##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546167_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	7079983
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96150583977391	33.0	31.0	34.0	30.0	34.0
2	32.31151049373989	33.0	31.0	34.0	31.0	34.0
3	32.09154287517357	33.0	31.0	34.0	30.0	34.0
4	35.94669634093754	37.0	35.0	37.0	35.0	37.0
5	35.9812321583258	37.0	35.0	37.0	35.0	37.0
6	36.02927323413065	37.0	35.0	37.0	35.0	37.0
7	36.22024389041612	37.0	35.0	37.0	35.0	37.0
8	36.11898051167637	37.0	36.0	37.0	35.0	37.0
9	37.86692312679282	39.0	38.0	39.0	35.0	39.0
10	37.578799129884914	39.0	37.0	39.0	35.0	39.0
11	37.49449299525154	39.0	37.0	39.0	35.0	39.0
12	37.36372389594721	39.0	37.0	39.0	35.0	39.0
13	37.321308116135306	39.0	37.0	39.0	34.0	39.0
14	38.650921336958014	40.0	38.0	41.0	34.0	41.0
15	38.665350043919595	40.0	38.0	41.0	34.0	41.0
16	38.66725711064561	40.0	38.0	41.0	34.0	41.0
17	38.55498000489549	40.0	38.0	41.0	34.0	41.0
18	38.193424475736734	39.0	37.0	41.0	34.0	41.0
19	37.83049493197936	39.0	37.0	41.0	34.0	41.0
20	37.43807026090317	39.0	35.0	41.0	34.0	41.0
21	37.349382477330806	39.0	35.0	41.0	33.0	41.0
22	37.25851234388557	39.0	35.0	41.0	33.0	41.0
23	37.17286171449847	39.0	35.0	41.0	33.0	41.0
24	37.09712226710149	38.0	35.0	41.0	33.0	41.0
25	37.072735767868366	38.0	35.0	41.0	33.0	41.0
26	37.01259861782154	38.0	35.0	41.0	33.0	41.0
27	36.93800663080688	38.0	35.0	41.0	33.0	41.0
28	36.83069648613563	38.0	35.0	41.0	33.0	41.0
29	36.71481880676832	38.0	35.0	41.0	33.0	41.0
30	36.47769309615574	38.0	35.0	41.0	31.0	41.0
31	36.12780736902899	38.0	35.0	41.0	30.0	41.0
32	35.595160327362365	38.0	35.0	41.0	27.0	41.0
33	34.92496733396111	38.0	35.0	41.0	21.0	41.0
34	34.168420037166754	38.0	34.0	41.0	17.0	41.0
35	33.718103419174874	38.0	33.0	41.0	15.0	41.0
36	33.52349306488448	38.0	33.0	41.0	10.0	41.0
37	33.426723199759095	38.0	33.0	41.0	10.0	41.0
38	33.295137431827165	38.0	33.0	41.0	10.0	41.0
39	33.15919176642091	38.0	33.0	40.0	10.0	41.0
40	33.06153687092186	38.0	33.0	40.0	10.0	41.0
41	32.89040086678174	38.0	32.0	40.0	10.0	41.0
42	32.80798061238283	38.0	32.0	40.0	10.0	41.0
43	32.661577294747744	37.0	32.0	40.0	10.0	41.0
44	32.499336792192864	37.0	31.0	40.0	10.0	41.0
45	32.43166530202121	37.0	31.0	40.0	10.0	41.0
46	32.35239095913084	37.0	31.0	40.0	10.0	41.0
47	32.30371683095849	37.0	31.0	40.0	10.0	41.0
48	32.193817697019895	36.0	31.0	40.0	9.0	41.0
49	32.104171012840006	36.0	31.0	40.0	8.0	41.0
50	31.96894653560609	36.0	30.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	8.0
10	11.0
11	18.0
12	35.0
13	38.0
14	90.0
15	187.0
16	461.0
17	1231.0
18	2949.0
19	6196.0
20	11237.0
21	18156.0
22	28424.0
23	43757.0
24	71684.0
25	129000.0
26	221397.0
27	280161.0
28	260005.0
29	213249.0
30	182465.0
31	175683.0
32	187892.0
33	224371.0
34	356800.0
35	564112.0
36	467901.0
37	569282.0
38	998151.0
39	2065028.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.952906101610697	18.246103133298483	24.058122738430303	29.742868026660517
2	43.67242124733915	20.973087082271245	25.46155831165131	9.892933358738292
3	23.86110249134779	21.47010804969447	44.105473134610634	10.563316324347106
4	21.6830040411114	23.101114790812353	43.547957671649776	11.667923496426475
5	19.163746579617495	25.848353025706416	43.06797064343233	11.919929751243753
6	17.396171713971633	32.965065029110946	40.967160514368466	8.671602742548957
7	70.25279580473568	4.096690627646987	23.71872926813525	1.931784299482075
8	70.94084830429678	3.7502067448467034	23.461694752656893	1.8472501981996285
9	66.09538469230787	5.656087027327608	25.14756038256024	3.1009678978042743
10	29.784506544719104	27.096082010366406	31.583988266638492	11.535423178275993
11	24.008701715809206	22.68690475669221	39.58279278354199	13.72160074395659
12	22.79895304833359	20.30473801985118	42.43255951320787	14.46374941860736
13	18.440764617655155	22.959348913690896	42.95934043909428	15.640546029559676
14	15.851860096274242	24.8939863273683	43.08838029695834	16.16577327939912
15	15.764656497056562	22.39019500470552	46.86461817775551	14.980530320482407
16	17.861130457516637	21.10914673100204	45.01416175716806	16.015561054313267
17	17.700183743379043	21.56147832558355	42.11994576823137	18.618392162806042
18	18.63897130826444	22.196974201774214	42.88333743174242	16.280717058218926
19	20.14869244742537	23.424138165303503	39.741592599869236	16.68557678740189
20	21.770744364781667	21.726916575929632	41.031313210780304	15.471025848508393
21	19.93870606751457	22.998699290662138	41.42836218674537	15.634232455077928
22	19.997632762677537	21.437014749894175	40.52343063535604	18.041921852072242
23	18.2907501331571	23.22303598751579	41.25474595066118	17.231467928665932
24	17.82307951869376	21.72989680907426	44.43168860716191	16.015335065070072
25	18.20505783700328	23.425649468367368	41.71481767682211	16.654475017807247
26	17.318219549397224	24.738661095655175	40.66422193386622	17.27889742108138
27	16.918486951169236	24.36329296270909	40.9329513926799	17.78526869344178
28	16.080165729211497	23.88987374687199	42.91610587200562	17.113854651910888
29	17.83579141362345	22.22251098625519	42.16292609742142	17.778771502699936
30	18.089817447301783	24.852658544519105	40.7887278825387	16.268796125640414
31	19.81109276674817	24.576881046183303	39.16866749538806	16.443358691680473
32	20.894004971480864	23.694901527305927	38.10520448989779	17.305889011315422
33	20.41569873825968	25.099198684516615	37.07466529227542	17.410437284948284
34	18.84980514783722	24.937390386389346	37.94979451221846	18.263009953554974
35	19.66487772640132	25.952322201903595	36.529155507859265	17.853644563835815
36	20.352082766300427	28.196112900271086	34.07721459218193	17.374589741246556
37	19.840993968488345	28.69176380790745	34.53424958788743	16.932992635716783
38	19.364114292364825	28.66125526007619	34.16648034324376	17.808150104315224
39	19.815358313713467	28.563952766553253	33.28143584525556	18.33925307447772
40	20.65941401271726	27.10380801761812	33.95640921736677	18.28036875229785
41	18.36284070173615	27.86087198231973	33.374557537779395	20.40172977816472
42	19.122941396893182	27.904600900877867	32.18777502714343	20.78468267508552
43	19.335978631587107	27.728696523706343	33.18765030932984	19.747674535376706
44	19.58299899872641	28.106437543705965	32.34774716266974	19.962816294897884
45	19.078506261950064	29.011510338372283	31.96324058970198	19.946742809975675
46	19.626516052369052	29.053191229413972	32.75530463844334	18.564988079773638
47	19.322037920147547	28.105519462405486	33.603894246638724	18.968548370808236
48	19.176868080050475	26.46928672003874	34.590450287804366	19.763394912106428
49	19.087065604536054	26.748496430005552	34.36742715342678	19.79701081203161
50	18.795497107832038	27.541139576182598	34.30826034469292	19.355102971292446
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8666.0
1	15498.5
2	22331.0
3	215062.0
4	407793.0
5	277163.5
6	146534.0
7	141840.5
8	137147.0
9	136883.0
10	136619.0
11	134747.0
12	132875.0
13	126402.0
14	119929.0
15	114739.0
16	109549.0
17	101263.5
18	92978.0
19	88501.5
20	84025.0
21	82037.5
22	80050.0
23	81763.0
24	83476.0
25	86914.5
26	90353.0
27	97077.5
28	103802.0
29	121407.0
30	139012.0
31	165379.0
32	191746.0
33	212932.5
34	234119.0
35	254129.0
36	274139.0
37	282720.0
38	291301.0
39	305540.0
40	319779.0
41	333793.0
42	347807.0
43	362921.0
44	378035.0
45	385775.0
46	393515.0
47	402651.0
48	411787.0
49	406328.5
50	400870.0
51	372109.5
52	343349.0
53	316070.0
54	288791.0
55	275491.0
56	262191.0
57	256702.5
58	251214.0
59	241506.5
60	231799.0
61	207882.0
62	183965.0
63	160664.0
64	137363.0
65	112094.0
66	86825.0
67	71087.0
68	55349.0
69	49460.5
70	43572.0
71	34731.5
72	25891.0
73	20253.5
74	14616.0
75	11422.0
76	8228.0
77	6227.0
78	4226.0
79	3253.5
80	2281.0
81	1664.0
82	1047.0
83	759.5
84	472.0
85	377.0
86	282.0
87	196.5
88	111.0
89	123.5
90	136.0
91	80.5
92	25.0
93	15.0
94	5.0
95	4.0
96	3.0
97	2.0
98	1.0
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	7079983.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.09585720737133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.65173554187321	16.006699037687884
2	7.404757369327253	2.976098934984621
3	2.635847126714934	1.5890882243676998
4	1.3902960751799052	1.1175676561113668
5	0.9014925779176952	0.905813305966954
6	0.6635452309450689	0.8000706127022604
7	0.4969512726039761	0.699066326928968
8	0.4109723373890226	0.6607073126679547
9	0.36420871505492386	0.6587177698281549
>10	3.9709760386032813	18.28673238699976
>50	1.200144660577343	17.165263564168818
>100	0.8855782005810133	31.14905273484194
>500	0.019197014785659355	2.425565841565135
>1k	0.004011315883662574	1.722005697387002
>5k	2.1489192233906443E-4	0.2288483918936229
>10k+	7.163064077968814E-5	3.608702201897836
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	250695	3.5408983326654884	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03025430993266509	0.0
2	0.0	0.0	0.0	0.10146917019433521	0.0
3	0.0	0.0	0.0	0.18779706109463823	0.0
4	0.0	0.0	0.0	0.26837635062118087	0.0
5	0.0	0.0	0.0	0.49793904872370454	0.0
6	0.0	0.0	0.0	0.8410754658591695	0.0
7	0.0	0.0	0.0	1.047050536703266	0.0
8	0.0	0.0	0.0	1.413195483661472	0.0
9	0.0	0.0	0.0	1.671359945355801	0.0
10	0.0	0.0	0.0	2.0046093331015062	0.0
11	0.0	0.0	0.0	2.277067614427888	0.0
12	0.0	0.0	0.0	2.4994551540589858	0.0
13	1.4124327699656906E-5	0.0	0.0	2.6073650176843644	0.0
14	1.4124327699656906E-5	0.0	0.0	2.665528999151552	0.0
15	1.4124327699656906E-5	0.0	0.0	2.718664719957661	0.0
16	1.4124327699656906E-5	0.0	0.0	2.805684702915247	0.0
17	1.4124327699656906E-5	0.0	0.0	2.9020973637931053	0.0
18	1.4124327699656906E-5	0.0	0.0	3.034710676565184	0.0
19	1.4124327699656906E-5	0.0	0.0	3.113580922440068	0.0
20	1.4124327699656906E-5	0.0	0.0	3.202747803208002	0.0
21	1.4124327699656906E-5	0.0	0.0	3.3076774336887533	0.0
22	1.4124327699656906E-5	0.0	0.0	3.424768110318909	0.0
23	1.4124327699656906E-5	0.0	0.0	3.5450932579922863	0.0
24	1.4124327699656906E-5	0.0	0.0	3.6448251358795636	0.0
25	1.4124327699656906E-5	0.0	0.0	3.730686923965778	0.0
26	1.4124327699656906E-5	0.0	0.0	3.8148114197449345	0.0
27	1.4124327699656906E-5	0.0	0.0	3.90143592152693	0.0
28	1.4124327699656906E-5	0.0	0.0	3.9970999930367066	0.0
29	1.4124327699656906E-5	1.4124327699656906E-5	0.0	4.099190633649827	0.0
30	1.4124327699656906E-5	1.4124327699656906E-5	0.0	4.231154227347721	0.0
31	1.4124327699656906E-5	1.4124327699656906E-5	0.0	4.338569739503612	0.0
32	1.4124327699656906E-5	1.4124327699656906E-5	0.0	4.452468318073645	0.0
33	1.4124327699656906E-5	1.4124327699656906E-5	0.0	4.56506745849531	0.0
34	1.4124327699656906E-5	1.4124327699656906E-5	0.0	4.67951688584563	0.0
35	2.824865539931381E-5	1.4124327699656906E-5	0.0	4.810181041395156	0.0
36	2.824865539931381E-5	1.4124327699656906E-5	0.0	4.935195465864819	0.0
37	2.824865539931381E-5	1.4124327699656906E-5	0.0	5.0540234348020325	0.0
38	2.824865539931381E-5	1.4124327699656906E-5	0.0	5.183656514429484	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	136280	0.0	43.247726	1
CGGTCTA	920	0.0	41.847828	31
CGTAACG	45	2.3539542E-8	39.11111	1
ACGGTCT	1015	0.0	38.364532	30
GTTTTTT	157460	0.0	38.249207	2
TCACGAC	1030	0.0	37.80583	25
CGACGGT	1045	0.0	37.68421	28
CACGACG	1035	0.0	37.623188	26
TAGGGAC	5625	0.0	36.764442	5
AGGGCGA	4795	0.0	36.750782	6
CGGGTAT	1145	0.0	36.50655	6
CATACGA	2395	0.0	36.19207	18
CTATACG	140	0.0	36.142857	1
TATACGG	755	0.0	36.132454	2
ATAGGGA	7390	0.0	35.664413	4
GGCGATT	2835	0.0	35.61905	8
GTAGGGC	3035	0.0	35.59143	4
GGGCGAT	8965	0.0	35.46012	7
GTACGGG	2035	0.0	35.45946	3
AACGGGA	2030	0.0	35.438423	4
>>END_MODULE