FastQCFastQC Report
Thu 26 May 2016
SRR1546167_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546167_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences7079983
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2506953.5408983326654884No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1362800.043.2477261
CGGTCTA9200.041.84782831
CGTAACG452.3539542E-839.111111
ACGGTCT10150.038.36453230
GTTTTTT1574600.038.2492072
TCACGAC10300.037.8058325
CGACGGT10450.037.6842128
CACGACG10350.037.62318826
TAGGGAC56250.036.7644425
AGGGCGA47950.036.7507826
CGGGTAT11450.036.506556
CATACGA23950.036.1920718
CTATACG1400.036.1428571
TATACGG7550.036.1324542
ATAGGGA73900.035.6644134
GGCGATT28350.035.619058
GTAGGGC30350.035.591434
GGGCGAT89650.035.460127
GTACGGG20350.035.459463
AACGGGA20300.035.4384234
Produced by FastQC (version 0.11.5)