143	0.0
16	0.0	0.0	0.0	3.7803090419846703	0.0
17	0.0	0.0	0.0	4.000584436519024	0.0
18	0.0	0.0	0.0	4.2823738922042835	0.0
19	0.0	0.0	0.0	4.435841416538601	0.0
20	0.0	0.0	0.0	4.604555110934416	0.0
21	0.0	0.0	0.0	4.8199602011435685	0.0
22	0.0	0.0	0.0	5.060140317702657	0.0
23	0.0	0.0	0.0	5.298467601094548	0.0
24	0.0	0.0	0.0	5.501643992400208	0.0
25	0.0	0.0	0.0	5.6874566900247165	0.0
26	0.0	0.0	0.0	5.8576526509542886	0.0
27	0.0	0.0	0.0	6.032454225756604	0.0
28	0.0	0.0	0.0	6.21127909543626	0.0
29	0.0	0.0	0.0	6.398679935775508	0.0
30	0.0	0.0	0.0	6.642671594849971	0.0
31	0.0	0.0	0.0	6.860564775312215	0.0
32	0.0	0.0	0.0	7.081369550751482	0.0
33	0.0	0.0	0.0	7.274328890592319	0.0
34	0.0	0.0	0.0	7.485710685924133	0.0
35	0.0	0.0	0.0	7.751618714461998	0.0
36	0.0	0.0	0.0	7.989098988406028	0.0
37	0.0	0.0	0.0	8.219591434405206	0.0
38	0.0	0.0	0.0	8.439125695672681	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGCGT	65	0.0	44.000004	5
TAATACG	40	8.3182385E-9	44.0	1
TACCCGC	50	2.7284841E-11	44.0	30
CTCCGTA	20	7.85777E-4	44.0	17
ACGTTAG	35	1.4467514E-7	44.0	1
CGCATTA	20	7.85777E-4	44.0	21
GCACGAG	55	1.8189894E-12	44.0	1
CTAAGCG	50	2.7284841E-11	44.0	1
ACTTACC	30	2.5285863E-6	44.0	42
TAGCACG	20	7.85777E-4	44.0	1
AACGGAT	20	7.85777E-4	44.0	17
CTAACGG	30	2.5285863E-6	44.0	2
TATCTCG	20	7.85777E-4	44.0	1
TCGCTAA	35	1.4467514E-7	44.0	27
GTCGTAG	60	0.0	44.0	1
TTAACGA	30	2.5285863E-6	44.0	40
CGTAATC	50	2.7284841E-11	44.0	38
TATAGCG	110	0.0	44.0	1
GGACCGT	60	0.0	44.0	8
ATACGAC	25	4.443932E-5	44.0	24
>>END_MODULE